Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS02180

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS02175 and BPHYT_RS02180 are separated by 308 nucleotidesBPHYT_RS02180 and BPHYT_RS02185 are separated by 280 nucleotides BPHYT_RS02175: BPHYT_RS02175 - type VI secretion protein ImpA, at 499,197 to 501,539 _RS02175 BPHYT_RS02180: BPHYT_RS02180 - hypothetical protein, at 501,848 to 502,372 _RS02180 BPHYT_RS02185: BPHYT_RS02185 - hypothetical protein, at 502,653 to 503,165 _RS02185 Position (kb) 501 502 503Strain fitness (log2 ratio) -2 -1 0 1at 500.988 kb on - strand, within BPHYT_RS02175at 501.572 kb on - strandat 501.667 kb on + strandat 501.667 kb on + strandat 501.746 kb on - strandat 502.019 kb on - strand, within BPHYT_RS02180at 502.178 kb on + strand, within BPHYT_RS02180at 502.261 kb on + strand, within BPHYT_RS02180at 502.410 kb on - strandat 502.516 kb on + strandat 502.680 kb on - strandat 502.847 kb on + strand, within BPHYT_RS02185at 502.848 kb on - strand, within BPHYT_RS02185at 503.100 kb on + strand, within BPHYT_RS02185at 503.178 kb on + strandat 503.187 kb on + strandat 503.188 kb on - strandat 503.278 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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500,988 - BPHYT_RS02175 0.76 -0.1
501,572 - -0.5
501,667 + -0.7
501,667 + -0.2
501,746 - -0.2
502,019 - BPHYT_RS02180 0.33 -1.8
502,178 + BPHYT_RS02180 0.63 -2.7
502,261 + BPHYT_RS02180 0.79 -0.5
502,410 - -0.3
502,516 + -0.8
502,680 - +0.3
502,847 + BPHYT_RS02185 0.38 -1.2
502,848 - BPHYT_RS02185 0.38 -0.1
503,100 + BPHYT_RS02185 0.87 -0.6
503,178 + +0.5
503,187 + -0.9
503,188 - -0.6
503,278 - -1.3

Or see this region's nucleotide sequence