Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS09725

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS09715 and BPHYT_RS09720 are separated by 43 nucleotidesBPHYT_RS09720 and BPHYT_RS09725 overlap by 7 nucleotidesBPHYT_RS09725 and BPHYT_RS09730 are separated by 135 nucleotides BPHYT_RS09715: BPHYT_RS09715 - UDP-glucose 6-dehydrogenase, at 2,202,236 to 2,203,684 _RS09715 BPHYT_RS09720: BPHYT_RS09720 - exopolysaccharide biosynthesis protein, at 2,203,728 to 2,204,183 _RS09720 BPHYT_RS09725: BPHYT_RS09725 - exopolysaccharide biosynthesis protein, at 2,204,177 to 2,205,406 _RS09725 BPHYT_RS09730: BPHYT_RS09730 - exopolysaccharide biosynthesis protein, at 2,205,542 to 2,207,740 _RS09730 Position (kb) 2204 2205 2206Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2204.312 kb on - strand, within BPHYT_RS09725at 2204.469 kb on - strand, within BPHYT_RS09725at 2204.475 kb on - strand, within BPHYT_RS09725at 2204.756 kb on + strand, within BPHYT_RS09725at 2205.031 kb on - strand, within BPHYT_RS09725at 2205.233 kb on + strand, within BPHYT_RS09725at 2205.309 kb on - strandat 2205.690 kb on - strandat 2205.968 kb on + strand, within BPHYT_RS09730at 2206.077 kb on - strand, within BPHYT_RS09730at 2206.343 kb on + strand, within BPHYT_RS09730at 2206.343 kb on + strand, within BPHYT_RS09730at 2206.344 kb on - strand, within BPHYT_RS09730

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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2,204,312 - BPHYT_RS09725 0.11 -0.4
2,204,469 - BPHYT_RS09725 0.24 -1.2
2,204,475 - BPHYT_RS09725 0.24 +2.5
2,204,756 + BPHYT_RS09725 0.47 +0.9
2,205,031 - BPHYT_RS09725 0.69 +0.4
2,205,233 + BPHYT_RS09725 0.86 -2.9
2,205,309 - +0.5
2,205,690 - +1.6
2,205,968 + BPHYT_RS09730 0.19 -0.9
2,206,077 - BPHYT_RS09730 0.24 +1.0
2,206,343 + BPHYT_RS09730 0.36 +0.4
2,206,343 + BPHYT_RS09730 0.36 +0.1
2,206,344 - BPHYT_RS09730 0.36 -0.3

Or see this region's nucleotide sequence