Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS18960

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS18950 and BPHYT_RS18955 are separated by 15 nucleotidesBPHYT_RS18955 and BPHYT_RS18960 are separated by 288 nucleotidesBPHYT_RS18960 and BPHYT_RS18965 are separated by 1 nucleotidesBPHYT_RS18965 and BPHYT_RS18970 are separated by 86 nucleotides BPHYT_RS18950: BPHYT_RS18950 - hypothetical protein, at 4,257,029 to 4,258,318 _RS18950 BPHYT_RS18955: BPHYT_RS18955 - hypothetical protein, at 4,258,334 to 4,258,879 _RS18955 BPHYT_RS18960: BPHYT_RS18960 - chemotaxis protein CheZ, at 4,259,168 to 4,259,878 _RS18960 BPHYT_RS18965: BPHYT_RS18965 - chemotaxis protein CheY, at 4,259,880 to 4,260,275 _RS18965 BPHYT_RS18970: BPHYT_RS18970 - chemotaxis protein, at 4,260,362 to 4,261,450 _RS18970 Position (kb) 4259 4260Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2at 4258.303 kb on + strandat 4258.303 kb on + strandat 4258.303 kb on + strandat 4258.303 kb on + strandat 4258.303 kb on + strandat 4258.303 kb on + strandat 4258.304 kb on - strandat 4258.332 kb on + strandat 4258.336 kb on - strandat 4258.506 kb on + strand, within BPHYT_RS18955at 4258.519 kb on - strand, within BPHYT_RS18955at 4258.647 kb on + strand, within BPHYT_RS18955at 4258.647 kb on + strand, within BPHYT_RS18955at 4258.648 kb on - strand, within BPHYT_RS18955at 4258.648 kb on - strand, within BPHYT_RS18955at 4258.742 kb on - strand, within BPHYT_RS18955at 4258.742 kb on - strand, within BPHYT_RS18955at 4258.937 kb on + strandat 4258.937 kb on + strandat 4258.937 kb on + strandat 4258.937 kb on + strandat 4258.938 kb on - strandat 4258.938 kb on - strandat 4258.938 kb on - strandat 4258.938 kb on - strandat 4258.938 kb on - strandat 4258.949 kb on - strandat 4259.046 kb on + strandat 4259.047 kb on - strandat 4259.047 kb on - strandat 4259.047 kb on - strandat 4259.049 kb on + strandat 4259.049 kb on + strandat 4259.049 kb on + strandat 4259.049 kb on + strandat 4259.049 kb on + strandat 4259.050 kb on - strandat 4259.050 kb on - strandat 4259.103 kb on + strandat 4259.103 kb on + strandat 4259.103 kb on + strandat 4259.103 kb on + strandat 4259.103 kb on + strandat 4259.103 kb on + strandat 4259.104 kb on - strandat 4259.104 kb on - strandat 4259.104 kb on - strandat 4259.104 kb on - strandat 4259.388 kb on + strand, within BPHYT_RS18960at 4259.389 kb on - strand, within BPHYT_RS18960at 4259.389 kb on - strand, within BPHYT_RS18960at 4259.688 kb on + strand, within BPHYT_RS18960at 4259.689 kb on - strand, within BPHYT_RS18960at 4259.963 kb on - strand, within BPHYT_RS18965at 4260.128 kb on - strand, within BPHYT_RS18965at 4260.131 kb on + strandat 4260.365 kb on + strandat 4260.366 kb on - strandat 4260.491 kb on - strand, within BPHYT_RS18970at 4260.491 kb on - strand, within BPHYT_RS18970at 4260.520 kb on - strand, within BPHYT_RS18970at 4260.520 kb on - strand, within BPHYT_RS18970at 4260.689 kb on - strand, within BPHYT_RS18970at 4260.689 kb on - strand, within BPHYT_RS18970at 4260.689 kb on - strand, within BPHYT_RS18970at 4260.690 kb on + strand, within BPHYT_RS18970at 4260.690 kb on + strand, within BPHYT_RS18970at 4260.691 kb on - strand, within BPHYT_RS18970at 4260.691 kb on - strand, within BPHYT_RS18970at 4260.691 kb on - strand, within BPHYT_RS18970at 4260.741 kb on + strand, within BPHYT_RS18970at 4260.741 kb on + strand, within BPHYT_RS18970at 4260.741 kb on + strand, within BPHYT_RS18970at 4260.741 kb on + strand, within BPHYT_RS18970at 4260.741 kb on + strand, within BPHYT_RS18970at 4260.741 kb on + strand, within BPHYT_RS18970at 4260.742 kb on - strand, within BPHYT_RS18970at 4260.742 kb on - strand, within BPHYT_RS18970at 4260.742 kb on - strand, within BPHYT_RS18970at 4260.742 kb on - strand, within BPHYT_RS18970at 4260.742 kb on - strand, within BPHYT_RS18970at 4260.784 kb on + strand, within BPHYT_RS18970at 4260.791 kb on - strand, within BPHYT_RS18970at 4260.847 kb on + strand, within BPHYT_RS18970at 4260.847 kb on + strand, within BPHYT_RS18970at 4260.847 kb on + strand, within BPHYT_RS18970at 4260.848 kb on - strand, within BPHYT_RS18970at 4260.848 kb on - strand, within BPHYT_RS18970at 4260.848 kb on - strand, within BPHYT_RS18970

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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4,258,303 + -0.7
4,258,303 + -0.1
4,258,303 + +0.3
4,258,303 + +0.1
4,258,303 + -0.0
4,258,303 + -0.1
4,258,304 - +0.1
4,258,332 + -0.3
4,258,336 - +0.5
4,258,506 + BPHYT_RS18955 0.32 +0.1
4,258,519 - BPHYT_RS18955 0.34 +0.3
4,258,647 + BPHYT_RS18955 0.57 -1.0
4,258,647 + BPHYT_RS18955 0.57 -0.1
4,258,648 - BPHYT_RS18955 0.58 -0.4
4,258,648 - BPHYT_RS18955 0.58 -2.0
4,258,742 - BPHYT_RS18955 0.75 +0.1
4,258,742 - BPHYT_RS18955 0.75 +1.5
4,258,937 + +0.0
4,258,937 + +0.5
4,258,937 + +0.5
4,258,937 + +0.1
4,258,938 - -0.8
4,258,938 - +2.3
4,258,938 - +0.6
4,258,938 - +0.1
4,258,938 - -1.3
4,258,949 - -1.4
4,259,046 + +0.2
4,259,047 - +0.3
4,259,047 - -0.3
4,259,047 - +0.2
4,259,049 + -0.3
4,259,049 + +1.0
4,259,049 + +0.4
4,259,049 + -0.3
4,259,049 + -0.1
4,259,050 - +0.5
4,259,050 - +0.2
4,259,103 + -0.0
4,259,103 + -0.4
4,259,103 + +0.6
4,259,103 + -0.1
4,259,103 + -0.0
4,259,103 + -2.1
4,259,104 - +0.6
4,259,104 - +0.2
4,259,104 - +0.7
4,259,104 - +0.8
4,259,388 + BPHYT_RS18960 0.31 -1.2
4,259,389 - BPHYT_RS18960 0.31 +0.7
4,259,389 - BPHYT_RS18960 0.31 -0.4
4,259,688 + BPHYT_RS18960 0.73 -0.9
4,259,689 - BPHYT_RS18960 0.73 -1.0
4,259,963 - BPHYT_RS18965 0.21 -0.6
4,260,128 - BPHYT_RS18965 0.63 -1.3
4,260,131 + -0.9
4,260,365 + -0.9
4,260,366 - -0.9
4,260,491 - BPHYT_RS18970 0.12 -4.3
4,260,491 - BPHYT_RS18970 0.12 -1.9
4,260,520 - BPHYT_RS18970 0.15 -3.8
4,260,520 - BPHYT_RS18970 0.15 -3.2
4,260,689 - BPHYT_RS18970 0.30 -3.5
4,260,689 - BPHYT_RS18970 0.30 -2.9
4,260,689 - BPHYT_RS18970 0.30 -3.5
4,260,690 + BPHYT_RS18970 0.30 -3.0
4,260,690 + BPHYT_RS18970 0.30 -2.5
4,260,691 - BPHYT_RS18970 0.30 -3.7
4,260,691 - BPHYT_RS18970 0.30 -3.2
4,260,691 - BPHYT_RS18970 0.30 -1.7
4,260,741 + BPHYT_RS18970 0.35 -2.3
4,260,741 + BPHYT_RS18970 0.35 -2.3
4,260,741 + BPHYT_RS18970 0.35 -3.4
4,260,741 + BPHYT_RS18970 0.35 -5.3
4,260,741 + BPHYT_RS18970 0.35 -1.9
4,260,741 + BPHYT_RS18970 0.35 -2.3
4,260,742 - BPHYT_RS18970 0.35 -4.4
4,260,742 - BPHYT_RS18970 0.35 -0.9
4,260,742 - BPHYT_RS18970 0.35 -3.8
4,260,742 - BPHYT_RS18970 0.35 -1.6
4,260,742 - BPHYT_RS18970 0.35 -3.9
4,260,784 + BPHYT_RS18970 0.39 -3.9
4,260,791 - BPHYT_RS18970 0.39 -3.9
4,260,847 + BPHYT_RS18970 0.45 -4.9
4,260,847 + BPHYT_RS18970 0.45 -5.9
4,260,847 + BPHYT_RS18970 0.45 -2.1
4,260,848 - BPHYT_RS18970 0.45 -3.7
4,260,848 - BPHYT_RS18970 0.45 -4.7
4,260,848 - BPHYT_RS18970 0.45 -3.6

Or see this region's nucleotide sequence