Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS11855

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS11850 and BPHYT_RS11855 are separated by 86 nucleotidesBPHYT_RS11855 and BPHYT_RS11860 are separated by 79 nucleotides BPHYT_RS11850: BPHYT_RS11850 - transcriptional regulator, at 2,686,636 to 2,687,550 _RS11850 BPHYT_RS11855: BPHYT_RS11855 - flagellar motor protein MotB, at 2,687,637 to 2,688,359 _RS11855 BPHYT_RS11860: BPHYT_RS11860 - hypothetical protein, at 2,688,439 to 2,694,864 _RS11860 Position (kb) 2687 2688 2689Strain fitness (log2 ratio) -2 -1 0 1 2at 2687.405 kb on - strand, within BPHYT_RS11850at 2687.792 kb on - strand, within BPHYT_RS11855at 2687.831 kb on - strand, within BPHYT_RS11855at 2687.831 kb on - strand, within BPHYT_RS11855at 2688.194 kb on + strand, within BPHYT_RS11855at 2688.194 kb on + strand, within BPHYT_RS11855at 2688.270 kb on - strand, within BPHYT_RS11855at 2688.657 kb on - strandat 2688.716 kb on + strandat 2688.861 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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2,687,405 - BPHYT_RS11850 0.84 +0.6
2,687,792 - BPHYT_RS11855 0.21 +2.0
2,687,831 - BPHYT_RS11855 0.27 -0.9
2,687,831 - BPHYT_RS11855 0.27 +0.1
2,688,194 + BPHYT_RS11855 0.77 +0.4
2,688,194 + BPHYT_RS11855 0.77 -0.0
2,688,270 - BPHYT_RS11855 0.88 +0.9
2,688,657 - -1.8
2,688,716 + +0.6
2,688,861 + -1.3

Or see this region's nucleotide sequence