Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS14395

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS14390 and BPHYT_RS14395 are separated by 35 nucleotidesBPHYT_RS14395 and BPHYT_RS14400 are separated by 4 nucleotidesBPHYT_RS14400 and BPHYT_RS14405 are separated by 83 nucleotides BPHYT_RS14390: BPHYT_RS14390 - peptidase, at 3,284,234 to 3,285,751 _RS14390 BPHYT_RS14395: BPHYT_RS14395 - sugar dehydratase, at 3,285,787 to 3,286,830 _RS14395 BPHYT_RS14400: BPHYT_RS14400 - anti-sigma factor, at 3,286,835 to 3,287,464 _RS14400 BPHYT_RS14405: BPHYT_RS14405 - RNA polymerase sigma factor RpoE, at 3,287,548 to 3,288,147 _RS14405 Position (kb) 3285 3286 3287Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2at 3285.002 kb on - strand, within BPHYT_RS14390at 3285.066 kb on + strand, within BPHYT_RS14390at 3285.066 kb on + strand, within BPHYT_RS14390at 3285.066 kb on + strand, within BPHYT_RS14390at 3285.298 kb on - strand, within BPHYT_RS14390at 3285.589 kb on + strand, within BPHYT_RS14390at 3285.707 kb on + strandat 3285.752 kb on - strandat 3285.752 kb on - strandat 3285.804 kb on - strandat 3285.804 kb on - strandat 3285.804 kb on - strandat 3285.804 kb on - strandat 3285.804 kb on - strandat 3285.809 kb on - strandat 3286.158 kb on + strand, within BPHYT_RS14395at 3286.159 kb on - strand, within BPHYT_RS14395at 3286.165 kb on - strand, within BPHYT_RS14395at 3286.304 kb on + strand, within BPHYT_RS14395at 3286.305 kb on - strand, within BPHYT_RS14395at 3286.386 kb on + strand, within BPHYT_RS14395at 3286.387 kb on - strand, within BPHYT_RS14395at 3286.387 kb on - strand, within BPHYT_RS14395at 3286.499 kb on + strand, within BPHYT_RS14395at 3286.746 kb on + strandat 3286.801 kb on - strandat 3286.836 kb on + strandat 3286.836 kb on + strandat 3286.930 kb on - strand, within BPHYT_RS14400at 3287.314 kb on - strand, within BPHYT_RS14400at 3287.314 kb on - strand, within BPHYT_RS14400at 3287.357 kb on - strand, within BPHYT_RS14400at 3287.479 kb on + strandat 3287.479 kb on + strandat 3287.480 kb on - strandat 3287.480 kb on - strandat 3287.485 kb on + strandat 3287.485 kb on + strandat 3287.486 kb on - strandat 3287.514 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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3,285,002 - BPHYT_RS14390 0.51 -0.9
3,285,066 + BPHYT_RS14390 0.55 -1.3
3,285,066 + BPHYT_RS14390 0.55 -1.2
3,285,066 + BPHYT_RS14390 0.55 -0.6
3,285,298 - BPHYT_RS14390 0.70 +1.8
3,285,589 + BPHYT_RS14390 0.89 -1.3
3,285,707 + -2.0
3,285,752 - +0.4
3,285,752 - +0.1
3,285,804 - -1.0
3,285,804 - -0.2
3,285,804 - -0.8
3,285,804 - -0.3
3,285,804 - -0.8
3,285,809 - -1.2
3,286,158 + BPHYT_RS14395 0.36 -2.4
3,286,159 - BPHYT_RS14395 0.36 -1.9
3,286,165 - BPHYT_RS14395 0.36 -3.4
3,286,304 + BPHYT_RS14395 0.50 -2.7
3,286,305 - BPHYT_RS14395 0.50 -2.6
3,286,386 + BPHYT_RS14395 0.57 -3.1
3,286,387 - BPHYT_RS14395 0.57 -4.6
3,286,387 - BPHYT_RS14395 0.57 -5.0
3,286,499 + BPHYT_RS14395 0.68 -0.3
3,286,746 + -4.8
3,286,801 - -3.3
3,286,836 + -6.0
3,286,836 + -3.5
3,286,930 - BPHYT_RS14400 0.15 -1.1
3,287,314 - BPHYT_RS14400 0.76 -5.4
3,287,314 - BPHYT_RS14400 0.76 -4.1
3,287,357 - BPHYT_RS14400 0.83 -2.1
3,287,479 + -3.4
3,287,479 + -3.8
3,287,480 - -1.2
3,287,480 - -1.4
3,287,485 + -3.8
3,287,485 + -5.4
3,287,486 - -3.0
3,287,514 - +0.3

Or see this region's nucleotide sequence