Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS16135

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS16130 and BPHYT_RS16135 overlap by 4 nucleotidesBPHYT_RS16135 and BPHYT_RS16140 overlap by 8 nucleotidesBPHYT_RS16140 and BPHYT_RS16145 are separated by 58 nucleotides BPHYT_RS16130: BPHYT_RS16130 - cation ABC transporter substrate-binding protein, at 3,658,958 to 3,659,926 _RS16130 BPHYT_RS16135: BPHYT_RS16135 - ABC transporter ATP-binding protein, at 3,659,923 to 3,660,864 _RS16135 BPHYT_RS16140: BPHYT_RS16140 - ABC transporter permease, at 3,660,857 to 3,661,639 _RS16140 BPHYT_RS16145: BPHYT_RS16145 - S-formylglutathione hydrolase, at 3,661,698 to 3,662,549 _RS16145 Position (kb) 3659 3660 3661Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3659.243 kb on + strand, within BPHYT_RS16130at 3659.244 kb on - strand, within BPHYT_RS16130at 3659.369 kb on + strand, within BPHYT_RS16130at 3659.705 kb on + strand, within BPHYT_RS16130at 3659.705 kb on + strand, within BPHYT_RS16130at 3659.705 kb on + strand, within BPHYT_RS16130at 3659.705 kb on + strand, within BPHYT_RS16130at 3660.194 kb on - strand, within BPHYT_RS16135at 3660.195 kb on + strand, within BPHYT_RS16135at 3660.196 kb on - strand, within BPHYT_RS16135at 3660.377 kb on + strand, within BPHYT_RS16135at 3660.378 kb on - strand, within BPHYT_RS16135at 3660.593 kb on - strand, within BPHYT_RS16135at 3660.593 kb on - strand, within BPHYT_RS16135at 3660.593 kb on - strand, within BPHYT_RS16135at 3660.635 kb on + strand, within BPHYT_RS16135at 3660.636 kb on - strand, within BPHYT_RS16135at 3661.229 kb on - strand, within BPHYT_RS16140at 3661.559 kb on + strand, within BPHYT_RS16140at 3661.674 kb on - strandat 3661.759 kb on + strandat 3661.759 kb on + strandat 3661.760 kb on - strandat 3661.760 kb on - strandat 3661.763 kb on - strandat 3661.774 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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3,659,243 + BPHYT_RS16130 0.29 -2.1
3,659,244 - BPHYT_RS16130 0.30 -1.5
3,659,369 + BPHYT_RS16130 0.42 -1.6
3,659,705 + BPHYT_RS16130 0.77 -2.9
3,659,705 + BPHYT_RS16130 0.77 -0.5
3,659,705 + BPHYT_RS16130 0.77 -1.9
3,659,705 + BPHYT_RS16130 0.77 -4.2
3,660,194 - BPHYT_RS16135 0.29 -1.7
3,660,195 + BPHYT_RS16135 0.29 -1.3
3,660,196 - BPHYT_RS16135 0.29 -1.0
3,660,377 + BPHYT_RS16135 0.48 -0.2
3,660,378 - BPHYT_RS16135 0.48 -0.9
3,660,593 - BPHYT_RS16135 0.71 -1.9
3,660,593 - BPHYT_RS16135 0.71 -1.5
3,660,593 - BPHYT_RS16135 0.71 -1.8
3,660,635 + BPHYT_RS16135 0.76 -1.6
3,660,636 - BPHYT_RS16135 0.76 -0.6
3,661,229 - BPHYT_RS16140 0.48 -1.5
3,661,559 + BPHYT_RS16140 0.90 -0.3
3,661,674 - -0.5
3,661,759 + -2.3
3,661,759 + +0.4
3,661,760 - -0.6
3,661,760 - -0.2
3,661,763 - -0.5
3,661,774 + -3.0

Or see this region's nucleotide sequence