Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS31685

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS31680 and BPHYT_RS31685 are separated by 57 nucleotidesBPHYT_RS31685 and BPHYT_RS31690 are separated by 23 nucleotides BPHYT_RS31680: BPHYT_RS31680 - dehydrogenase, at 2,662,951 to 2,663,844 _RS31680 BPHYT_RS31685: BPHYT_RS31685 - D-isomer specific 2-hydroxyacid dehydrogenase, at 2,663,902 to 2,664,882 _RS31685 BPHYT_RS31690: BPHYT_RS31690 - hypothetical protein, at 2,664,906 to 2,666,057 _RS31690 Position (kb) 2663 2664 2665Strain fitness (log2 ratio) -2 -1 0 1at 2662.923 kb on - strandat 2662.938 kb on - strandat 2662.940 kb on + strandat 2662.944 kb on + strandat 2663.263 kb on - strand, within BPHYT_RS31680at 2663.354 kb on + strand, within BPHYT_RS31680at 2663.378 kb on + strand, within BPHYT_RS31680at 2663.527 kb on - strand, within BPHYT_RS31680at 2663.647 kb on - strand, within BPHYT_RS31680at 2663.844 kb on + strandat 2664.285 kb on - strand, within BPHYT_RS31685at 2664.307 kb on - strand, within BPHYT_RS31685at 2664.582 kb on + strand, within BPHYT_RS31685at 2665.691 kb on - strand, within BPHYT_RS31690at 2665.699 kb on + strand, within BPHYT_RS31690at 2665.870 kb on + strand, within BPHYT_RS31690at 2665.871 kb on - strand, within BPHYT_RS31690

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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2,662,923 - -1.8
2,662,938 - -0.0
2,662,940 + +1.1
2,662,944 + -1.0
2,663,263 - BPHYT_RS31680 0.35 -1.4
2,663,354 + BPHYT_RS31680 0.45 +0.1
2,663,378 + BPHYT_RS31680 0.48 -0.5
2,663,527 - BPHYT_RS31680 0.64 +0.6
2,663,647 - BPHYT_RS31680 0.78 -0.5
2,663,844 + +0.6
2,664,285 - BPHYT_RS31685 0.39 +0.2
2,664,307 - BPHYT_RS31685 0.41 +1.5
2,664,582 + BPHYT_RS31685 0.69 +0.7
2,665,691 - BPHYT_RS31690 0.68 +0.1
2,665,699 + BPHYT_RS31690 0.69 +0.7
2,665,870 + BPHYT_RS31690 0.84 -0.6
2,665,871 - BPHYT_RS31690 0.84 +1.1

Or see this region's nucleotide sequence