Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18900 and BPHYT_RS18905 are separated by 201 nucleotides BPHYT_RS18905 and BPHYT_RS18910 are separated by 94 nucleotides BPHYT_RS18910 and BPHYT_RS18915 are separated by 308 nucleotides
BPHYT_RS18900: BPHYT_RS18900 - flagella basal body P-ring formation protein FlgA, at 4,246,027 to 4,247,541
_RS18900
BPHYT_RS18905: BPHYT_RS18905 - anti-sigma-28 factor FlgM, at 4,247,743 to 4,248,096
_RS18905
BPHYT_RS18910: BPHYT_RS18910 - FlgN, at 4,248,191 to 4,248,637
_RS18910
BPHYT_RS18915: BPHYT_RS18915 - hypothetical protein, at 4,248,946 to 4,249,128
_RS18915
Position (kb) 4247
4248
4249 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 4247.051 kb on + strand, within BPHYT_RS18900 at 4247.051 kb on + strand, within BPHYT_RS18900 at 4247.137 kb on + strand, within BPHYT_RS18900 at 4247.137 kb on + strand, within BPHYT_RS18900 at 4247.138 kb on - strand, within BPHYT_RS18900 at 4247.170 kb on + strand, within BPHYT_RS18900 at 4247.170 kb on + strand, within BPHYT_RS18900 at 4247.170 kb on + strand, within BPHYT_RS18900 at 4247.170 kb on + strand, within BPHYT_RS18900 at 4247.170 kb on + strand, within BPHYT_RS18900 at 4247.170 kb on + strand, within BPHYT_RS18900 at 4247.171 kb on - strand, within BPHYT_RS18900 at 4247.171 kb on - strand, within BPHYT_RS18900 at 4247.171 kb on - strand, within BPHYT_RS18900 at 4247.171 kb on - strand, within BPHYT_RS18900 at 4247.171 kb on - strand at 4247.171 kb on - strand, within BPHYT_RS18900 at 4247.173 kb on + strand, within BPHYT_RS18900 at 4247.173 kb on + strand, within BPHYT_RS18900 at 4247.173 kb on + strand, within BPHYT_RS18900 at 4247.173 kb on + strand, within BPHYT_RS18900 at 4247.174 kb on - strand, within BPHYT_RS18900 at 4247.174 kb on - strand, within BPHYT_RS18900 at 4247.174 kb on - strand, within BPHYT_RS18900 at 4247.174 kb on - strand, within BPHYT_RS18900 at 4247.174 kb on - strand, within BPHYT_RS18900 at 4247.174 kb on - strand, within BPHYT_RS18900 at 4247.174 kb on - strand, within BPHYT_RS18900 at 4247.174 kb on - strand, within BPHYT_RS18900 at 4247.656 kb on + strand at 4247.656 kb on + strand at 4247.657 kb on - strand at 4247.657 kb on - strand at 4247.657 kb on - strand at 4247.666 kb on + strand at 4247.712 kb on + strand at 4247.714 kb on + strand at 4247.714 kb on + strand at 4247.714 kb on + strand at 4248.111 kb on + strand at 4248.503 kb on - strand, within BPHYT_RS18910 at 4248.539 kb on + strand, within BPHYT_RS18910 at 4248.540 kb on - strand, within BPHYT_RS18910 at 4248.596 kb on + strand at 4248.597 kb on - strand at 4248.775 kb on - strand at 4248.837 kb on + strand at 4248.837 kb on + strand at 4248.838 kb on - strand at 4248.896 kb on + strand at 4248.945 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 4,247,051 + BPHYT_RS18900 0.68 -2.6 4,247,051 + BPHYT_RS18900 0.68 -3.8 4,247,137 + BPHYT_RS18900 0.73 -3.0 4,247,137 + BPHYT_RS18900 0.73 -3.0 4,247,138 - BPHYT_RS18900 0.73 -3.7 4,247,170 + BPHYT_RS18900 0.75 -2.0 4,247,170 + BPHYT_RS18900 0.75 -3.5 4,247,170 + BPHYT_RS18900 0.75 -1.9 4,247,170 + BPHYT_RS18900 0.75 -2.3 4,247,170 + BPHYT_RS18900 0.75 -2.2 4,247,170 + BPHYT_RS18900 0.75 -5.1 4,247,171 - BPHYT_RS18900 0.76 -3.3 4,247,171 - BPHYT_RS18900 0.76 -4.4 4,247,171 - BPHYT_RS18900 0.76 -3.0 4,247,171 - BPHYT_RS18900 0.76 -2.3 4,247,171 - +0.7 4,247,171 - BPHYT_RS18900 0.76 -3.6 4,247,173 + BPHYT_RS18900 0.76 -0.3 4,247,173 + BPHYT_RS18900 0.76 -3.9 4,247,173 + BPHYT_RS18900 0.76 -2.0 4,247,173 + BPHYT_RS18900 0.76 -2.4 4,247,174 - BPHYT_RS18900 0.76 -4.4 4,247,174 - BPHYT_RS18900 0.76 -1.3 4,247,174 - BPHYT_RS18900 0.76 -3.2 4,247,174 - BPHYT_RS18900 0.76 -1.6 4,247,174 - BPHYT_RS18900 0.76 -2.1 4,247,174 - BPHYT_RS18900 0.76 -4.3 4,247,174 - BPHYT_RS18900 0.76 -1.6 4,247,174 - BPHYT_RS18900 0.76 -2.6 4,247,656 + -0.5 4,247,656 + +0.0 4,247,657 - -0.9 4,247,657 - -0.9 4,247,657 - -1.1 4,247,666 + +0.1 4,247,712 + -0.1 4,247,714 + -0.1 4,247,714 + -0.3 4,247,714 + -1.3 4,248,111 + +0.8 4,248,503 - BPHYT_RS18910 0.70 -1.9 4,248,539 + BPHYT_RS18910 0.78 -3.0 4,248,540 - BPHYT_RS18910 0.78 -2.9 4,248,596 + -3.9 4,248,597 - -2.3 4,248,775 - +0.3 4,248,837 + +1.6 4,248,837 + -0.5 4,248,838 - +0.2 4,248,896 + +0.0 4,248,945 + -0.5
Or see this region's nucleotide sequence