Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02970 and BPHYT_RS02975 are separated by 386 nucleotides BPHYT_RS02975 and BPHYT_RS02980 are separated by 135 nucleotides BPHYT_RS02980 and BPHYT_RS02985 are separated by 296 nucleotides
BPHYT_RS02970: BPHYT_RS02970 - excinuclease ABC subunit A, at 659,698 to 662,571
_RS02970
BPHYT_RS02975: BPHYT_RS02975 - membrane protein, at 662,958 to 664,115
_RS02975
BPHYT_RS02980: BPHYT_RS02980 - single-stranded DNA-binding protein, at 664,251 to 664,790
_RS02980
BPHYT_RS02985: BPHYT_RS02985 - MaoC family dehydratase, at 665,087 to 665,587
_RS02985
Position (kb) 662
663
664
665 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 661.991 kb on + strand, within BPHYT_RS02970 at 661.991 kb on + strand, within BPHYT_RS02970 at 661.992 kb on - strand, within BPHYT_RS02970 at 661.992 kb on - strand, within BPHYT_RS02970 at 661.992 kb on - strand, within BPHYT_RS02970 at 661.992 kb on - strand, within BPHYT_RS02970 at 662.217 kb on - strand, within BPHYT_RS02970 at 662.252 kb on + strand, within BPHYT_RS02970 at 662.252 kb on + strand, within BPHYT_RS02970 at 662.253 kb on - strand, within BPHYT_RS02970 at 662.253 kb on - strand, within BPHYT_RS02970 at 662.394 kb on - strand at 662.414 kb on + strand at 662.415 kb on - strand at 662.415 kb on - strand at 662.510 kb on - strand at 662.610 kb on - strand at 662.618 kb on + strand at 662.618 kb on + strand at 662.618 kb on + strand at 662.618 kb on + strand at 662.621 kb on + strand at 662.621 kb on + strand at 662.621 kb on + strand at 662.621 kb on + strand at 662.621 kb on + strand at 662.687 kb on + strand at 662.745 kb on - strand at 663.147 kb on + strand, within BPHYT_RS02975 at 663.619 kb on + strand, within BPHYT_RS02975 at 663.620 kb on - strand, within BPHYT_RS02975 at 663.684 kb on + strand, within BPHYT_RS02975 at 663.954 kb on + strand, within BPHYT_RS02975 at 664.074 kb on - strand at 664.074 kb on - strand at 664.075 kb on + strand at 664.947 kb on + strand at 665.019 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 661,991 + BPHYT_RS02970 0.80 +0.5 661,991 + BPHYT_RS02970 0.80 +0.4 661,992 - BPHYT_RS02970 0.80 +0.5 661,992 - BPHYT_RS02970 0.80 +1.3 661,992 - BPHYT_RS02970 0.80 +0.4 661,992 - BPHYT_RS02970 0.80 -0.4 662,217 - BPHYT_RS02970 0.88 +0.6 662,252 + BPHYT_RS02970 0.89 +0.7 662,252 + BPHYT_RS02970 0.89 +0.6 662,253 - BPHYT_RS02970 0.89 +0.6 662,253 - BPHYT_RS02970 0.89 +0.3 662,394 - -0.9 662,414 + +0.4 662,415 - +0.8 662,415 - +0.8 662,510 - +0.4 662,610 - -1.9 662,618 + +0.8 662,618 + +0.8 662,618 + +0.8 662,618 + +1.3 662,621 + +1.1 662,621 + +1.9 662,621 + +0.8 662,621 + -0.9 662,621 + +0.4 662,687 + +0.1 662,745 - -1.5 663,147 + BPHYT_RS02975 0.16 -2.2 663,619 + BPHYT_RS02975 0.57 -0.0 663,620 - BPHYT_RS02975 0.57 +1.7 663,684 + BPHYT_RS02975 0.63 -3.1 663,954 + BPHYT_RS02975 0.86 -2.3 664,074 - -1.8 664,074 - -0.3 664,075 + -0.2 664,947 + -0.1 665,019 + +0.7
Or see this region's nucleotide sequence