Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22880 and CA265_RS22885 are separated by 112 nucleotides CA265_RS22885 and CA265_RS22890 are separated by 351 nucleotides CA265_RS22890 and CA265_RS22895 are separated by 50 nucleotides CA265_RS22895 and CA265_RS22900 are separated by 233 nucleotides
CA265_RS22880: CA265_RS22880 - amidohydrolase, at 5,603,308 to 5,606,328
_RS22880
CA265_RS22885: CA265_RS22885 - cytochrome B, at 5,606,441 to 5,606,884
_RS22885
CA265_RS22890: CA265_RS22890 - anthranilate synthase component I, at 5,607,236 to 5,608,636
_RS22890
CA265_RS22895: CA265_RS22895 - aminodeoxychorismate/anthranilate synthase component II, at 5,608,687 to 5,609,283
_RS22895
CA265_RS22900: CA265_RS22900 - indole-3-glycerol phosphate synthase, at 5,609,517 to 5,610,299
_RS22900
Position (kb)
5607
5608
5609 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5606.331 kb on + strand at 5606.406 kb on + strand at 5606.406 kb on + strand at 5606.406 kb on + strand at 5606.430 kb on - strand at 5606.490 kb on + strand, within CA265_RS22885 at 5606.504 kb on - strand, within CA265_RS22885 at 5606.512 kb on - strand, within CA265_RS22885 at 5606.581 kb on + strand, within CA265_RS22885 at 5606.668 kb on + strand, within CA265_RS22885 at 5606.696 kb on - strand, within CA265_RS22885 at 5606.728 kb on + strand, within CA265_RS22885 at 5606.729 kb on - strand, within CA265_RS22885 at 5606.846 kb on - strand at 5606.846 kb on - strand at 5606.883 kb on - strand at 5606.940 kb on - strand at 5606.942 kb on - strand at 5606.984 kb on - strand at 5607.021 kb on - strand at 5607.047 kb on + strand at 5607.047 kb on + strand at 5607.047 kb on + strand at 5607.048 kb on - strand at 5607.048 kb on - strand at 5607.051 kb on + strand at 5607.051 kb on + strand at 5607.051 kb on + strand at 5607.051 kb on + strand at 5607.052 kb on - strand at 5607.052 kb on - strand at 5607.070 kb on - strand at 5607.132 kb on + strand at 5607.206 kb on + strand at 5607.213 kb on + strand at 5607.303 kb on - strand at 5607.441 kb on - strand, within CA265_RS22890 at 5607.588 kb on + strand, within CA265_RS22890 at 5607.605 kb on - strand, within CA265_RS22890 at 5607.676 kb on + strand, within CA265_RS22890 at 5607.794 kb on + strand, within CA265_RS22890 at 5607.795 kb on - strand, within CA265_RS22890 at 5607.878 kb on - strand, within CA265_RS22890 at 5607.878 kb on - strand, within CA265_RS22890 at 5607.878 kb on - strand, within CA265_RS22890 at 5607.956 kb on + strand, within CA265_RS22890 at 5607.956 kb on + strand, within CA265_RS22890 at 5608.181 kb on - strand, within CA265_RS22890 at 5608.247 kb on - strand, within CA265_RS22890 at 5608.262 kb on + strand, within CA265_RS22890 at 5608.347 kb on - strand, within CA265_RS22890 at 5608.357 kb on + strand, within CA265_RS22890 at 5608.450 kb on + strand, within CA265_RS22890 at 5608.645 kb on + strand at 5608.678 kb on - strand at 5608.683 kb on + strand at 5608.695 kb on + strand at 5608.906 kb on + strand, within CA265_RS22895 at 5608.907 kb on - strand, within CA265_RS22895 at 5608.940 kb on + strand, within CA265_RS22895 at 5608.983 kb on - strand, within CA265_RS22895 at 5609.073 kb on - strand, within CA265_RS22895 at 5609.087 kb on - strand, within CA265_RS22895 at 5609.152 kb on - strand, within CA265_RS22895 at 5609.163 kb on + strand, within CA265_RS22895 at 5609.187 kb on - strand, within CA265_RS22895 at 5609.202 kb on - strand, within CA265_RS22895 at 5609.272 kb on - strand at 5609.363 kb on + strand at 5609.364 kb on - strand at 5609.368 kb on - strand at 5609.368 kb on - strand at 5609.370 kb on - strand at 5609.407 kb on - strand at 5609.629 kb on - strand, within CA265_RS22900
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 5,606,331 + -0.1 5,606,406 + -0.4 5,606,406 + -0.8 5,606,406 + -0.4 5,606,430 - +1.8 5,606,490 + CA265_RS22885 0.11 -0.7 5,606,504 - CA265_RS22885 0.14 -1.4 5,606,512 - CA265_RS22885 0.16 -0.5 5,606,581 + CA265_RS22885 0.32 +0.1 5,606,668 + CA265_RS22885 0.51 +0.1 5,606,696 - CA265_RS22885 0.57 -0.5 5,606,728 + CA265_RS22885 0.65 -0.0 5,606,729 - CA265_RS22885 0.65 -0.7 5,606,846 - -0.4 5,606,846 - +0.7 5,606,883 - -0.8 5,606,940 - +0.8 5,606,942 - -0.3 5,606,984 - +0.1 5,607,021 - -0.1 5,607,047 + -0.0 5,607,047 + +0.4 5,607,047 + -0.0 5,607,048 - -1.9 5,607,048 - +0.5 5,607,051 + +0.8 5,607,051 + +0.7 5,607,051 + +0.3 5,607,051 + +1.8 5,607,052 - -1.1 5,607,052 - -0.7 5,607,070 - -1.0 5,607,132 + -0.0 5,607,206 + -1.4 5,607,213 + -0.3 5,607,303 - -1.0 5,607,441 - CA265_RS22890 0.15 +1.8 5,607,588 + CA265_RS22890 0.25 -0.9 5,607,605 - CA265_RS22890 0.26 -2.4 5,607,676 + CA265_RS22890 0.31 -1.3 5,607,794 + CA265_RS22890 0.40 -1.3 5,607,795 - CA265_RS22890 0.40 -1.3 5,607,878 - CA265_RS22890 0.46 -0.5 5,607,878 - CA265_RS22890 0.46 -3.2 5,607,878 - CA265_RS22890 0.46 -1.4 5,607,956 + CA265_RS22890 0.51 -0.0 5,607,956 + CA265_RS22890 0.51 -1.2 5,608,181 - CA265_RS22890 0.67 -2.5 5,608,247 - CA265_RS22890 0.72 -1.9 5,608,262 + CA265_RS22890 0.73 -1.5 5,608,347 - CA265_RS22890 0.79 -1.6 5,608,357 + CA265_RS22890 0.80 -2.5 5,608,450 + CA265_RS22890 0.87 -1.0 5,608,645 + +0.0 5,608,678 - -2.5 5,608,683 + +0.6 5,608,695 + -0.2 5,608,906 + CA265_RS22895 0.37 -0.1 5,608,907 - CA265_RS22895 0.37 -0.3 5,608,940 + CA265_RS22895 0.42 +0.3 5,608,983 - CA265_RS22895 0.50 -1.7 5,609,073 - CA265_RS22895 0.65 -1.6 5,609,087 - CA265_RS22895 0.67 -1.9 5,609,152 - CA265_RS22895 0.78 -1.8 5,609,163 + CA265_RS22895 0.80 -0.2 5,609,187 - CA265_RS22895 0.84 -1.3 5,609,202 - CA265_RS22895 0.86 -1.2 5,609,272 - -0.8 5,609,363 + +0.1 5,609,364 - -1.2 5,609,368 - -3.1 5,609,368 - -2.9 5,609,370 - -0.7 5,609,407 - -1.2 5,609,629 - CA265_RS22900 0.14 +1.8
Or see this region's nucleotide sequence