Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15570 and BPHYT_RS15575 are separated by 109 nucleotides BPHYT_RS15575 and BPHYT_RS15580 are separated by 360 nucleotides
BPHYT_RS15570: BPHYT_RS15570 - NAD-dependent dehydratase, at 3,532,615 to 3,533,640
_RS15570
BPHYT_RS15575: BPHYT_RS15575 - CDP-6-deoxy-delta-3,4-glucoseen reductase, at 3,533,750 to 3,534,781
_RS15575
BPHYT_RS15580: BPHYT_RS15580 - acetylornithine aminotransferase, at 3,535,142 to 3,536,326
_RS15580
Position (kb)
3533
3534
3535 Strain fitness (log2 ratio)
-2
-1
0
1 at 3532.821 kb on - strand, within BPHYT_RS15570 at 3532.861 kb on + strand, within BPHYT_RS15570 at 3532.862 kb on - strand, within BPHYT_RS15570 at 3532.862 kb on - strand, within BPHYT_RS15570 at 3533.009 kb on + strand, within BPHYT_RS15570 at 3533.009 kb on + strand, within BPHYT_RS15570 at 3533.010 kb on - strand, within BPHYT_RS15570 at 3533.010 kb on - strand, within BPHYT_RS15570 at 3533.175 kb on + strand, within BPHYT_RS15570 at 3533.185 kb on + strand, within BPHYT_RS15570 at 3533.241 kb on + strand, within BPHYT_RS15570 at 3533.242 kb on - strand, within BPHYT_RS15570 at 3533.242 kb on - strand, within BPHYT_RS15570 at 3533.242 kb on - strand, within BPHYT_RS15570 at 3533.596 kb on + strand at 3533.602 kb on + strand at 3533.603 kb on - strand at 3533.603 kb on - strand at 3533.654 kb on + strand at 3533.655 kb on - strand at 3533.758 kb on + strand at 3533.758 kb on + strand at 3533.759 kb on - strand at 3533.759 kb on - strand at 3533.759 kb on - strand at 3533.796 kb on + strand at 3534.143 kb on + strand, within BPHYT_RS15575 at 3534.197 kb on + strand, within BPHYT_RS15575 at 3534.289 kb on + strand, within BPHYT_RS15575 at 3534.491 kb on + strand, within BPHYT_RS15575 at 3534.491 kb on + strand, within BPHYT_RS15575 at 3534.492 kb on - strand, within BPHYT_RS15575 at 3534.644 kb on + strand, within BPHYT_RS15575 at 3534.653 kb on + strand, within BPHYT_RS15575 at 3534.653 kb on + strand, within BPHYT_RS15575 at 3534.653 kb on + strand, within BPHYT_RS15575 at 3534.654 kb on - strand, within BPHYT_RS15575 at 3534.734 kb on + strand at 3534.735 kb on - strand at 3534.735 kb on - strand at 3534.824 kb on - strand at 3534.828 kb on - strand at 3535.178 kb on + strand at 3535.367 kb on + strand, within BPHYT_RS15580 at 3535.368 kb on - strand, within BPHYT_RS15580 at 3535.411 kb on + strand, within BPHYT_RS15580 at 3535.411 kb on + strand, within BPHYT_RS15580 at 3535.411 kb on + strand, within BPHYT_RS15580 at 3535.412 kb on - strand, within BPHYT_RS15580 at 3535.601 kb on + strand, within BPHYT_RS15580 at 3535.601 kb on + strand, within BPHYT_RS15580 at 3535.602 kb on - strand, within BPHYT_RS15580
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,532,821 - BPHYT_RS15570 0.20 +1.1 3,532,861 + BPHYT_RS15570 0.24 +0.3 3,532,862 - BPHYT_RS15570 0.24 +0.4 3,532,862 - BPHYT_RS15570 0.24 +0.7 3,533,009 + BPHYT_RS15570 0.38 -1.2 3,533,009 + BPHYT_RS15570 0.38 -0.1 3,533,010 - BPHYT_RS15570 0.38 -1.1 3,533,010 - BPHYT_RS15570 0.38 -1.3 3,533,175 + BPHYT_RS15570 0.55 -0.3 3,533,185 + BPHYT_RS15570 0.56 -0.5 3,533,241 + BPHYT_RS15570 0.61 -1.6 3,533,242 - BPHYT_RS15570 0.61 +0.1 3,533,242 - BPHYT_RS15570 0.61 -0.2 3,533,242 - BPHYT_RS15570 0.61 +1.0 3,533,596 + +1.0 3,533,602 + -1.3 3,533,603 - +0.5 3,533,603 - -0.3 3,533,654 + +0.2 3,533,655 - +0.2 3,533,758 + -0.5 3,533,758 + -1.5 3,533,759 - -2.1 3,533,759 - -0.6 3,533,759 - -1.0 3,533,796 + -0.8 3,534,143 + BPHYT_RS15575 0.38 -1.6 3,534,197 + BPHYT_RS15575 0.43 -1.5 3,534,289 + BPHYT_RS15575 0.52 -0.2 3,534,491 + BPHYT_RS15575 0.72 -1.8 3,534,491 + BPHYT_RS15575 0.72 -0.2 3,534,492 - BPHYT_RS15575 0.72 -0.4 3,534,644 + BPHYT_RS15575 0.87 -1.5 3,534,653 + BPHYT_RS15575 0.88 -1.1 3,534,653 + BPHYT_RS15575 0.88 -0.5 3,534,653 + BPHYT_RS15575 0.88 -0.6 3,534,654 - BPHYT_RS15575 0.88 -1.0 3,534,734 + +0.1 3,534,735 - -0.7 3,534,735 - +1.3 3,534,824 - -0.2 3,534,828 - -0.4 3,535,178 + -0.0 3,535,367 + BPHYT_RS15580 0.19 -0.1 3,535,368 - BPHYT_RS15580 0.19 -1.8 3,535,411 + BPHYT_RS15580 0.23 +0.4 3,535,411 + BPHYT_RS15580 0.23 +0.6 3,535,411 + BPHYT_RS15580 0.23 -0.4 3,535,412 - BPHYT_RS15580 0.23 -1.3 3,535,601 + BPHYT_RS15580 0.39 +0.2 3,535,601 + BPHYT_RS15580 0.39 -0.4 3,535,602 - BPHYT_RS15580 0.39 -0.2
Or see this region's nucleotide sequence