Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS18870

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS18860 and BPHYT_RS18865 are separated by 2 nucleotidesBPHYT_RS18865 and BPHYT_RS18870 are separated by 36 nucleotidesBPHYT_RS18870 and BPHYT_RS18875 are separated by 12 nucleotidesBPHYT_RS18875 and BPHYT_RS18880 are separated by 20 nucleotides BPHYT_RS18860: BPHYT_RS18860 - flagellar P-ring protein FlgI, at 4,239,004 to 4,240,191 _RS18860 BPHYT_RS18865: BPHYT_RS18865 - flagellar L-ring protein FlgH, at 4,240,194 to 4,240,892 _RS18865 BPHYT_RS18870: BPHYT_RS18870 - flagellar basal body rod protein FlgG, at 4,240,929 to 4,241,717 _RS18870 BPHYT_RS18875: BPHYT_RS18875 - flagellar basal body rod protein FlgF, at 4,241,730 to 4,242,488 _RS18875 BPHYT_RS18880: BPHYT_RS18880 - flagellar hook protein FlgE, at 4,242,509 to 4,244,020 _RS18880 Position (kb) 4240 4241 4242Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 4240.080 kb on - strandat 4240.185 kb on + strandat 4240.186 kb on - strandat 4240.238 kb on + strandat 4240.256 kb on + strandat 4240.258 kb on + strandat 4240.259 kb on - strandat 4240.259 kb on - strandat 4240.259 kb on - strandat 4240.307 kb on - strand, within BPHYT_RS18865at 4240.307 kb on - strand, within BPHYT_RS18865at 4240.717 kb on + strand, within BPHYT_RS18865at 4240.875 kb on - strandat 4240.909 kb on + strandat 4240.930 kb on + strandat 4240.930 kb on + strandat 4240.930 kb on + strandat 4240.931 kb on - strandat 4241.000 kb on - strandat 4241.065 kb on + strand, within BPHYT_RS18870at 4241.065 kb on + strand, within BPHYT_RS18870at 4241.066 kb on - strand, within BPHYT_RS18870at 4241.378 kb on - strand, within BPHYT_RS18870at 4241.476 kb on + strand, within BPHYT_RS18870at 4241.477 kb on - strand, within BPHYT_RS18870at 4241.582 kb on - strand, within BPHYT_RS18870at 4241.701 kb on + strandat 4241.701 kb on + strandat 4241.701 kb on + strandat 4241.702 kb on - strandat 4241.735 kb on + strandat 4241.736 kb on - strandat 4241.736 kb on - strandat 4242.175 kb on + strand, within BPHYT_RS18875at 4242.176 kb on - strand, within BPHYT_RS18875at 4242.274 kb on + strand, within BPHYT_RS18875at 4242.274 kb on + strand, within BPHYT_RS18875at 4242.275 kb on - strand, within BPHYT_RS18875at 4242.511 kb on - strandat 4242.511 kb on - strandat 4242.574 kb on - strandat 4242.574 kb on - strandat 4242.574 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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4,240,080 - -4.8
4,240,185 + -5.0
4,240,186 - -3.7
4,240,238 + -5.0
4,240,256 + -4.8
4,240,258 + -2.1
4,240,259 - -2.4
4,240,259 - -1.0
4,240,259 - -1.4
4,240,307 - BPHYT_RS18865 0.16 -4.4
4,240,307 - BPHYT_RS18865 0.16 -3.2
4,240,717 + BPHYT_RS18865 0.75 -2.7
4,240,875 - -2.4
4,240,909 + -2.4
4,240,930 + -3.7
4,240,930 + -4.2
4,240,930 + -1.3
4,240,931 - -2.4
4,241,000 - -3.7
4,241,065 + BPHYT_RS18870 0.17 -3.2
4,241,065 + BPHYT_RS18870 0.17 -4.7
4,241,066 - BPHYT_RS18870 0.17 -3.2
4,241,378 - BPHYT_RS18870 0.57 -4.2
4,241,476 + BPHYT_RS18870 0.69 -1.1
4,241,477 - BPHYT_RS18870 0.69 -2.3
4,241,582 - BPHYT_RS18870 0.83 -1.5
4,241,701 + -3.6
4,241,701 + -3.7
4,241,701 + -3.9
4,241,702 - -0.9
4,241,735 + -2.8
4,241,736 - -2.1
4,241,736 - -4.9
4,242,175 + BPHYT_RS18875 0.59 -3.0
4,242,176 - BPHYT_RS18875 0.59 -6.3
4,242,274 + BPHYT_RS18875 0.72 -1.9
4,242,274 + BPHYT_RS18875 0.72 -2.6
4,242,275 - BPHYT_RS18875 0.72 -2.2
4,242,511 - -1.1
4,242,511 - -2.1
4,242,574 - -3.7
4,242,574 - -2.6
4,242,574 - -2.3

Or see this region's nucleotide sequence