Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS13275

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS13265 and BPHYT_RS13270 are separated by 315 nucleotidesBPHYT_RS13270 and BPHYT_RS13275 are separated by 266 nucleotidesBPHYT_RS13275 and BPHYT_RS13280 are separated by 278 nucleotides BPHYT_RS13265: BPHYT_RS13265 - hypothetical protein, at 3,028,619 to 3,029,107 _RS13265 BPHYT_RS13270: BPHYT_RS13270 - hypothetical protein, at 3,029,423 to 3,029,752 _RS13270 BPHYT_RS13275: BPHYT_RS13275 - methyltransferase, at 3,030,019 to 3,030,915 _RS13275 BPHYT_RS13280: BPHYT_RS13280 - 2-isopropylmalate synthase, at 3,031,194 to 3,032,912 _RS13280 Position (kb) 3030 3031Strain fitness (log2 ratio) -2 -1 0 1 2at 3029.034 kb on + strand, within BPHYT_RS13265at 3029.141 kb on - strandat 3029.187 kb on + strandat 3029.348 kb on + strandat 3029.348 kb on + strandat 3029.451 kb on + strandat 3029.452 kb on - strandat 3029.752 kb on + strandat 3029.752 kb on + strandat 3029.845 kb on + strandat 3029.845 kb on + strandat 3029.845 kb on + strandat 3029.846 kb on - strandat 3029.847 kb on + strandat 3029.928 kb on - strandat 3029.976 kb on + strandat 3029.977 kb on - strandat 3030.317 kb on - strand, within BPHYT_RS13275at 3030.374 kb on - strand, within BPHYT_RS13275at 3030.374 kb on - strand, within BPHYT_RS13275at 3030.460 kb on + strand, within BPHYT_RS13275at 3030.694 kb on + strand, within BPHYT_RS13275at 3030.695 kb on - strand, within BPHYT_RS13275at 3030.968 kb on + strandat 3030.968 kb on + strandat 3030.976 kb on + strandat 3030.987 kb on - strandat 3030.987 kb on - strandat 3030.990 kb on + strandat 3031.198 kb on + strandat 3031.212 kb on - strandat 3031.218 kb on + strandat 3031.219 kb on - strandat 3031.575 kb on + strand, within BPHYT_RS13280at 3031.576 kb on - strand, within BPHYT_RS13280at 3031.713 kb on + strand, within BPHYT_RS13280at 3031.714 kb on - strand, within BPHYT_RS13280at 3031.857 kb on + strand, within BPHYT_RS13280

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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3,029,034 + BPHYT_RS13265 0.85 +0.3
3,029,141 - -0.2
3,029,187 + -2.5
3,029,348 + -0.3
3,029,348 + -0.1
3,029,451 + -0.1
3,029,452 - -0.6
3,029,752 + +0.1
3,029,752 + -0.7
3,029,845 + -0.8
3,029,845 + -0.6
3,029,845 + -0.2
3,029,846 - -1.4
3,029,847 + -1.8
3,029,928 - -0.1
3,029,976 + -1.2
3,029,977 - -0.3
3,030,317 - BPHYT_RS13275 0.33 +0.5
3,030,374 - BPHYT_RS13275 0.40 +0.4
3,030,374 - BPHYT_RS13275 0.40 +1.9
3,030,460 + BPHYT_RS13275 0.49 +1.2
3,030,694 + BPHYT_RS13275 0.75 +0.4
3,030,695 - BPHYT_RS13275 0.75 +0.7
3,030,968 + -0.6
3,030,968 + -0.1
3,030,976 + +0.7
3,030,987 - -0.0
3,030,987 - +0.5
3,030,990 + -0.6
3,031,198 + +1.2
3,031,212 - +0.1
3,031,218 + +0.1
3,031,219 - -0.2
3,031,575 + BPHYT_RS13280 0.22 +0.9
3,031,576 - BPHYT_RS13280 0.22 -0.6
3,031,713 + BPHYT_RS13280 0.30 -0.1
3,031,714 - BPHYT_RS13280 0.30 +1.8
3,031,857 + BPHYT_RS13280 0.39 -0.2

Or see this region's nucleotide sequence