Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01095 and BPHYT_RS01100 are separated by 9 nucleotides BPHYT_RS01100 and BPHYT_RS01105 overlap by 8 nucleotides BPHYT_RS01105 and BPHYT_RS01110 are separated by 114 nucleotides BPHYT_RS01110 and BPHYT_RS01115 are separated by 195 nucleotides BPHYT_RS01115 and BPHYT_RS01120 are separated by 139 nucleotides
BPHYT_RS01095: BPHYT_RS01095 - hypothetical protein, at 254,041 to 254,490
_RS01095
BPHYT_RS01100: BPHYT_RS01100 - protein mobD, at 254,500 to 255,204
_RS01100
BPHYT_RS01105: BPHYT_RS01105 - hypothetical protein, at 255,197 to 255,916
_RS01105
BPHYT_RS01110: BPHYT_RS01110 - hypothetical protein, at 256,031 to 256,237
_RS01110
BPHYT_RS01115: BPHYT_RS01115 - hypothetical protein, at 256,433 to 256,678
_RS01115
BPHYT_RS01120: BPHYT_RS01120 - plasmid partitioning protein ParB, at 256,818 to 258,902
_RS01120
Position (kb) 255
256 Strain fitness (log2 ratio) -2
-1
0
1 at 254.197 kb on + strand, within BPHYT_RS01095 at 254.536 kb on + strand at 254.536 kb on + strand at 254.537 kb on - strand at 254.537 kb on - strand at 254.537 kb on - strand at 254.623 kb on - strand, within BPHYT_RS01100 at 254.672 kb on + strand at 254.673 kb on - strand, within BPHYT_RS01100 at 254.740 kb on + strand, within BPHYT_RS01100 at 254.962 kb on + strand, within BPHYT_RS01100 at 254.963 kb on - strand, within BPHYT_RS01100 at 254.965 kb on + strand, within BPHYT_RS01100 at 254.966 kb on - strand, within BPHYT_RS01100 at 254.966 kb on - strand, within BPHYT_RS01100 at 254.993 kb on - strand, within BPHYT_RS01100 at 255.102 kb on - strand, within BPHYT_RS01100 at 255.166 kb on + strand at 255.167 kb on - strand at 255.167 kb on - strand at 255.167 kb on - strand at 255.371 kb on + strand, within BPHYT_RS01105 at 255.374 kb on + strand, within BPHYT_RS01105 at 255.386 kb on + strand, within BPHYT_RS01105 at 255.387 kb on - strand, within BPHYT_RS01105 at 256.566 kb on + strand, within BPHYT_RS01115 at 256.584 kb on + strand, within BPHYT_RS01115 at 256.617 kb on + strand, within BPHYT_RS01115 at 256.681 kb on + strand at 256.681 kb on + strand at 256.681 kb on + strand at 256.712 kb on - strand at 256.809 kb on + strand at 256.810 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 254,197 + BPHYT_RS01095 0.35 -0.7 254,536 + +1.0 254,536 + +0.1 254,537 - -0.7 254,537 - -0.2 254,537 - +1.2 254,623 - BPHYT_RS01100 0.17 -0.2 254,672 + -1.9 254,673 - BPHYT_RS01100 0.25 -0.8 254,740 + BPHYT_RS01100 0.34 +0.0 254,962 + BPHYT_RS01100 0.66 -0.0 254,963 - BPHYT_RS01100 0.66 +0.3 254,965 + BPHYT_RS01100 0.66 +0.1 254,966 - BPHYT_RS01100 0.66 +0.1 254,966 - BPHYT_RS01100 0.66 -0.5 254,993 - BPHYT_RS01100 0.70 -0.3 255,102 - BPHYT_RS01100 0.85 -0.1 255,166 + +0.8 255,167 - +0.5 255,167 - +0.6 255,167 - -0.8 255,371 + BPHYT_RS01105 0.24 +0.1 255,374 + BPHYT_RS01105 0.25 -1.5 255,386 + BPHYT_RS01105 0.26 -0.0 255,387 - BPHYT_RS01105 0.26 -1.1 256,566 + BPHYT_RS01115 0.54 -0.0 256,584 + BPHYT_RS01115 0.61 -1.1 256,617 + BPHYT_RS01115 0.75 +1.7 256,681 + -0.8 256,681 + +0.0 256,681 + -0.0 256,712 - -0.3 256,809 + +0.2 256,810 - -0.0
Or see this region's nucleotide sequence