Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS14995 and CA265_RS15000 are separated by 115 nucleotides CA265_RS15000 and CA265_RS15005 are separated by 97 nucleotides
CA265_RS14995: CA265_RS14995 - sodium-translocating pyrophosphatase, at 3,778,646 to 3,780,892
_RS14995
CA265_RS15000: CA265_RS15000 - amino acid permease, at 3,781,008 to 3,782,483
_RS15000
CA265_RS15005: CA265_RS15005 - porin, at 3,782,581 to 3,783,912
_RS15005
Position (kb)
3781
3782
3783 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3780.064 kb on - strand, within CA265_RS14995 at 3780.148 kb on - strand, within CA265_RS14995 at 3780.162 kb on + strand, within CA265_RS14995 at 3780.162 kb on + strand, within CA265_RS14995 at 3780.241 kb on - strand, within CA265_RS14995 at 3780.247 kb on + strand, within CA265_RS14995 at 3780.248 kb on - strand, within CA265_RS14995 at 3780.352 kb on + strand, within CA265_RS14995 at 3780.360 kb on - strand, within CA265_RS14995 at 3780.361 kb on + strand, within CA265_RS14995 at 3780.361 kb on + strand, within CA265_RS14995 at 3780.362 kb on - strand, within CA265_RS14995 at 3780.378 kb on - strand, within CA265_RS14995 at 3780.451 kb on + strand, within CA265_RS14995 at 3780.501 kb on + strand, within CA265_RS14995 at 3780.502 kb on - strand, within CA265_RS14995 at 3780.502 kb on - strand, within CA265_RS14995 at 3780.528 kb on + strand, within CA265_RS14995 at 3780.529 kb on - strand, within CA265_RS14995 at 3780.724 kb on - strand at 3780.745 kb on + strand at 3780.755 kb on + strand at 3780.756 kb on - strand at 3780.756 kb on - strand at 3780.838 kb on - strand at 3780.844 kb on + strand at 3780.909 kb on - strand at 3780.909 kb on - strand at 3780.909 kb on - strand at 3780.987 kb on - strand at 3781.004 kb on + strand at 3781.007 kb on + strand at 3781.040 kb on - strand at 3781.142 kb on - strand at 3781.216 kb on + strand, within CA265_RS15000 at 3781.217 kb on - strand, within CA265_RS15000 at 3781.237 kb on + strand, within CA265_RS15000 at 3781.238 kb on - strand, within CA265_RS15000 at 3781.285 kb on - strand, within CA265_RS15000 at 3781.287 kb on - strand, within CA265_RS15000 at 3781.287 kb on - strand, within CA265_RS15000 at 3781.287 kb on - strand, within CA265_RS15000 at 3781.290 kb on + strand, within CA265_RS15000 at 3781.292 kb on + strand, within CA265_RS15000 at 3781.296 kb on + strand, within CA265_RS15000 at 3781.316 kb on - strand, within CA265_RS15000 at 3781.354 kb on - strand, within CA265_RS15000 at 3781.370 kb on + strand, within CA265_RS15000 at 3781.375 kb on + strand, within CA265_RS15000 at 3781.376 kb on - strand, within CA265_RS15000 at 3781.376 kb on - strand, within CA265_RS15000 at 3781.408 kb on + strand, within CA265_RS15000 at 3781.409 kb on - strand, within CA265_RS15000 at 3781.423 kb on + strand, within CA265_RS15000 at 3781.424 kb on - strand, within CA265_RS15000 at 3781.427 kb on + strand, within CA265_RS15000 at 3781.427 kb on + strand, within CA265_RS15000 at 3781.428 kb on - strand, within CA265_RS15000 at 3781.490 kb on - strand, within CA265_RS15000 at 3781.618 kb on + strand, within CA265_RS15000 at 3781.689 kb on + strand, within CA265_RS15000 at 3781.689 kb on + strand, within CA265_RS15000 at 3781.812 kb on - strand, within CA265_RS15000 at 3781.866 kb on - strand, within CA265_RS15000 at 3781.873 kb on + strand, within CA265_RS15000 at 3781.873 kb on + strand, within CA265_RS15000 at 3781.874 kb on - strand, within CA265_RS15000 at 3781.875 kb on + strand, within CA265_RS15000 at 3781.876 kb on - strand, within CA265_RS15000 at 3781.877 kb on + strand, within CA265_RS15000 at 3781.951 kb on + strand, within CA265_RS15000 at 3781.998 kb on - strand, within CA265_RS15000 at 3782.003 kb on - strand, within CA265_RS15000 at 3782.039 kb on - strand, within CA265_RS15000 at 3782.176 kb on + strand, within CA265_RS15000 at 3782.234 kb on + strand, within CA265_RS15000 at 3782.235 kb on - strand, within CA265_RS15000 at 3782.249 kb on + strand, within CA265_RS15000 at 3782.270 kb on - strand, within CA265_RS15000 at 3782.365 kb on + strand at 3782.451 kb on - strand at 3782.538 kb on + strand at 3782.542 kb on + strand at 3782.614 kb on + strand at 3782.615 kb on - strand at 3782.659 kb on + strand at 3782.748 kb on + strand, within CA265_RS15005 at 3782.759 kb on + strand, within CA265_RS15005 at 3782.759 kb on + strand, within CA265_RS15005 at 3782.806 kb on + strand, within CA265_RS15005 at 3782.853 kb on - strand, within CA265_RS15005 at 3782.867 kb on + strand, within CA265_RS15005 at 3782.878 kb on + strand, within CA265_RS15005 at 3782.878 kb on + strand, within CA265_RS15005 at 3782.878 kb on + strand, within CA265_RS15005 at 3782.878 kb on + strand, within CA265_RS15005 at 3782.879 kb on - strand, within CA265_RS15005 at 3782.879 kb on - strand, within CA265_RS15005 at 3782.880 kb on + strand, within CA265_RS15005 at 3782.910 kb on + strand, within CA265_RS15005 at 3782.914 kb on + strand, within CA265_RS15005 at 3782.977 kb on + strand, within CA265_RS15005 at 3782.978 kb on - strand, within CA265_RS15005 at 3782.981 kb on - strand, within CA265_RS15005 at 3782.988 kb on + strand, within CA265_RS15005 at 3782.988 kb on + strand, within CA265_RS15005 at 3783.132 kb on + strand, within CA265_RS15005 at 3783.133 kb on - strand, within CA265_RS15005 at 3783.139 kb on - strand, within CA265_RS15005 at 3783.199 kb on + strand, within CA265_RS15005 at 3783.244 kb on - strand, within CA265_RS15005 at 3783.299 kb on + strand, within CA265_RS15005 at 3783.300 kb on - strand, within CA265_RS15005 at 3783.315 kb on + strand, within CA265_RS15005 at 3783.321 kb on - strand, within CA265_RS15005 at 3783.356 kb on - strand, within CA265_RS15005 at 3783.440 kb on - strand, within CA265_RS15005 at 3783.440 kb on - strand, within CA265_RS15005 at 3783.442 kb on + strand, within CA265_RS15005 at 3783.443 kb on - strand, within CA265_RS15005 at 3783.466 kb on - strand, within CA265_RS15005
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,780,064 - CA265_RS14995 0.63 +0.2 3,780,148 - CA265_RS14995 0.67 -0.8 3,780,162 + CA265_RS14995 0.67 -1.0 3,780,162 + CA265_RS14995 0.67 -0.2 3,780,241 - CA265_RS14995 0.71 -0.5 3,780,247 + CA265_RS14995 0.71 -0.3 3,780,248 - CA265_RS14995 0.71 -0.6 3,780,352 + CA265_RS14995 0.76 -0.6 3,780,360 - CA265_RS14995 0.76 -0.2 3,780,361 + CA265_RS14995 0.76 +0.1 3,780,361 + CA265_RS14995 0.76 -0.0 3,780,362 - CA265_RS14995 0.76 -0.2 3,780,378 - CA265_RS14995 0.77 +0.7 3,780,451 + CA265_RS14995 0.80 -0.4 3,780,501 + CA265_RS14995 0.83 -0.0 3,780,502 - CA265_RS14995 0.83 -0.1 3,780,502 - CA265_RS14995 0.83 -2.2 3,780,528 + CA265_RS14995 0.84 -0.7 3,780,529 - CA265_RS14995 0.84 +1.1 3,780,724 - +0.7 3,780,745 + -1.3 3,780,755 + +0.0 3,780,756 - -2.4 3,780,756 - -0.8 3,780,838 - -0.4 3,780,844 + +1.2 3,780,909 - -0.1 3,780,909 - -0.0 3,780,909 - +0.2 3,780,987 - -1.7 3,781,004 + -2.1 3,781,007 + -1.3 3,781,040 - -2.1 3,781,142 - +0.0 3,781,216 + CA265_RS15000 0.14 +1.8 3,781,217 - CA265_RS15000 0.14 -0.5 3,781,237 + CA265_RS15000 0.16 -2.5 3,781,238 - CA265_RS15000 0.16 -0.8 3,781,285 - CA265_RS15000 0.19 -3.0 3,781,287 - CA265_RS15000 0.19 +1.5 3,781,287 - CA265_RS15000 0.19 -1.2 3,781,287 - CA265_RS15000 0.19 -3.4 3,781,290 + CA265_RS15000 0.19 -1.6 3,781,292 + CA265_RS15000 0.19 -1.5 3,781,296 + CA265_RS15000 0.20 -1.7 3,781,316 - CA265_RS15000 0.21 -4.1 3,781,354 - CA265_RS15000 0.23 +0.3 3,781,370 + CA265_RS15000 0.25 -1.4 3,781,375 + CA265_RS15000 0.25 -1.0 3,781,376 - CA265_RS15000 0.25 -1.2 3,781,376 - CA265_RS15000 0.25 -0.3 3,781,408 + CA265_RS15000 0.27 -2.7 3,781,409 - CA265_RS15000 0.27 -0.2 3,781,423 + CA265_RS15000 0.28 -1.5 3,781,424 - CA265_RS15000 0.28 -1.0 3,781,427 + CA265_RS15000 0.28 -0.6 3,781,427 + CA265_RS15000 0.28 -0.4 3,781,428 - CA265_RS15000 0.28 -1.2 3,781,490 - CA265_RS15000 0.33 -2.6 3,781,618 + CA265_RS15000 0.41 -3.4 3,781,689 + CA265_RS15000 0.46 -1.9 3,781,689 + CA265_RS15000 0.46 -1.2 3,781,812 - CA265_RS15000 0.54 -1.8 3,781,866 - CA265_RS15000 0.58 -2.1 3,781,873 + CA265_RS15000 0.59 -2.1 3,781,873 + CA265_RS15000 0.59 -1.9 3,781,874 - CA265_RS15000 0.59 -1.2 3,781,875 + CA265_RS15000 0.59 -1.0 3,781,876 - CA265_RS15000 0.59 -1.9 3,781,877 + CA265_RS15000 0.59 -2.4 3,781,951 + CA265_RS15000 0.64 +0.2 3,781,998 - CA265_RS15000 0.67 -1.2 3,782,003 - CA265_RS15000 0.67 -0.9 3,782,039 - CA265_RS15000 0.70 -1.5 3,782,176 + CA265_RS15000 0.79 -2.8 3,782,234 + CA265_RS15000 0.83 -2.9 3,782,235 - CA265_RS15000 0.83 -1.5 3,782,249 + CA265_RS15000 0.84 +1.4 3,782,270 - CA265_RS15000 0.86 -2.2 3,782,365 + -1.0 3,782,451 - -0.5 3,782,538 + -0.6 3,782,542 + -1.0 3,782,614 + -0.0 3,782,615 - -0.9 3,782,659 + +0.2 3,782,748 + CA265_RS15005 0.13 -1.3 3,782,759 + CA265_RS15005 0.13 +0.5 3,782,759 + CA265_RS15005 0.13 -0.3 3,782,806 + CA265_RS15005 0.17 -0.6 3,782,853 - CA265_RS15005 0.20 +0.6 3,782,867 + CA265_RS15005 0.21 +0.2 3,782,878 + CA265_RS15005 0.22 +0.3 3,782,878 + CA265_RS15005 0.22 -0.3 3,782,878 + CA265_RS15005 0.22 -0.3 3,782,878 + CA265_RS15005 0.22 +0.1 3,782,879 - CA265_RS15005 0.22 -0.5 3,782,879 - CA265_RS15005 0.22 -1.2 3,782,880 + CA265_RS15005 0.22 +1.3 3,782,910 + CA265_RS15005 0.25 -0.5 3,782,914 + CA265_RS15005 0.25 -1.8 3,782,977 + CA265_RS15005 0.30 +0.6 3,782,978 - CA265_RS15005 0.30 -0.3 3,782,981 - CA265_RS15005 0.30 -0.4 3,782,988 + CA265_RS15005 0.31 -1.1 3,782,988 + CA265_RS15005 0.31 +0.6 3,783,132 + CA265_RS15005 0.41 -0.4 3,783,133 - CA265_RS15005 0.41 -0.5 3,783,139 - CA265_RS15005 0.42 -0.7 3,783,199 + CA265_RS15005 0.46 -0.9 3,783,244 - CA265_RS15005 0.50 -0.3 3,783,299 + CA265_RS15005 0.54 -0.5 3,783,300 - CA265_RS15005 0.54 +0.1 3,783,315 + CA265_RS15005 0.55 -0.2 3,783,321 - CA265_RS15005 0.56 -0.5 3,783,356 - CA265_RS15005 0.58 -0.9 3,783,440 - CA265_RS15005 0.64 +0.4 3,783,440 - CA265_RS15005 0.64 -0.6 3,783,442 + CA265_RS15005 0.65 +0.2 3,783,443 - CA265_RS15005 0.65 -0.0 3,783,466 - CA265_RS15005 0.66 +0.2
Or see this region's nucleotide sequence