Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS23955 and CA265_RS23960 are separated by 255 nucleotides CA265_RS23960 and CA265_RS23965 are separated by 82 nucleotides CA265_RS23965 and CA265_RS23970 overlap by 4 nucleotides
CA265_RS23955: CA265_RS23955 - glycosyl transferase family 4, at 5,840,593 to 5,841,795
_RS23955
CA265_RS23960: CA265_RS23960 - glycine cleavage system protein T, at 5,842,051 to 5,843,130
_RS23960
CA265_RS23965: CA265_RS23965 - 2-phosphosulfolactate phosphatase, at 5,843,213 to 5,843,929
_RS23965
CA265_RS23970: CA265_RS23970 - hypothetical protein, at 5,843,926 to 5,844,843
_RS23970
Position (kb) 5842
5843
5844 Strain fitness (log2 ratio) -1
0
1
2 at 5841.103 kb on - strand, within CA265_RS23955 at 5841.174 kb on + strand, within CA265_RS23955 at 5841.175 kb on - strand, within CA265_RS23955 at 5841.177 kb on - strand, within CA265_RS23955 at 5841.245 kb on + strand, within CA265_RS23955 at 5841.245 kb on + strand, within CA265_RS23955 at 5841.245 kb on + strand, within CA265_RS23955 at 5841.246 kb on - strand, within CA265_RS23955 at 5841.340 kb on - strand, within CA265_RS23955 at 5841.352 kb on - strand, within CA265_RS23955 at 5841.367 kb on - strand, within CA265_RS23955 at 5841.383 kb on - strand, within CA265_RS23955 at 5841.538 kb on - strand, within CA265_RS23955 at 5841.579 kb on + strand, within CA265_RS23955 at 5841.579 kb on + strand, within CA265_RS23955 at 5841.681 kb on + strand at 5841.798 kb on - strand at 5841.801 kb on + strand at 5841.801 kb on + strand at 5841.896 kb on - strand at 5841.996 kb on - strand at 5841.996 kb on - strand at 5841.996 kb on - strand at 5841.996 kb on - strand at 5842.006 kb on - strand at 5842.009 kb on - strand at 5842.193 kb on + strand, within CA265_RS23960 at 5842.555 kb on - strand, within CA265_RS23960 at 5842.860 kb on - strand, within CA265_RS23960 at 5843.170 kb on - strand at 5843.177 kb on + strand at 5843.262 kb on - strand at 5843.268 kb on + strand at 5843.927 kb on + strand at 5843.941 kb on + strand at 5843.941 kb on + strand at 5843.948 kb on - strand at 5843.950 kb on + strand at 5843.950 kb on + strand at 5843.951 kb on - strand at 5843.951 kb on - strand at 5843.982 kb on + strand at 5844.018 kb on - strand, within CA265_RS23970 at 5844.028 kb on + strand, within CA265_RS23970 at 5844.039 kb on - strand, within CA265_RS23970 at 5844.039 kb on - strand, within CA265_RS23970 at 5844.039 kb on - strand, within CA265_RS23970 at 5844.063 kb on - strand, within CA265_RS23970 at 5844.063 kb on - strand, within CA265_RS23970 at 5844.067 kb on - strand, within CA265_RS23970
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 5,841,103 - CA265_RS23955 0.42 +0.6 5,841,174 + CA265_RS23955 0.48 -0.3 5,841,175 - CA265_RS23955 0.48 +0.7 5,841,177 - CA265_RS23955 0.49 +0.3 5,841,245 + CA265_RS23955 0.54 -0.1 5,841,245 + CA265_RS23955 0.54 +1.1 5,841,245 + CA265_RS23955 0.54 -1.0 5,841,246 - CA265_RS23955 0.54 -0.6 5,841,340 - CA265_RS23955 0.62 -0.1 5,841,352 - CA265_RS23955 0.63 -0.4 5,841,367 - CA265_RS23955 0.64 +0.2 5,841,383 - CA265_RS23955 0.66 +0.5 5,841,538 - CA265_RS23955 0.79 +1.3 5,841,579 + CA265_RS23955 0.82 -0.2 5,841,579 + CA265_RS23955 0.82 +0.4 5,841,681 + +1.8 5,841,798 - +0.5 5,841,801 + -0.5 5,841,801 + -0.2 5,841,896 - +0.5 5,841,996 - +0.4 5,841,996 - +0.5 5,841,996 - +0.8 5,841,996 - -0.9 5,842,006 - +0.1 5,842,009 - +0.2 5,842,193 + CA265_RS23960 0.13 +0.8 5,842,555 - CA265_RS23960 0.47 -0.5 5,842,860 - CA265_RS23960 0.75 -1.5 5,843,170 - -0.2 5,843,177 + +0.7 5,843,262 - -0.2 5,843,268 + -0.2 5,843,927 + -0.5 5,843,941 + -0.4 5,843,941 + -0.5 5,843,948 - -0.6 5,843,950 + +0.0 5,843,950 + -0.3 5,843,951 - -0.0 5,843,951 - +0.6 5,843,982 + -0.9 5,844,018 - CA265_RS23970 0.10 -0.4 5,844,028 + CA265_RS23970 0.11 -0.3 5,844,039 - CA265_RS23970 0.12 +0.1 5,844,039 - CA265_RS23970 0.12 +0.1 5,844,039 - CA265_RS23970 0.12 +1.4 5,844,063 - CA265_RS23970 0.15 -0.1 5,844,063 - CA265_RS23970 0.15 +0.6 5,844,067 - CA265_RS23970 0.15 -0.4
Or see this region's nucleotide sequence