Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS27835

Experiment: L-Threonine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS27825 and RR42_RS27830 overlap by 4 nucleotidesRR42_RS27830 and RR42_RS27835 are separated by 59 nucleotidesRR42_RS27835 and RR42_RS27840 are separated by 37 nucleotidesRR42_RS27840 and RR42_RS27845 are separated by 65 nucleotides RR42_RS27825: RR42_RS27825 - 4-hydroxyphenylacetate isomerase, at 1,607,156 to 1,607,887 _RS27825 RR42_RS27830: RR42_RS27830 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 1,607,884 to 1,608,648 _RS27830 RR42_RS27835: RR42_RS27835 - betaine-aldehyde dehydrogenase, at 1,608,708 to 1,610,168 _RS27835 RR42_RS27840: RR42_RS27840 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 1,610,206 to 1,611,054 _RS27840 RR42_RS27845: RR42_RS27845 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 1,611,120 to 1,611,509 _RS27845 Position (kb) 1608 1609 1610 1611Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1607.838 kb on + strandat 1607.838 kb on + strandat 1607.948 kb on + strandat 1607.948 kb on + strandat 1608.043 kb on + strand, within RR42_RS27830at 1608.043 kb on + strand, within RR42_RS27830at 1608.044 kb on - strand, within RR42_RS27830at 1608.138 kb on + strand, within RR42_RS27830at 1608.138 kb on + strand, within RR42_RS27830at 1608.139 kb on - strand, within RR42_RS27830at 1608.139 kb on - strand, within RR42_RS27830at 1608.139 kb on - strand, within RR42_RS27830at 1608.139 kb on - strand, within RR42_RS27830at 1608.139 kb on - strand, within RR42_RS27830at 1608.163 kb on + strand, within RR42_RS27830at 1608.164 kb on - strand, within RR42_RS27830at 1608.172 kb on + strand, within RR42_RS27830at 1608.173 kb on - strand, within RR42_RS27830at 1608.235 kb on + strand, within RR42_RS27830at 1608.235 kb on + strand, within RR42_RS27830at 1608.241 kb on + strand, within RR42_RS27830at 1608.241 kb on + strand, within RR42_RS27830at 1608.242 kb on - strand, within RR42_RS27830at 1608.272 kb on - strand, within RR42_RS27830at 1608.298 kb on + strand, within RR42_RS27830at 1608.298 kb on + strand, within RR42_RS27830at 1608.481 kb on + strand, within RR42_RS27830at 1608.482 kb on - strand, within RR42_RS27830at 1608.872 kb on + strand, within RR42_RS27835at 1608.872 kb on + strand, within RR42_RS27835at 1609.095 kb on + strand, within RR42_RS27835at 1609.096 kb on - strand, within RR42_RS27835at 1609.096 kb on - strand, within RR42_RS27835at 1609.695 kb on + strand, within RR42_RS27835at 1609.695 kb on + strand, within RR42_RS27835at 1609.696 kb on - strand, within RR42_RS27835at 1609.696 kb on - strand, within RR42_RS27835at 1609.923 kb on + strand, within RR42_RS27835at 1609.924 kb on - strand, within RR42_RS27835at 1609.924 kb on - strand, within RR42_RS27835at 1609.924 kb on - strand, within RR42_RS27835at 1609.938 kb on + strand, within RR42_RS27835at 1609.939 kb on - strand, within RR42_RS27835at 1610.147 kb on + strandat 1610.148 kb on - strandat 1610.216 kb on + strandat 1610.216 kb on + strandat 1610.431 kb on + strand, within RR42_RS27840at 1610.431 kb on + strand, within RR42_RS27840at 1610.431 kb on + strand, within RR42_RS27840at 1610.431 kb on + strand, within RR42_RS27840at 1610.474 kb on - strand, within RR42_RS27840at 1610.603 kb on - strand, within RR42_RS27840at 1610.897 kb on - strand, within RR42_RS27840at 1610.972 kb on - strandat 1610.973 kb on - strandat 1611.142 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Threonine (N)
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1,607,838 + +0.4
1,607,838 + -0.2
1,607,948 + -0.0
1,607,948 + +0.7
1,608,043 + RR42_RS27830 0.21 -0.1
1,608,043 + RR42_RS27830 0.21 -1.3
1,608,044 - RR42_RS27830 0.21 -1.6
1,608,138 + RR42_RS27830 0.33 +0.1
1,608,138 + RR42_RS27830 0.33 -0.5
1,608,139 - RR42_RS27830 0.33 +0.3
1,608,139 - RR42_RS27830 0.33 +0.5
1,608,139 - RR42_RS27830 0.33 -1.9
1,608,139 - RR42_RS27830 0.33 +0.1
1,608,139 - RR42_RS27830 0.33 +1.7
1,608,163 + RR42_RS27830 0.36 +0.4
1,608,164 - RR42_RS27830 0.37 +0.7
1,608,172 + RR42_RS27830 0.38 -1.3
1,608,173 - RR42_RS27830 0.38 +0.8
1,608,235 + RR42_RS27830 0.46 -1.1
1,608,235 + RR42_RS27830 0.46 +0.0
1,608,241 + RR42_RS27830 0.47 -0.4
1,608,241 + RR42_RS27830 0.47 -1.0
1,608,242 - RR42_RS27830 0.47 +1.1
1,608,272 - RR42_RS27830 0.51 +0.4
1,608,298 + RR42_RS27830 0.54 +2.0
1,608,298 + RR42_RS27830 0.54 +0.4
1,608,481 + RR42_RS27830 0.78 +0.3
1,608,482 - RR42_RS27830 0.78 +0.4
1,608,872 + RR42_RS27835 0.11 -0.4
1,608,872 + RR42_RS27835 0.11 +0.8
1,609,095 + RR42_RS27835 0.26 +1.1
1,609,096 - RR42_RS27835 0.27 -0.1
1,609,096 - RR42_RS27835 0.27 +1.5
1,609,695 + RR42_RS27835 0.68 -0.0
1,609,695 + RR42_RS27835 0.68 +0.0
1,609,696 - RR42_RS27835 0.68 +0.8
1,609,696 - RR42_RS27835 0.68 +1.2
1,609,923 + RR42_RS27835 0.83 -0.3
1,609,924 - RR42_RS27835 0.83 +0.5
1,609,924 - RR42_RS27835 0.83 -0.6
1,609,924 - RR42_RS27835 0.83 -1.2
1,609,938 + RR42_RS27835 0.84 -0.3
1,609,939 - RR42_RS27835 0.84 -3.1
1,610,147 + +0.4
1,610,148 - -0.8
1,610,216 + -0.2
1,610,216 + +1.9
1,610,431 + RR42_RS27840 0.27 -1.0
1,610,431 + RR42_RS27840 0.27 -0.3
1,610,431 + RR42_RS27840 0.27 -0.5
1,610,431 + RR42_RS27840 0.27 -0.2
1,610,474 - RR42_RS27840 0.32 -1.6
1,610,603 - RR42_RS27840 0.47 -0.1
1,610,897 - RR42_RS27840 0.81 -0.5
1,610,972 - -0.1
1,610,973 - -0.2
1,611,142 - +1.6

Or see this region's nucleotide sequence