Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09390 and BPHYT_RS09395 are separated by 16 nucleotides BPHYT_RS09395 and BPHYT_RS09400 overlap by 14 nucleotides BPHYT_RS09400 and BPHYT_RS09405 are separated by 57 nucleotides
BPHYT_RS09390: BPHYT_RS09390 - ABC transporter permease, at 2,126,771 to 2,127,568
_RS09390
BPHYT_RS09395: BPHYT_RS09395 - ABC transporter permease, at 2,127,585 to 2,128,457
_RS09395
BPHYT_RS09400: BPHYT_RS09400 - spermidine/putrescine ABC transporter ATPase, at 2,128,444 to 2,129,523
_RS09400
BPHYT_RS09405: BPHYT_RS09405 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,129,581 to 2,130,648
_RS09405
Position (kb)
2128
2129
2130 Strain fitness (log2 ratio)
-1
0
1
2 at 2127.523 kb on + strand at 2127.523 kb on + strand at 2127.537 kb on + strand at 2127.718 kb on + strand, within BPHYT_RS09395 at 2128.261 kb on + strand, within BPHYT_RS09395 at 2128.262 kb on - strand, within BPHYT_RS09395 at 2128.694 kb on + strand, within BPHYT_RS09400 at 2128.860 kb on - strand, within BPHYT_RS09400 at 2128.860 kb on - strand at 2128.868 kb on + strand, within BPHYT_RS09400 at 2128.868 kb on + strand, within BPHYT_RS09400 at 2129.062 kb on + strand, within BPHYT_RS09400 at 2129.062 kb on + strand, within BPHYT_RS09400 at 2129.063 kb on - strand, within BPHYT_RS09400 at 2129.273 kb on + strand, within BPHYT_RS09400 at 2129.273 kb on + strand, within BPHYT_RS09400 at 2129.274 kb on - strand, within BPHYT_RS09400 at 2129.770 kb on + strand, within BPHYT_RS09405 at 2129.770 kb on + strand, within BPHYT_RS09405 at 2129.771 kb on - strand, within BPHYT_RS09405 at 2129.771 kb on - strand, within BPHYT_RS09405 at 2129.789 kb on + strand, within BPHYT_RS09405 at 2130.110 kb on + strand, within BPHYT_RS09405 at 2130.111 kb on - strand, within BPHYT_RS09405 at 2130.347 kb on + strand, within BPHYT_RS09405 at 2130.421 kb on - strand, within BPHYT_RS09405
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,127,523 + +1.1 2,127,523 + +1.4 2,127,537 + +0.0 2,127,718 + BPHYT_RS09395 0.15 +0.3 2,128,261 + BPHYT_RS09395 0.77 -0.5 2,128,262 - BPHYT_RS09395 0.78 -0.4 2,128,694 + BPHYT_RS09400 0.23 +0.4 2,128,860 - BPHYT_RS09400 0.39 +0.1 2,128,860 - +0.7 2,128,868 + BPHYT_RS09400 0.39 +0.4 2,128,868 + BPHYT_RS09400 0.39 +0.4 2,129,062 + BPHYT_RS09400 0.57 +1.5 2,129,062 + BPHYT_RS09400 0.57 +0.3 2,129,063 - BPHYT_RS09400 0.57 -0.1 2,129,273 + BPHYT_RS09400 0.77 +0.4 2,129,273 + BPHYT_RS09400 0.77 +1.2 2,129,274 - BPHYT_RS09400 0.77 +0.2 2,129,770 + BPHYT_RS09405 0.18 +0.4 2,129,770 + BPHYT_RS09405 0.18 +0.3 2,129,771 - BPHYT_RS09405 0.18 +0.3 2,129,771 - BPHYT_RS09405 0.18 -0.2 2,129,789 + BPHYT_RS09405 0.19 +0.3 2,130,110 + BPHYT_RS09405 0.50 -0.7 2,130,111 - BPHYT_RS09405 0.50 -0.0 2,130,347 + BPHYT_RS09405 0.72 +0.9 2,130,421 - BPHYT_RS09405 0.79 +2.0
Or see this region's nucleotide sequence