Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18810 and BPHYT_RS18815 overlap by 8 nucleotides BPHYT_RS18815 and BPHYT_RS18820 are separated by 92 nucleotides BPHYT_RS18820 and BPHYT_RS18825 overlap by 1 nucleotides BPHYT_RS18825 and BPHYT_RS18830 are separated by 49 nucleotides
BPHYT_RS18810: BPHYT_RS18810 - flagellar motor switch protein FliM, at 4,229,273 to 4,230,271
_RS18810
BPHYT_RS18815: BPHYT_RS18815 - flagellar motor switch protein FliN, at 4,230,264 to 4,230,728
_RS18815
BPHYT_RS18820: BPHYT_RS18820 - flagellar biosynthesis protein FliO, at 4,230,821 to 4,231,336
_RS18820
BPHYT_RS18825: BPHYT_RS18825 - flagellar biosynthesis protein FliP, at 4,231,336 to 4,232,169
_RS18825
BPHYT_RS18830: BPHYT_RS18830 - flagellar biosynthesis protein FliQ, at 4,232,219 to 4,232,488
_RS18830
Position (kb) 4230
4231
4232 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 4229.917 kb on + strand, within BPHYT_RS18810 at 4229.917 kb on + strand, within BPHYT_RS18810 at 4229.918 kb on - strand, within BPHYT_RS18810 at 4229.918 kb on - strand, within BPHYT_RS18810 at 4229.918 kb on - strand, within BPHYT_RS18810 at 4229.918 kb on - strand, within BPHYT_RS18810 at 4229.918 kb on - strand, within BPHYT_RS18810 at 4230.207 kb on - strand at 4230.263 kb on + strand at 4230.264 kb on - strand at 4230.264 kb on - strand at 4230.692 kb on + strand at 4230.693 kb on - strand at 4230.824 kb on + strand at 4230.825 kb on - strand at 4231.115 kb on - strand, within BPHYT_RS18820 at 4231.284 kb on + strand, within BPHYT_RS18820 at 4231.284 kb on + strand, within BPHYT_RS18820 at 4231.547 kb on - strand, within BPHYT_RS18825 at 4231.759 kb on + strand, within BPHYT_RS18825 at 4231.760 kb on - strand, within BPHYT_RS18825 at 4231.761 kb on + strand, within BPHYT_RS18825 at 4231.762 kb on - strand, within BPHYT_RS18825 at 4231.762 kb on - strand, within BPHYT_RS18825 at 4231.772 kb on - strand, within BPHYT_RS18825 at 4232.083 kb on - strand, within BPHYT_RS18825 at 4232.261 kb on + strand, within BPHYT_RS18830 at 4232.261 kb on + strand, within BPHYT_RS18830 at 4232.261 kb on + strand, within BPHYT_RS18830 at 4232.261 kb on + strand, within BPHYT_RS18830 at 4232.261 kb on + strand, within BPHYT_RS18830 at 4232.261 kb on + strand, within BPHYT_RS18830 at 4232.261 kb on + strand, within BPHYT_RS18830 at 4232.261 kb on + strand at 4232.261 kb on + strand, within BPHYT_RS18830 at 4232.262 kb on - strand, within BPHYT_RS18830 at 4232.262 kb on - strand, within BPHYT_RS18830 at 4232.262 kb on - strand, within BPHYT_RS18830 at 4232.262 kb on - strand, within BPHYT_RS18830 at 4232.262 kb on - strand, within BPHYT_RS18830 at 4232.262 kb on - strand, within BPHYT_RS18830 at 4232.262 kb on - strand, within BPHYT_RS18830 at 4232.262 kb on - strand, within BPHYT_RS18830 at 4232.262 kb on - strand, within BPHYT_RS18830
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 4,229,917 + BPHYT_RS18810 0.64 -4.1 4,229,917 + BPHYT_RS18810 0.64 -0.6 4,229,918 - BPHYT_RS18810 0.65 -4.0 4,229,918 - BPHYT_RS18810 0.65 -2.1 4,229,918 - BPHYT_RS18810 0.65 -2.4 4,229,918 - BPHYT_RS18810 0.65 -3.4 4,229,918 - BPHYT_RS18810 0.65 -3.1 4,230,207 - -3.0 4,230,263 + -2.6 4,230,264 - -1.6 4,230,264 - -4.2 4,230,692 + -0.4 4,230,693 - -4.2 4,230,824 + -3.5 4,230,825 - -1.8 4,231,115 - BPHYT_RS18820 0.57 -2.7 4,231,284 + BPHYT_RS18820 0.90 +0.1 4,231,284 + BPHYT_RS18820 0.90 +0.6 4,231,547 - BPHYT_RS18825 0.25 -1.6 4,231,759 + BPHYT_RS18825 0.51 -3.1 4,231,760 - BPHYT_RS18825 0.51 -1.3 4,231,761 + BPHYT_RS18825 0.51 -2.3 4,231,762 - BPHYT_RS18825 0.51 -2.0 4,231,762 - BPHYT_RS18825 0.51 -1.6 4,231,772 - BPHYT_RS18825 0.52 -1.8 4,232,083 - BPHYT_RS18825 0.90 -3.9 4,232,261 + BPHYT_RS18830 0.16 -4.6 4,232,261 + BPHYT_RS18830 0.16 -3.7 4,232,261 + BPHYT_RS18830 0.16 -3.2 4,232,261 + BPHYT_RS18830 0.16 -2.6 4,232,261 + BPHYT_RS18830 0.16 -2.7 4,232,261 + BPHYT_RS18830 0.16 -5.7 4,232,261 + BPHYT_RS18830 0.16 -1.8 4,232,261 + -0.9 4,232,261 + BPHYT_RS18830 0.16 -0.3 4,232,262 - BPHYT_RS18830 0.16 -2.4 4,232,262 - BPHYT_RS18830 0.16 -1.9 4,232,262 - BPHYT_RS18830 0.16 -3.1 4,232,262 - BPHYT_RS18830 0.16 -1.6 4,232,262 - BPHYT_RS18830 0.16 -4.1 4,232,262 - BPHYT_RS18830 0.16 -2.5 4,232,262 - BPHYT_RS18830 0.16 -2.2 4,232,262 - BPHYT_RS18830 0.16 -3.7 4,232,262 - BPHYT_RS18830 0.16 -5.6
Or see this region's nucleotide sequence