Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03730 and CA265_RS03735 overlap by 4 nucleotides CA265_RS03735 and CA265_RS03740 are separated by 42 nucleotides
CA265_RS03730: CA265_RS03730 - glycosyltransferase, at 886,519 to 887,487
_RS03730
CA265_RS03735: CA265_RS03735 - hypothetical protein, at 887,484 to 888,272
_RS03735
CA265_RS03740: CA265_RS03740 - hypothetical protein, at 888,315 to 889,862
_RS03740
Position (kb) 887
888
889 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 887.456 kb on + strand at 887.576 kb on + strand, within CA265_RS03735 at 887.620 kb on + strand, within CA265_RS03735 at 887.664 kb on - strand, within CA265_RS03735 at 887.735 kb on - strand, within CA265_RS03735 at 887.798 kb on + strand, within CA265_RS03735 at 887.813 kb on + strand, within CA265_RS03735 at 887.813 kb on + strand, within CA265_RS03735 at 887.813 kb on + strand, within CA265_RS03735 at 887.821 kb on - strand, within CA265_RS03735 at 887.909 kb on + strand, within CA265_RS03735 at 887.993 kb on + strand, within CA265_RS03735 at 888.038 kb on + strand, within CA265_RS03735 at 888.039 kb on - strand, within CA265_RS03735 at 888.117 kb on + strand, within CA265_RS03735 at 888.230 kb on + strand at 888.230 kb on + strand at 888.231 kb on - strand at 888.257 kb on - strand at 888.296 kb on - strand at 888.371 kb on + strand at 888.382 kb on + strand at 888.571 kb on - strand, within CA265_RS03740 at 888.605 kb on - strand, within CA265_RS03740 at 888.752 kb on + strand, within CA265_RS03740 at 888.863 kb on - strand, within CA265_RS03740 at 888.866 kb on - strand, within CA265_RS03740 at 888.866 kb on - strand, within CA265_RS03740 at 888.905 kb on - strand, within CA265_RS03740 at 889.024 kb on - strand, within CA265_RS03740 at 889.098 kb on + strand, within CA265_RS03740 at 889.203 kb on + strand, within CA265_RS03740
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 887,456 + -0.1 887,576 + CA265_RS03735 0.12 -1.2 887,620 + CA265_RS03735 0.17 +0.2 887,664 - CA265_RS03735 0.23 -1.2 887,735 - CA265_RS03735 0.32 -2.2 887,798 + CA265_RS03735 0.40 -2.8 887,813 + CA265_RS03735 0.42 -0.7 887,813 + CA265_RS03735 0.42 -2.2 887,813 + CA265_RS03735 0.42 -1.0 887,821 - CA265_RS03735 0.43 -1.2 887,909 + CA265_RS03735 0.54 -0.6 887,993 + CA265_RS03735 0.65 +0.2 888,038 + CA265_RS03735 0.70 -0.4 888,039 - CA265_RS03735 0.70 -2.8 888,117 + CA265_RS03735 0.80 -0.8 888,230 + -0.1 888,230 + -0.8 888,231 - -1.0 888,257 - -3.2 888,296 - -1.0 888,371 + -1.4 888,382 + -1.1 888,571 - CA265_RS03740 0.17 -2.1 888,605 - CA265_RS03740 0.19 -1.8 888,752 + CA265_RS03740 0.28 -1.1 888,863 - CA265_RS03740 0.35 -1.5 888,866 - CA265_RS03740 0.36 -1.6 888,866 - CA265_RS03740 0.36 -2.6 888,905 - CA265_RS03740 0.38 -1.4 889,024 - CA265_RS03740 0.46 -1.6 889,098 + CA265_RS03740 0.51 +0.8 889,203 + CA265_RS03740 0.57 +1.8
Or see this region's nucleotide sequence