Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS11085 and BPHYT_RS11090 are separated by 10 nucleotides BPHYT_RS11090 and BPHYT_RS11095 are separated by 35 nucleotides BPHYT_RS11095 and BPHYT_RS11100 overlap by 4 nucleotides BPHYT_RS11100 and BPHYT_RS11105 are separated by 24 nucleotides
BPHYT_RS11085: BPHYT_RS11085 - major facilitator transporter, at 2,519,848 to 2,521,245
_RS11085
BPHYT_RS11090: BPHYT_RS11090 - membrane protein, at 2,521,256 to 2,521,747
_RS11090
BPHYT_RS11095: BPHYT_RS11095 - glycosyl transferase, at 2,521,783 to 2,522,439
_RS11095
BPHYT_RS11100: BPHYT_RS11100 - methyltransferase, at 2,522,436 to 2,523,041
_RS11100
BPHYT_RS11105: BPHYT_RS11105 - acyl-CoA dehydrogenase, at 2,523,066 to 2,524,244
_RS11105
Position (kb) 2521
2522
2523 Strain fitness (log2 ratio) -1
0
1 at 2521.727 kb on + strand at 2521.970 kb on + strand, within BPHYT_RS11095 at 2522.033 kb on + strand, within BPHYT_RS11095 at 2522.439 kb on + strand at 2522.439 kb on + strand at 2522.687 kb on - strand, within BPHYT_RS11100 at 2523.022 kb on + strand at 2523.251 kb on + strand, within BPHYT_RS11105 at 2523.311 kb on + strand, within BPHYT_RS11105
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,521,727 + +1.0 2,521,970 + BPHYT_RS11095 0.28 +1.1 2,522,033 + BPHYT_RS11095 0.38 +0.5 2,522,439 + -1.5 2,522,439 + -0.1 2,522,687 - BPHYT_RS11100 0.41 -0.4 2,523,022 + -0.9 2,523,251 + BPHYT_RS11105 0.16 -0.1 2,523,311 + BPHYT_RS11105 0.21 -0.3
Or see this region's nucleotide sequence