Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS02955 and CA265_RS02960 are separated by 121 nucleotides CA265_RS02960 and CA265_RS02965 are separated by 72 nucleotides CA265_RS02965 and CA265_RS02970 are separated by 99 nucleotides
CA265_RS02955: CA265_RS02955 - G-D-S-L family lipolytic protein, at 737,713 to 739,014
_RS02955
CA265_RS02960: CA265_RS02960 - tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA, at 739,136 to 740,050
_RS02960
CA265_RS02965: CA265_RS02965 - hypothetical protein, at 740,123 to 740,770
_RS02965
CA265_RS02970: CA265_RS02970 - tRNA nucleotidyltransferase, at 740,870 to 742,324
_RS02970
Position (kb)
739
740
741 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 738.219 kb on + strand, within CA265_RS02955 at 738.241 kb on - strand, within CA265_RS02955 at 738.285 kb on + strand, within CA265_RS02955 at 738.286 kb on - strand, within CA265_RS02955 at 738.286 kb on - strand, within CA265_RS02955 at 738.293 kb on + strand, within CA265_RS02955 at 738.309 kb on - strand, within CA265_RS02955 at 738.327 kb on - strand, within CA265_RS02955 at 738.331 kb on - strand, within CA265_RS02955 at 738.364 kb on - strand, within CA265_RS02955 at 738.377 kb on + strand, within CA265_RS02955 at 738.378 kb on - strand, within CA265_RS02955 at 738.407 kb on + strand, within CA265_RS02955 at 738.408 kb on - strand, within CA265_RS02955 at 738.426 kb on - strand, within CA265_RS02955 at 738.428 kb on + strand, within CA265_RS02955 at 738.428 kb on + strand, within CA265_RS02955 at 738.428 kb on + strand, within CA265_RS02955 at 738.429 kb on - strand, within CA265_RS02955 at 738.465 kb on + strand, within CA265_RS02955 at 738.491 kb on + strand, within CA265_RS02955 at 738.491 kb on + strand at 738.532 kb on + strand, within CA265_RS02955 at 738.532 kb on + strand, within CA265_RS02955 at 738.557 kb on + strand, within CA265_RS02955 at 738.650 kb on + strand, within CA265_RS02955 at 738.731 kb on - strand, within CA265_RS02955 at 738.739 kb on - strand, within CA265_RS02955 at 738.783 kb on + strand, within CA265_RS02955 at 738.788 kb on - strand, within CA265_RS02955 at 738.789 kb on + strand, within CA265_RS02955 at 738.790 kb on - strand, within CA265_RS02955 at 738.838 kb on - strand, within CA265_RS02955 at 738.840 kb on + strand, within CA265_RS02955 at 738.876 kb on - strand, within CA265_RS02955 at 738.928 kb on - strand at 738.928 kb on - strand at 738.946 kb on - strand at 738.949 kb on + strand at 738.958 kb on - strand at 738.958 kb on - strand at 738.976 kb on - strand at 738.986 kb on - strand at 738.995 kb on + strand at 739.012 kb on + strand at 739.013 kb on - strand at 739.032 kb on + strand at 739.046 kb on - strand at 739.124 kb on + strand at 739.125 kb on - strand at 739.328 kb on - strand, within CA265_RS02960 at 739.353 kb on + strand, within CA265_RS02960 at 739.388 kb on + strand, within CA265_RS02960 at 739.437 kb on + strand, within CA265_RS02960 at 739.440 kb on - strand, within CA265_RS02960 at 739.480 kb on + strand, within CA265_RS02960 at 739.545 kb on - strand, within CA265_RS02960 at 739.599 kb on + strand, within CA265_RS02960 at 739.641 kb on - strand, within CA265_RS02960 at 739.730 kb on + strand, within CA265_RS02960 at 739.734 kb on + strand, within CA265_RS02960 at 739.756 kb on + strand, within CA265_RS02960 at 739.881 kb on - strand, within CA265_RS02960 at 740.110 kb on - strand at 740.120 kb on + strand at 740.120 kb on + strand at 740.430 kb on - strand, within CA265_RS02965 at 740.479 kb on + strand, within CA265_RS02965 at 740.480 kb on - strand, within CA265_RS02965 at 740.488 kb on - strand, within CA265_RS02965 at 740.488 kb on - strand, within CA265_RS02965 at 740.515 kb on + strand, within CA265_RS02965 at 740.516 kb on - strand, within CA265_RS02965 at 740.516 kb on - strand, within CA265_RS02965 at 740.608 kb on + strand, within CA265_RS02965 at 740.619 kb on - strand, within CA265_RS02965 at 740.619 kb on - strand, within CA265_RS02965 at 740.648 kb on - strand, within CA265_RS02965 at 740.648 kb on - strand, within CA265_RS02965 at 740.669 kb on - strand, within CA265_RS02965 at 740.753 kb on - strand at 740.807 kb on - strand at 740.833 kb on + strand at 740.866 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 738,219 + CA265_RS02955 0.39 +0.9 738,241 - CA265_RS02955 0.41 -2.0 738,285 + CA265_RS02955 0.44 -0.6 738,286 - CA265_RS02955 0.44 +0.3 738,286 - CA265_RS02955 0.44 +1.0 738,293 + CA265_RS02955 0.45 +0.5 738,309 - CA265_RS02955 0.46 -0.1 738,327 - CA265_RS02955 0.47 -0.7 738,331 - CA265_RS02955 0.47 +0.5 738,364 - CA265_RS02955 0.50 -0.1 738,377 + CA265_RS02955 0.51 +0.4 738,378 - CA265_RS02955 0.51 -0.0 738,407 + CA265_RS02955 0.53 -0.3 738,408 - CA265_RS02955 0.53 -0.5 738,426 - CA265_RS02955 0.55 -0.0 738,428 + CA265_RS02955 0.55 +0.8 738,428 + CA265_RS02955 0.55 +1.7 738,428 + CA265_RS02955 0.55 -0.6 738,429 - CA265_RS02955 0.55 +0.1 738,465 + CA265_RS02955 0.58 -1.2 738,491 + CA265_RS02955 0.60 -0.6 738,491 + +1.2 738,532 + CA265_RS02955 0.63 -2.1 738,532 + CA265_RS02955 0.63 -0.6 738,557 + CA265_RS02955 0.65 -0.4 738,650 + CA265_RS02955 0.72 -0.6 738,731 - CA265_RS02955 0.78 +0.2 738,739 - CA265_RS02955 0.79 -0.3 738,783 + CA265_RS02955 0.82 -0.0 738,788 - CA265_RS02955 0.83 -0.2 738,789 + CA265_RS02955 0.83 -0.6 738,790 - CA265_RS02955 0.83 -1.1 738,838 - CA265_RS02955 0.86 +0.6 738,840 + CA265_RS02955 0.87 -0.3 738,876 - CA265_RS02955 0.89 -1.4 738,928 - +0.2 738,928 - -0.1 738,946 - -0.1 738,949 + -0.3 738,958 - -0.6 738,958 - -0.3 738,976 - -0.6 738,986 - +0.1 738,995 + -0.3 739,012 + -0.4 739,013 - +1.7 739,032 + +1.3 739,046 - +1.2 739,124 + -0.3 739,125 - +0.7 739,328 - CA265_RS02960 0.21 +0.7 739,353 + CA265_RS02960 0.24 -0.3 739,388 + CA265_RS02960 0.28 -3.2 739,437 + CA265_RS02960 0.33 -0.8 739,440 - CA265_RS02960 0.33 -0.8 739,480 + CA265_RS02960 0.38 -2.6 739,545 - CA265_RS02960 0.45 -0.3 739,599 + CA265_RS02960 0.51 -2.7 739,641 - CA265_RS02960 0.55 -1.1 739,730 + CA265_RS02960 0.65 +0.0 739,734 + CA265_RS02960 0.65 -0.1 739,756 + CA265_RS02960 0.68 -1.0 739,881 - CA265_RS02960 0.81 -1.4 740,110 - -0.8 740,120 + -2.4 740,120 + +0.3 740,430 - CA265_RS02965 0.47 +1.0 740,479 + CA265_RS02965 0.55 -1.2 740,480 - CA265_RS02965 0.55 +1.1 740,488 - CA265_RS02965 0.56 +0.4 740,488 - CA265_RS02965 0.56 +0.9 740,515 + CA265_RS02965 0.60 +0.5 740,516 - CA265_RS02965 0.61 +0.3 740,516 - CA265_RS02965 0.61 -0.2 740,608 + CA265_RS02965 0.75 +0.1 740,619 - CA265_RS02965 0.77 +0.6 740,619 - CA265_RS02965 0.77 +1.0 740,648 - CA265_RS02965 0.81 +0.3 740,648 - CA265_RS02965 0.81 +0.8 740,669 - CA265_RS02965 0.84 +0.5 740,753 - +0.2 740,807 - -0.1 740,833 + -1.5 740,866 + +0.8
Or see this region's nucleotide sequence