Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS23470 and CA265_RS23475 are separated by 15 nucleotides CA265_RS23475 and CA265_RS23480 are separated by 646 nucleotides CA265_RS23480 and CA265_RS23485 are separated by 85 nucleotides
CA265_RS23470: CA265_RS23470 - homoserine kinase (EC 2.7.1.39) (from data) , at 5,740,855 to 5,741,787
_RS23470
CA265_RS23475: CA265_RS23475 - aspartate kinase; homoserine dehydrogenase (EC 2.7.2.4; EC 1.1.1.3) (from data) , at 5,741,803 to 5,744,256
_RS23475
CA265_RS23480: CA265_RS23480 - hypothetical protein, at 5,744,903 to 5,745,097
_RS23480
CA265_RS23485: CA265_RS23485 - hypothetical protein, at 5,745,183 to 5,745,416
_RS23485
Position (kb)
5741
5742
5743
5744
5745 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 5740.831 kb on - strand at 5740.839 kb on + strand at 5740.853 kb on - strand at 5740.857 kb on - strand at 5741.092 kb on + strand, within CA265_RS23470 at 5741.139 kb on + strand, within CA265_RS23470 at 5741.140 kb on - strand, within CA265_RS23470 at 5741.239 kb on + strand, within CA265_RS23470 at 5741.270 kb on + strand, within CA265_RS23470 at 5741.290 kb on + strand, within CA265_RS23470 at 5741.299 kb on + strand, within CA265_RS23470 at 5741.300 kb on - strand, within CA265_RS23470 at 5741.316 kb on - strand, within CA265_RS23470 at 5741.341 kb on + strand, within CA265_RS23470 at 5741.435 kb on - strand, within CA265_RS23470 at 5741.435 kb on - strand, within CA265_RS23470 at 5741.546 kb on - strand, within CA265_RS23470 at 5741.546 kb on - strand, within CA265_RS23470 at 5741.546 kb on - strand, within CA265_RS23470 at 5741.552 kb on - strand, within CA265_RS23470 at 5741.569 kb on + strand, within CA265_RS23470 at 5741.569 kb on + strand, within CA265_RS23470 at 5741.582 kb on - strand, within CA265_RS23470 at 5741.594 kb on - strand, within CA265_RS23470 at 5741.722 kb on + strand at 5741.722 kb on + strand at 5741.741 kb on - strand at 5741.741 kb on - strand at 5741.778 kb on + strand at 5741.817 kb on + strand at 5741.821 kb on + strand at 5741.832 kb on + strand at 5741.905 kb on - strand at 5741.928 kb on + strand at 5741.929 kb on - strand at 5741.932 kb on - strand at 5741.932 kb on - strand at 5741.948 kb on - strand at 5741.953 kb on - strand at 5741.996 kb on - strand at 5742.001 kb on - strand at 5742.085 kb on - strand, within CA265_RS23475 at 5742.214 kb on - strand, within CA265_RS23475 at 5742.220 kb on - strand, within CA265_RS23475 at 5742.261 kb on + strand, within CA265_RS23475 at 5742.262 kb on - strand, within CA265_RS23475 at 5742.370 kb on + strand, within CA265_RS23475 at 5742.376 kb on - strand, within CA265_RS23475 at 5742.430 kb on + strand, within CA265_RS23475 at 5742.431 kb on - strand, within CA265_RS23475 at 5742.443 kb on + strand, within CA265_RS23475 at 5742.444 kb on - strand, within CA265_RS23475 at 5742.489 kb on + strand, within CA265_RS23475 at 5742.678 kb on + strand, within CA265_RS23475 at 5742.741 kb on + strand, within CA265_RS23475 at 5742.809 kb on - strand, within CA265_RS23475 at 5742.908 kb on - strand, within CA265_RS23475 at 5742.951 kb on + strand, within CA265_RS23475 at 5742.954 kb on - strand, within CA265_RS23475 at 5742.982 kb on + strand, within CA265_RS23475 at 5742.983 kb on - strand, within CA265_RS23475 at 5743.038 kb on + strand, within CA265_RS23475 at 5743.216 kb on - strand, within CA265_RS23475 at 5743.329 kb on + strand, within CA265_RS23475 at 5743.367 kb on - strand, within CA265_RS23475 at 5743.383 kb on - strand, within CA265_RS23475 at 5743.466 kb on + strand, within CA265_RS23475 at 5743.509 kb on + strand, within CA265_RS23475 at 5743.509 kb on + strand, within CA265_RS23475 at 5743.511 kb on + strand, within CA265_RS23475 at 5743.577 kb on - strand, within CA265_RS23475 at 5743.588 kb on + strand, within CA265_RS23475 at 5743.589 kb on - strand, within CA265_RS23475 at 5743.635 kb on + strand, within CA265_RS23475 at 5743.636 kb on - strand, within CA265_RS23475 at 5743.637 kb on + strand, within CA265_RS23475 at 5743.658 kb on - strand, within CA265_RS23475 at 5743.702 kb on - strand, within CA265_RS23475 at 5743.712 kb on - strand, within CA265_RS23475 at 5743.826 kb on + strand, within CA265_RS23475 at 5743.827 kb on - strand, within CA265_RS23475 at 5743.857 kb on + strand, within CA265_RS23475 at 5743.870 kb on - strand, within CA265_RS23475 at 5743.870 kb on - strand, within CA265_RS23475 at 5743.875 kb on + strand, within CA265_RS23475 at 5743.952 kb on - strand, within CA265_RS23475 at 5743.952 kb on - strand, within CA265_RS23475 at 5744.078 kb on + strand at 5744.119 kb on + strand at 5744.149 kb on + strand at 5744.155 kb on + strand at 5744.205 kb on + strand at 5744.224 kb on + strand at 5744.249 kb on - strand at 5744.262 kb on + strand at 5744.296 kb on + strand at 5744.296 kb on + strand at 5744.405 kb on + strand at 5744.447 kb on - strand at 5744.458 kb on + strand at 5744.493 kb on - strand at 5744.592 kb on - strand at 5744.659 kb on - strand at 5744.727 kb on + strand at 5744.815 kb on + strand at 5744.921 kb on + strand at 5744.944 kb on + strand, within CA265_RS23480 at 5744.992 kb on + strand, within CA265_RS23480 at 5744.992 kb on + strand, within CA265_RS23480 at 5744.993 kb on - strand, within CA265_RS23480 at 5745.001 kb on + strand, within CA265_RS23480 at 5745.038 kb on - strand, within CA265_RS23480 at 5745.039 kb on + strand, within CA265_RS23480 at 5745.040 kb on - strand, within CA265_RS23480 at 5745.120 kb on - strand at 5745.120 kb on - strand at 5745.120 kb on - strand at 5745.122 kb on - strand at 5745.122 kb on - strand at 5745.143 kb on + strand at 5745.181 kb on + strand at 5745.184 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 5,740,831 - +2.4 5,740,839 + -2.0 5,740,853 - -0.5 5,740,857 - +0.8 5,741,092 + CA265_RS23470 0.25 -3.7 5,741,139 + CA265_RS23470 0.30 -2.8 5,741,140 - CA265_RS23470 0.31 -3.5 5,741,239 + CA265_RS23470 0.41 -2.8 5,741,270 + CA265_RS23470 0.44 -1.3 5,741,290 + CA265_RS23470 0.47 -1.0 5,741,299 + CA265_RS23470 0.48 -2.8 5,741,300 - CA265_RS23470 0.48 -1.7 5,741,316 - CA265_RS23470 0.49 -2.2 5,741,341 + CA265_RS23470 0.52 -3.0 5,741,435 - CA265_RS23470 0.62 -2.5 5,741,435 - CA265_RS23470 0.62 -1.8 5,741,546 - CA265_RS23470 0.74 -0.2 5,741,546 - CA265_RS23470 0.74 -3.2 5,741,546 - CA265_RS23470 0.74 -2.8 5,741,552 - CA265_RS23470 0.75 -4.4 5,741,569 + CA265_RS23470 0.77 -3.6 5,741,569 + CA265_RS23470 0.77 -1.5 5,741,582 - CA265_RS23470 0.78 -1.8 5,741,594 - CA265_RS23470 0.79 -2.5 5,741,722 + -3.4 5,741,722 + -3.5 5,741,741 - -0.8 5,741,741 - -3.0 5,741,778 + -3.8 5,741,817 + -2.9 5,741,821 + -3.8 5,741,832 + -2.6 5,741,905 - -2.0 5,741,928 + -0.5 5,741,929 - -3.8 5,741,932 - -3.9 5,741,932 - -1.8 5,741,948 - -1.2 5,741,953 - -3.4 5,741,996 - -1.0 5,742,001 - -3.7 5,742,085 - CA265_RS23475 0.11 -1.3 5,742,214 - CA265_RS23475 0.17 -2.8 5,742,220 - CA265_RS23475 0.17 -2.1 5,742,261 + CA265_RS23475 0.19 -2.1 5,742,262 - CA265_RS23475 0.19 -1.9 5,742,370 + CA265_RS23475 0.23 -4.9 5,742,376 - CA265_RS23475 0.23 -4.2 5,742,430 + CA265_RS23475 0.26 -5.0 5,742,431 - CA265_RS23475 0.26 -1.8 5,742,443 + CA265_RS23475 0.26 -1.3 5,742,444 - CA265_RS23475 0.26 -2.9 5,742,489 + CA265_RS23475 0.28 -3.2 5,742,678 + CA265_RS23475 0.36 -2.1 5,742,741 + CA265_RS23475 0.38 -0.2 5,742,809 - CA265_RS23475 0.41 -0.1 5,742,908 - CA265_RS23475 0.45 -4.5 5,742,951 + CA265_RS23475 0.47 -4.5 5,742,954 - CA265_RS23475 0.47 -4.6 5,742,982 + CA265_RS23475 0.48 -3.8 5,742,983 - CA265_RS23475 0.48 -2.4 5,743,038 + CA265_RS23475 0.50 -4.3 5,743,216 - CA265_RS23475 0.58 -1.9 5,743,329 + CA265_RS23475 0.62 -4.8 5,743,367 - CA265_RS23475 0.64 -3.2 5,743,383 - CA265_RS23475 0.64 -2.3 5,743,466 + CA265_RS23475 0.68 -2.2 5,743,509 + CA265_RS23475 0.70 -4.6 5,743,509 + CA265_RS23475 0.70 -3.1 5,743,511 + CA265_RS23475 0.70 -3.9 5,743,577 - CA265_RS23475 0.72 -4.5 5,743,588 + CA265_RS23475 0.73 -4.2 5,743,589 - CA265_RS23475 0.73 -4.6 5,743,635 + CA265_RS23475 0.75 -2.2 5,743,636 - CA265_RS23475 0.75 -2.1 5,743,637 + CA265_RS23475 0.75 -2.6 5,743,658 - CA265_RS23475 0.76 -0.8 5,743,702 - CA265_RS23475 0.77 -2.0 5,743,712 - CA265_RS23475 0.78 -2.9 5,743,826 + CA265_RS23475 0.82 -2.1 5,743,827 - CA265_RS23475 0.82 -1.8 5,743,857 + CA265_RS23475 0.84 -2.6 5,743,870 - CA265_RS23475 0.84 -2.5 5,743,870 - CA265_RS23475 0.84 -0.8 5,743,875 + CA265_RS23475 0.84 -2.7 5,743,952 - CA265_RS23475 0.88 -3.1 5,743,952 - CA265_RS23475 0.88 -2.9 5,744,078 + -3.5 5,744,119 + -1.3 5,744,149 + -3.5 5,744,155 + -2.3 5,744,205 + -2.7 5,744,224 + -1.5 5,744,249 - -2.3 5,744,262 + -2.0 5,744,296 + -3.0 5,744,296 + -1.0 5,744,405 + -3.1 5,744,447 - -0.2 5,744,458 + -3.7 5,744,493 - -0.0 5,744,592 - +0.5 5,744,659 - +0.1 5,744,727 + +0.8 5,744,815 + -0.4 5,744,921 + -0.3 5,744,944 + CA265_RS23480 0.21 -0.6 5,744,992 + CA265_RS23480 0.46 -0.1 5,744,992 + CA265_RS23480 0.46 +0.1 5,744,993 - CA265_RS23480 0.46 +0.7 5,745,001 + CA265_RS23480 0.50 +0.6 5,745,038 - CA265_RS23480 0.69 -0.2 5,745,039 + CA265_RS23480 0.70 +0.3 5,745,040 - CA265_RS23480 0.70 -1.0 5,745,120 - +0.3 5,745,120 - -0.9 5,745,120 - +0.1 5,745,122 - -0.2 5,745,122 - +0.6 5,745,143 + -0.2 5,745,181 + -0.1 5,745,184 + -0.4
Or see this region's nucleotide sequence