Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS31610

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS31605 and BPHYT_RS31610 are separated by 56 nucleotides BPHYT_RS31605: BPHYT_RS31605 - glucoamylase, at 2,641,395 to 2,643,185 _RS31605 BPHYT_RS31610: BPHYT_RS31610 - short-chain dehydrogenase, at 2,643,242 to 2,644,240 _RS31610 Position (kb) 2643 2644 2645Strain fitness (log2 ratio) -1 0 1 2at 2642.272 kb on + strand, within BPHYT_RS31605at 2642.273 kb on - strand, within BPHYT_RS31605at 2642.884 kb on - strand, within BPHYT_RS31605at 2643.100 kb on - strandat 2643.244 kb on - strandat 2643.452 kb on - strand, within BPHYT_RS31610at 2643.452 kb on - strand, within BPHYT_RS31610at 2644.241 kb on - strandat 2644.317 kb on + strandat 2644.709 kb on + strandat 2644.763 kb on - strandat 2644.852 kb on + strandat 2644.961 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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2,642,272 + BPHYT_RS31605 0.49 +0.2
2,642,273 - BPHYT_RS31605 0.49 -0.1
2,642,884 - BPHYT_RS31605 0.83 +0.0
2,643,100 - -0.5
2,643,244 - -1.4
2,643,452 - BPHYT_RS31610 0.21 -0.7
2,643,452 - BPHYT_RS31610 0.21 +0.8
2,644,241 - +1.8
2,644,317 + +0.1
2,644,709 + -0.2
2,644,763 - +0.9
2,644,852 + -1.6
2,644,961 - -0.4

Or see this region's nucleotide sequence