Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18855 and BPHYT_RS18860 are separated by 12 nucleotides BPHYT_RS18860 and BPHYT_RS18865 are separated by 2 nucleotides BPHYT_RS18865 and BPHYT_RS18870 are separated by 36 nucleotides
BPHYT_RS18855: BPHYT_RS18855 - flagellar rod assembly protein FlgJ, at 4,237,993 to 4,238,991
_RS18855
BPHYT_RS18860: BPHYT_RS18860 - flagellar P-ring protein FlgI, at 4,239,004 to 4,240,191
_RS18860
BPHYT_RS18865: BPHYT_RS18865 - flagellar L-ring protein FlgH, at 4,240,194 to 4,240,892
_RS18865
BPHYT_RS18870: BPHYT_RS18870 - flagellar basal body rod protein FlgG, at 4,240,929 to 4,241,717
_RS18870
Position (kb) 4239
4240
4241 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 4238.034 kb on - strand at 4238.148 kb on - strand, within BPHYT_RS18855 at 4238.169 kb on - strand, within BPHYT_RS18855 at 4238.223 kb on - strand, within BPHYT_RS18855 at 4238.223 kb on - strand, within BPHYT_RS18855 at 4238.223 kb on - strand, within BPHYT_RS18855 at 4238.422 kb on - strand, within BPHYT_RS18855 at 4238.496 kb on - strand, within BPHYT_RS18855 at 4238.653 kb on + strand, within BPHYT_RS18855 at 4238.654 kb on - strand, within BPHYT_RS18855 at 4238.980 kb on - strand at 4239.005 kb on + strand at 4239.005 kb on + strand at 4239.005 kb on + strand at 4239.005 kb on + strand at 4239.006 kb on - strand at 4239.006 kb on - strand at 4239.006 kb on - strand at 4239.088 kb on + strand at 4239.089 kb on - strand at 4239.089 kb on - strand at 4239.244 kb on - strand, within BPHYT_RS18860 at 4239.275 kb on - strand, within BPHYT_RS18860 at 4239.518 kb on + strand, within BPHYT_RS18860 at 4239.519 kb on - strand, within BPHYT_RS18860 at 4239.698 kb on + strand, within BPHYT_RS18860 at 4239.699 kb on - strand, within BPHYT_RS18860 at 4239.699 kb on - strand, within BPHYT_RS18860 at 4239.699 kb on - strand, within BPHYT_RS18860 at 4240.080 kb on - strand at 4240.185 kb on + strand at 4240.186 kb on - strand at 4240.238 kb on + strand at 4240.256 kb on + strand at 4240.258 kb on + strand at 4240.259 kb on - strand at 4240.259 kb on - strand at 4240.259 kb on - strand at 4240.307 kb on - strand, within BPHYT_RS18865 at 4240.307 kb on - strand, within BPHYT_RS18865 at 4240.717 kb on + strand, within BPHYT_RS18865 at 4240.875 kb on - strand at 4240.909 kb on + strand at 4240.930 kb on + strand at 4240.930 kb on + strand at 4240.930 kb on + strand at 4240.931 kb on - strand at 4241.000 kb on - strand at 4241.065 kb on + strand, within BPHYT_RS18870 at 4241.065 kb on + strand, within BPHYT_RS18870 at 4241.066 kb on - strand, within BPHYT_RS18870
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 4,238,034 - -5.1 4,238,148 - BPHYT_RS18855 0.16 -2.4 4,238,169 - BPHYT_RS18855 0.18 -3.6 4,238,223 - BPHYT_RS18855 0.23 -4.9 4,238,223 - BPHYT_RS18855 0.23 -2.6 4,238,223 - BPHYT_RS18855 0.23 -2.2 4,238,422 - BPHYT_RS18855 0.43 -4.0 4,238,496 - BPHYT_RS18855 0.50 -4.0 4,238,653 + BPHYT_RS18855 0.66 -3.7 4,238,654 - BPHYT_RS18855 0.66 -3.1 4,238,980 - -3.2 4,239,005 + -4.4 4,239,005 + -2.2 4,239,005 + -1.1 4,239,005 + -3.8 4,239,006 - -0.3 4,239,006 - -1.9 4,239,006 - +1.1 4,239,088 + -3.5 4,239,089 - -1.0 4,239,089 - -2.1 4,239,244 - BPHYT_RS18860 0.20 -3.9 4,239,275 - BPHYT_RS18860 0.23 -2.5 4,239,518 + BPHYT_RS18860 0.43 -3.1 4,239,519 - BPHYT_RS18860 0.43 -0.3 4,239,698 + BPHYT_RS18860 0.58 -1.8 4,239,699 - BPHYT_RS18860 0.59 -2.7 4,239,699 - BPHYT_RS18860 0.59 -2.3 4,239,699 - BPHYT_RS18860 0.59 -0.7 4,240,080 - -4.8 4,240,185 + -5.0 4,240,186 - -3.7 4,240,238 + -5.0 4,240,256 + -4.8 4,240,258 + -2.1 4,240,259 - -2.4 4,240,259 - -1.0 4,240,259 - -1.4 4,240,307 - BPHYT_RS18865 0.16 -4.4 4,240,307 - BPHYT_RS18865 0.16 -3.2 4,240,717 + BPHYT_RS18865 0.75 -2.7 4,240,875 - -2.4 4,240,909 + -2.4 4,240,930 + -3.7 4,240,930 + -4.2 4,240,930 + -1.3 4,240,931 - -2.4 4,241,000 - -3.7 4,241,065 + BPHYT_RS18870 0.17 -3.2 4,241,065 + BPHYT_RS18870 0.17 -4.7 4,241,066 - BPHYT_RS18870 0.17 -3.2
Or see this region's nucleotide sequence