Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS01425

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS01420 and BPHYT_RS01425 are separated by 107 nucleotidesBPHYT_RS01425 and BPHYT_RS01430 are separated by 376 nucleotides BPHYT_RS01420: BPHYT_RS01420 - patatin, at 322,482 to 323,360 _RS01420 BPHYT_RS01425: BPHYT_RS01425 - 3-phosphoglycerate dehydrogenase, at 323,468 to 324,481 _RS01425 BPHYT_RS01430: BPHYT_RS01430 - phosphoenolpyruvate carboxykinase, at 324,858 to 326,714 _RS01430 Position (kb) 323 324 325Strain fitness (log2 ratio) -3 -2 -1 0 1at 322.499 kb on - strandat 322.499 kb on - strandat 322.593 kb on - strand, within BPHYT_RS01420at 322.626 kb on + strand, within BPHYT_RS01420at 322.626 kb on + strand, within BPHYT_RS01420at 322.627 kb on - strand, within BPHYT_RS01420at 322.627 kb on - strand, within BPHYT_RS01420at 322.656 kb on + strand, within BPHYT_RS01420at 322.656 kb on + strand, within BPHYT_RS01420at 322.659 kb on + strand, within BPHYT_RS01420at 322.659 kb on + strand, within BPHYT_RS01420at 322.659 kb on + strand, within BPHYT_RS01420at 322.659 kb on + strand, within BPHYT_RS01420at 322.659 kb on + strand, within BPHYT_RS01420at 322.660 kb on - strand, within BPHYT_RS01420at 322.737 kb on + strand, within BPHYT_RS01420at 322.746 kb on + strand, within BPHYT_RS01420at 322.771 kb on - strand, within BPHYT_RS01420at 322.882 kb on - strand, within BPHYT_RS01420at 322.882 kb on - strand, within BPHYT_RS01420at 322.896 kb on + strand, within BPHYT_RS01420at 322.896 kb on + strand, within BPHYT_RS01420at 323.152 kb on - strand, within BPHYT_RS01420at 323.202 kb on + strand, within BPHYT_RS01420at 323.202 kb on + strand, within BPHYT_RS01420at 323.202 kb on + strand, within BPHYT_RS01420at 323.274 kb on + strandat 323.274 kb on + strandat 323.274 kb on + strandat 323.275 kb on - strandat 323.443 kb on - strandat 323.451 kb on - strandat 323.565 kb on + strandat 323.641 kb on - strand, within BPHYT_RS01425at 323.846 kb on + strand, within BPHYT_RS01425at 323.846 kb on + strand, within BPHYT_RS01425at 324.030 kb on - strand, within BPHYT_RS01425at 324.100 kb on + strand, within BPHYT_RS01425at 324.175 kb on - strand, within BPHYT_RS01425at 324.206 kb on - strand, within BPHYT_RS01425at 324.484 kb on + strandat 324.484 kb on + strandat 324.484 kb on + strandat 324.485 kb on - strandat 324.485 kb on - strandat 324.485 kb on - strandat 324.527 kb on - strandat 324.553 kb on - strandat 324.588 kb on - strandat 324.591 kb on - strandat 324.591 kb on - strandat 324.595 kb on - strandat 324.643 kb on - strandat 324.676 kb on - strandat 324.831 kb on - strandat 325.002 kb on + strandat 325.078 kb on - strand, within BPHYT_RS01430at 325.169 kb on + strand, within BPHYT_RS01430at 325.169 kb on + strand, within BPHYT_RS01430at 325.169 kb on + strand, within BPHYT_RS01430at 325.170 kb on - strand, within BPHYT_RS01430at 325.245 kb on + strand, within BPHYT_RS01430at 325.245 kb on + strand, within BPHYT_RS01430at 325.245 kb on + strand, within BPHYT_RS01430at 325.245 kb on + strand, within BPHYT_RS01430at 325.245 kb on + strand, within BPHYT_RS01430at 325.245 kb on + strand, within BPHYT_RS01430at 325.245 kb on + strand, within BPHYT_RS01430at 325.245 kb on + strand, within BPHYT_RS01430at 325.246 kb on - strand, within BPHYT_RS01430at 325.246 kb on - strand, within BPHYT_RS01430at 325.246 kb on - strand, within BPHYT_RS01430at 325.246 kb on - strand, within BPHYT_RS01430at 325.246 kb on - strand, within BPHYT_RS01430at 325.246 kb on - strand, within BPHYT_RS01430at 325.246 kb on - strand, within BPHYT_RS01430at 325.246 kb on - strand, within BPHYT_RS01430at 325.246 kb on - strand, within BPHYT_RS01430at 325.246 kb on - strand, within BPHYT_RS01430at 325.246 kb on - strand, within BPHYT_RS01430at 325.246 kb on - strand, within BPHYT_RS01430at 325.320 kb on + strand, within BPHYT_RS01430at 325.320 kb on + strand, within BPHYT_RS01430at 325.321 kb on - strand, within BPHYT_RS01430at 325.376 kb on - strand, within BPHYT_RS01430at 325.376 kb on - strand, within BPHYT_RS01430at 325.476 kb on + strand, within BPHYT_RS01430at 325.476 kb on + strand, within BPHYT_RS01430at 325.477 kb on - strand, within BPHYT_RS01430at 325.477 kb on - strand, within BPHYT_RS01430at 325.477 kb on - strand, within BPHYT_RS01430at 325.477 kb on - strand, within BPHYT_RS01430at 325.477 kb on - strand, within BPHYT_RS01430

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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322,499 - -0.6
322,499 - +1.3
322,593 - BPHYT_RS01420 0.13 -0.9
322,626 + BPHYT_RS01420 0.16 +0.3
322,626 + BPHYT_RS01420 0.16 -1.5
322,627 - BPHYT_RS01420 0.16 +0.5
322,627 - BPHYT_RS01420 0.16 -0.1
322,656 + BPHYT_RS01420 0.20 +0.1
322,656 + BPHYT_RS01420 0.20 +0.0
322,659 + BPHYT_RS01420 0.20 -0.4
322,659 + BPHYT_RS01420 0.20 +0.5
322,659 + BPHYT_RS01420 0.20 -0.0
322,659 + BPHYT_RS01420 0.20 +0.1
322,659 + BPHYT_RS01420 0.20 +0.4
322,660 - BPHYT_RS01420 0.20 +0.3
322,737 + BPHYT_RS01420 0.29 +0.7
322,746 + BPHYT_RS01420 0.30 +0.5
322,771 - BPHYT_RS01420 0.33 -0.1
322,882 - BPHYT_RS01420 0.46 -0.5
322,882 - BPHYT_RS01420 0.46 -0.1
322,896 + BPHYT_RS01420 0.47 +0.4
322,896 + BPHYT_RS01420 0.47 -0.1
323,152 - BPHYT_RS01420 0.76 -0.8
323,202 + BPHYT_RS01420 0.82 +0.1
323,202 + BPHYT_RS01420 0.82 -0.1
323,202 + BPHYT_RS01420 0.82 -0.3
323,274 + +1.2
323,274 + -0.7
323,274 + +0.1
323,275 - +0.0
323,443 - +1.3
323,451 - +0.1
323,565 + -1.0
323,641 - BPHYT_RS01425 0.17 -0.2
323,846 + BPHYT_RS01425 0.37 -0.7
323,846 + BPHYT_RS01425 0.37 -2.1
324,030 - BPHYT_RS01425 0.55 -2.6
324,100 + BPHYT_RS01425 0.62 -1.1
324,175 - BPHYT_RS01425 0.70 -2.5
324,206 - BPHYT_RS01425 0.73 -0.4
324,484 + -0.7
324,484 + -1.7
324,484 + -3.0
324,485 - +0.8
324,485 - -0.7
324,485 - +0.7
324,527 - -0.1
324,553 - +0.6
324,588 - -0.1
324,591 - +0.1
324,591 - -0.9
324,595 - +0.4
324,643 - +0.5
324,676 - -0.0
324,831 - +0.8
325,002 + +1.4
325,078 - BPHYT_RS01430 0.12 -2.9
325,169 + BPHYT_RS01430 0.17 -0.1
325,169 + BPHYT_RS01430 0.17 -0.5
325,169 + BPHYT_RS01430 0.17 -2.0
325,170 - BPHYT_RS01430 0.17 +0.4
325,245 + BPHYT_RS01430 0.21 +0.2
325,245 + BPHYT_RS01430 0.21 +0.1
325,245 + BPHYT_RS01430 0.21 +0.6
325,245 + BPHYT_RS01430 0.21 +1.3
325,245 + BPHYT_RS01430 0.21 -0.7
325,245 + BPHYT_RS01430 0.21 -0.6
325,245 + BPHYT_RS01430 0.21 +0.2
325,245 + BPHYT_RS01430 0.21 +0.6
325,246 - BPHYT_RS01430 0.21 -0.2
325,246 - BPHYT_RS01430 0.21 -0.3
325,246 - BPHYT_RS01430 0.21 +0.4
325,246 - BPHYT_RS01430 0.21 -0.0
325,246 - BPHYT_RS01430 0.21 -0.4
325,246 - BPHYT_RS01430 0.21 +0.1
325,246 - BPHYT_RS01430 0.21 +0.3
325,246 - BPHYT_RS01430 0.21 -0.3
325,246 - BPHYT_RS01430 0.21 -0.1
325,246 - BPHYT_RS01430 0.21 +0.2
325,246 - BPHYT_RS01430 0.21 -0.1
325,246 - BPHYT_RS01430 0.21 -0.1
325,320 + BPHYT_RS01430 0.25 -0.7
325,320 + BPHYT_RS01430 0.25 -1.7
325,321 - BPHYT_RS01430 0.25 +0.1
325,376 - BPHYT_RS01430 0.28 -0.1
325,376 - BPHYT_RS01430 0.28 -0.1
325,476 + BPHYT_RS01430 0.33 -0.5
325,476 + BPHYT_RS01430 0.33 -0.6
325,477 - BPHYT_RS01430 0.33 -0.5
325,477 - BPHYT_RS01430 0.33 -0.2
325,477 - BPHYT_RS01430 0.33 +0.1
325,477 - BPHYT_RS01430 0.33 +0.7
325,477 - BPHYT_RS01430 0.33 +0.3

Or see this region's nucleotide sequence