Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35955 and BPHYT_RS35960 are separated by 876 nucleotides BPHYT_RS35960 and BPHYT_RS35965 are separated by 44 nucleotides BPHYT_RS35965 and BPHYT_RS35970 are separated by 33 nucleotides
BPHYT_RS35955: BPHYT_RS35955 - hypothetical protein, at 24,367 to 24,672
_RS35955
BPHYT_RS35960: BPHYT_RS35960 - ATPase, at 25,549 to 27,171
_RS35960
BPHYT_RS35965: BPHYT_RS35965 - hypothetical protein, at 27,216 to 27,434
_RS35965
BPHYT_RS35970: BPHYT_RS35970 - DNA recombinase, at 27,468 to 28,574
_RS35970
Position (kb) 25
26
27
28 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 24.671 kb on - strand at 24.720 kb on - strand at 24.820 kb on + strand at 25.081 kb on + strand at 25.081 kb on + strand at 25.259 kb on + strand at 25.308 kb on + strand at 25.309 kb on - strand at 25.451 kb on + strand at 25.470 kb on - strand at 25.494 kb on - strand at 25.543 kb on + strand at 25.812 kb on + strand, within BPHYT_RS35960 at 25.872 kb on + strand, within BPHYT_RS35960 at 25.873 kb on - strand, within BPHYT_RS35960 at 25.981 kb on - strand, within BPHYT_RS35960 at 26.011 kb on + strand, within BPHYT_RS35960 at 26.235 kb on + strand, within BPHYT_RS35960 at 26.441 kb on + strand, within BPHYT_RS35960 at 26.735 kb on + strand, within BPHYT_RS35960 at 26.820 kb on - strand, within BPHYT_RS35960 at 27.027 kb on + strand at 27.062 kb on + strand at 27.063 kb on - strand at 27.071 kb on + strand at 27.071 kb on + strand at 27.159 kb on + strand at 27.160 kb on - strand at 27.160 kb on - strand at 27.160 kb on - strand at 27.160 kb on - strand at 27.160 kb on - strand at 27.218 kb on - strand at 27.229 kb on + strand at 27.229 kb on + strand at 27.230 kb on - strand at 27.230 kb on - strand at 27.234 kb on + strand at 27.235 kb on - strand at 27.260 kb on - strand, within BPHYT_RS35965 at 27.347 kb on + strand, within BPHYT_RS35965 at 27.368 kb on + strand, within BPHYT_RS35965 at 27.368 kb on + strand, within BPHYT_RS35965 at 27.371 kb on - strand, within BPHYT_RS35965 at 27.378 kb on + strand, within BPHYT_RS35965 at 27.379 kb on - strand, within BPHYT_RS35965 at 27.489 kb on + strand at 27.489 kb on + strand at 27.490 kb on - strand at 27.490 kb on - strand at 27.509 kb on - strand at 27.788 kb on + strand, within BPHYT_RS35970 at 27.788 kb on + strand, within BPHYT_RS35970 at 27.789 kb on - strand, within BPHYT_RS35970 at 27.789 kb on - strand, within BPHYT_RS35970 at 27.816 kb on - strand, within BPHYT_RS35970 at 27.816 kb on - strand, within BPHYT_RS35970 at 27.850 kb on - strand, within BPHYT_RS35970 at 27.858 kb on + strand, within BPHYT_RS35970 at 27.859 kb on - strand, within BPHYT_RS35970 at 27.859 kb on - strand, within BPHYT_RS35970 at 27.974 kb on + strand, within BPHYT_RS35970 at 27.975 kb on - strand, within BPHYT_RS35970 at 28.003 kb on - strand, within BPHYT_RS35970
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 24,671 - +0.5 24,720 - -0.1 24,820 + +0.9 25,081 + +1.2 25,081 + +0.2 25,259 + +1.0 25,308 + -0.6 25,309 - +0.5 25,451 + +0.1 25,470 - -0.4 25,494 - -0.3 25,543 + -0.2 25,812 + BPHYT_RS35960 0.16 +0.8 25,872 + BPHYT_RS35960 0.20 +0.4 25,873 - BPHYT_RS35960 0.20 +0.3 25,981 - BPHYT_RS35960 0.27 +1.3 26,011 + BPHYT_RS35960 0.28 -0.3 26,235 + BPHYT_RS35960 0.42 -0.3 26,441 + BPHYT_RS35960 0.55 -0.2 26,735 + BPHYT_RS35960 0.73 +0.7 26,820 - BPHYT_RS35960 0.78 +0.2 27,027 + +1.3 27,062 + +1.4 27,063 - -1.1 27,071 + -3.8 27,071 + -0.0 27,159 + -0.0 27,160 - +1.5 27,160 - -0.8 27,160 - -0.6 27,160 - -0.1 27,160 - +0.0 27,218 - +1.3 27,229 + -0.0 27,229 + -1.9 27,230 - -0.8 27,230 - +1.0 27,234 + -0.2 27,235 - +0.9 27,260 - BPHYT_RS35965 0.20 -0.1 27,347 + BPHYT_RS35965 0.60 -0.3 27,368 + BPHYT_RS35965 0.69 -0.3 27,368 + BPHYT_RS35965 0.69 -0.8 27,371 - BPHYT_RS35965 0.71 +0.1 27,378 + BPHYT_RS35965 0.74 -0.1 27,379 - BPHYT_RS35965 0.74 +0.5 27,489 + -0.5 27,489 + +0.9 27,490 - +0.6 27,490 - +0.1 27,509 - +0.3 27,788 + BPHYT_RS35970 0.29 -0.3 27,788 + BPHYT_RS35970 0.29 +1.1 27,789 - BPHYT_RS35970 0.29 +0.1 27,789 - BPHYT_RS35970 0.29 +0.7 27,816 - BPHYT_RS35970 0.31 +1.2 27,816 - BPHYT_RS35970 0.31 -0.2 27,850 - BPHYT_RS35970 0.35 +0.6 27,858 + BPHYT_RS35970 0.35 -1.0 27,859 - BPHYT_RS35970 0.35 +0.1 27,859 - BPHYT_RS35970 0.35 +0.1 27,974 + BPHYT_RS35970 0.46 +0.2 27,975 - BPHYT_RS35970 0.46 +0.2 28,003 - BPHYT_RS35970 0.48 -0.0
Or see this region's nucleotide sequence