Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34125 and BPHYT_RS34130 are separated by 213 nucleotides BPHYT_RS34130 and BPHYT_RS34135 are separated by 84 nucleotides BPHYT_RS34135 and BPHYT_RS34140 are separated by 219 nucleotides
BPHYT_RS34125: BPHYT_RS34125 - heavy metal translocating P-type ATPase, at 3,257,619 to 3,260,237
_RS34125
BPHYT_RS34130: BPHYT_RS34130 - chemotaxis protein, at 3,260,451 to 3,262,424
_RS34130
BPHYT_RS34135: BPHYT_RS34135 - glutathione S-transferase, at 3,262,509 to 3,263,162
_RS34135
BPHYT_RS34140: BPHYT_RS34140 - poly(3-hydroxybutyrate) depolymerase, at 3,263,382 to 3,264,845
_RS34140
Position (kb)
3260
3261
3262
3263 Strain fitness (log2 ratio)
-1
0
1
2 at 3259.988 kb on + strand at 3259.988 kb on + strand at 3259.989 kb on - strand at 3259.989 kb on - strand at 3260.089 kb on - strand at 3260.401 kb on + strand at 3260.402 kb on - strand at 3260.434 kb on - strand at 3260.978 kb on + strand, within BPHYT_RS34130 at 3261.021 kb on + strand, within BPHYT_RS34130 at 3261.144 kb on + strand, within BPHYT_RS34130 at 3261.145 kb on - strand, within BPHYT_RS34130 at 3261.148 kb on - strand, within BPHYT_RS34130 at 3261.283 kb on - strand, within BPHYT_RS34130 at 3261.684 kb on - strand, within BPHYT_RS34130 at 3261.684 kb on - strand, within BPHYT_RS34130 at 3261.726 kb on - strand, within BPHYT_RS34130 at 3261.983 kb on + strand, within BPHYT_RS34130 at 3262.232 kb on + strand at 3262.606 kb on + strand, within BPHYT_RS34135 at 3262.639 kb on + strand, within BPHYT_RS34135 at 3262.639 kb on + strand, within BPHYT_RS34135 at 3262.639 kb on + strand, within BPHYT_RS34135 at 3262.660 kb on - strand, within BPHYT_RS34135 at 3262.669 kb on + strand, within BPHYT_RS34135 at 3262.669 kb on + strand, within BPHYT_RS34135 at 3262.896 kb on - strand, within BPHYT_RS34135 at 3263.125 kb on + strand at 3263.153 kb on + strand at 3263.281 kb on + strand at 3263.282 kb on - strand at 3263.303 kb on + strand at 3263.316 kb on - strand at 3263.343 kb on + strand at 3263.406 kb on + strand at 3263.407 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,259,988 + +0.0 3,259,988 + +0.6 3,259,989 - -1.1 3,259,989 - -1.0 3,260,089 - +1.1 3,260,401 + -0.2 3,260,402 - -0.9 3,260,434 - -0.1 3,260,978 + BPHYT_RS34130 0.27 -0.2 3,261,021 + BPHYT_RS34130 0.29 +0.1 3,261,144 + BPHYT_RS34130 0.35 -0.1 3,261,145 - BPHYT_RS34130 0.35 -0.4 3,261,148 - BPHYT_RS34130 0.35 +0.5 3,261,283 - BPHYT_RS34130 0.42 -0.4 3,261,684 - BPHYT_RS34130 0.62 +0.6 3,261,684 - BPHYT_RS34130 0.62 -0.6 3,261,726 - BPHYT_RS34130 0.65 -0.5 3,261,983 + BPHYT_RS34130 0.78 +1.5 3,262,232 + -0.6 3,262,606 + BPHYT_RS34135 0.15 -1.6 3,262,639 + BPHYT_RS34135 0.20 +0.4 3,262,639 + BPHYT_RS34135 0.20 +0.5 3,262,639 + BPHYT_RS34135 0.20 -0.4 3,262,660 - BPHYT_RS34135 0.23 -0.1 3,262,669 + BPHYT_RS34135 0.24 +1.0 3,262,669 + BPHYT_RS34135 0.24 +0.4 3,262,896 - BPHYT_RS34135 0.59 +2.0 3,263,125 + -0.3 3,263,153 + +0.5 3,263,281 + -0.7 3,263,282 - -0.1 3,263,303 + +1.5 3,263,316 - -1.4 3,263,343 + -0.5 3,263,406 + +0.1 3,263,407 - +0.1
Or see this region's nucleotide sequence