Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22775 and CA265_RS22780 are separated by 15 nucleotides CA265_RS22780 and CA265_RS22785 are separated by 15 nucleotides CA265_RS22785 and CA265_RS22790 overlap by 8 nucleotides CA265_RS22790 and CA265_RS22795 are separated by 4 nucleotides
CA265_RS22775: CA265_RS22775 - glycosyl hydrolase, at 5,572,769 to 5,573,884
_RS22775
CA265_RS22780: CA265_RS22780 - hypothetical protein, at 5,573,900 to 5,574,799
_RS22780
CA265_RS22785: CA265_RS22785 - hypothetical protein, at 5,574,815 to 5,575,822
_RS22785
CA265_RS22790: CA265_RS22790 - hypothetical protein, at 5,575,815 to 5,576,648
_RS22790
CA265_RS22795: CA265_RS22795 - antibiotic ABC transporter ATP-binding protein, at 5,576,653 to 5,578,470
_RS22795
Position (kb) 5574
5575
5576 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5573.834 kb on + strand at 5573.839 kb on - strand at 5573.839 kb on - strand at 5573.901 kb on + strand at 5573.901 kb on + strand at 5573.902 kb on - strand at 5574.817 kb on - strand at 5574.885 kb on + strand at 5575.004 kb on - strand, within CA265_RS22785 at 5575.058 kb on - strand, within CA265_RS22785 at 5575.113 kb on + strand, within CA265_RS22785 at 5575.114 kb on - strand, within CA265_RS22785 at 5575.167 kb on - strand, within CA265_RS22785 at 5575.449 kb on + strand, within CA265_RS22785 at 5575.508 kb on + strand, within CA265_RS22785 at 5575.622 kb on + strand, within CA265_RS22785 at 5575.623 kb on - strand, within CA265_RS22785 at 5575.647 kb on + strand, within CA265_RS22785 at 5575.669 kb on + strand, within CA265_RS22785 at 5575.702 kb on + strand, within CA265_RS22785 at 5575.702 kb on + strand, within CA265_RS22785 at 5575.702 kb on + strand, within CA265_RS22785 at 5575.746 kb on - strand at 5575.790 kb on - strand at 5575.817 kb on - strand at 5575.830 kb on - strand at 5575.837 kb on - strand at 5575.837 kb on - strand at 5575.840 kb on - strand at 5575.870 kb on + strand at 5575.880 kb on + strand at 5575.880 kb on + strand at 5575.943 kb on - strand, within CA265_RS22790 at 5575.943 kb on - strand, within CA265_RS22790 at 5575.994 kb on - strand, within CA265_RS22790 at 5576.016 kb on + strand, within CA265_RS22790 at 5576.061 kb on - strand, within CA265_RS22790 at 5576.064 kb on - strand, within CA265_RS22790 at 5576.067 kb on - strand, within CA265_RS22790 at 5576.094 kb on + strand, within CA265_RS22790 at 5576.243 kb on + strand, within CA265_RS22790 at 5576.383 kb on - strand, within CA265_RS22790 at 5576.388 kb on - strand, within CA265_RS22790 at 5576.391 kb on - strand, within CA265_RS22790 at 5576.411 kb on + strand, within CA265_RS22790 at 5576.413 kb on + strand, within CA265_RS22790 at 5576.413 kb on + strand, within CA265_RS22790 at 5576.414 kb on - strand, within CA265_RS22790 at 5576.498 kb on - strand, within CA265_RS22790 at 5576.529 kb on - strand, within CA265_RS22790 at 5576.579 kb on + strand at 5576.580 kb on - strand at 5576.585 kb on + strand at 5576.585 kb on + strand at 5576.585 kb on + strand at 5576.596 kb on + strand at 5576.596 kb on + strand at 5576.597 kb on - strand at 5576.603 kb on - strand at 5576.643 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 5,573,834 + +0.2 5,573,839 - +0.8 5,573,839 - -2.0 5,573,901 + +0.0 5,573,901 + +0.6 5,573,902 - +0.6 5,574,817 - -1.0 5,574,885 + +0.0 5,575,004 - CA265_RS22785 0.19 -1.2 5,575,058 - CA265_RS22785 0.24 -1.7 5,575,113 + CA265_RS22785 0.30 -0.6 5,575,114 - CA265_RS22785 0.30 -2.0 5,575,167 - CA265_RS22785 0.35 +0.8 5,575,449 + CA265_RS22785 0.63 +0.8 5,575,508 + CA265_RS22785 0.69 -1.5 5,575,622 + CA265_RS22785 0.80 +0.1 5,575,623 - CA265_RS22785 0.80 -1.2 5,575,647 + CA265_RS22785 0.83 -1.0 5,575,669 + CA265_RS22785 0.85 -0.2 5,575,702 + CA265_RS22785 0.88 -3.0 5,575,702 + CA265_RS22785 0.88 -1.0 5,575,702 + CA265_RS22785 0.88 -1.1 5,575,746 - -1.2 5,575,790 - -0.0 5,575,817 - -0.6 5,575,830 - -0.7 5,575,837 - -0.1 5,575,837 - +0.8 5,575,840 - -0.9 5,575,870 + +0.1 5,575,880 + +0.4 5,575,880 + +0.3 5,575,943 - CA265_RS22790 0.15 -2.9 5,575,943 - CA265_RS22790 0.15 -1.0 5,575,994 - CA265_RS22790 0.21 +0.0 5,576,016 + CA265_RS22790 0.24 +0.7 5,576,061 - CA265_RS22790 0.29 -0.6 5,576,064 - CA265_RS22790 0.30 -0.6 5,576,067 - CA265_RS22790 0.30 +0.4 5,576,094 + CA265_RS22790 0.33 -0.0 5,576,243 + CA265_RS22790 0.51 -0.3 5,576,383 - CA265_RS22790 0.68 +0.9 5,576,388 - CA265_RS22790 0.69 +0.1 5,576,391 - CA265_RS22790 0.69 +0.8 5,576,411 + CA265_RS22790 0.71 -1.3 5,576,413 + CA265_RS22790 0.72 +0.9 5,576,413 + CA265_RS22790 0.72 -0.3 5,576,414 - CA265_RS22790 0.72 -0.7 5,576,498 - CA265_RS22790 0.82 -0.5 5,576,529 - CA265_RS22790 0.86 +0.3 5,576,579 + -0.1 5,576,580 - +0.3 5,576,585 + +0.5 5,576,585 + +0.0 5,576,585 + +0.3 5,576,596 + +1.3 5,576,596 + +0.8 5,576,597 - +0.6 5,576,603 - +0.5 5,576,643 - +0.0
Or see this region's nucleotide sequence