Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS29260

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS29255 and BPHYT_RS29260 are separated by 52 nucleotidesBPHYT_RS29260 and BPHYT_RS29265 are separated by 550 nucleotides BPHYT_RS29255: BPHYT_RS29255 - aspartate carbamoyltransferase, at 2,119,598 to 2,120,890 _RS29255 BPHYT_RS29260: BPHYT_RS29260 - esterase, at 2,120,943 to 2,121,794 _RS29260 BPHYT_RS29265: BPHYT_RS29265 - AsnC family transcriptional regulator, at 2,122,345 to 2,122,836 _RS29265 Position (kb) 2120 2121 2122Strain fitness (log2 ratio) -1 0 1 2 3at 2120.118 kb on - strand, within BPHYT_RS29255at 2120.329 kb on + strand, within BPHYT_RS29255at 2120.450 kb on + strand, within BPHYT_RS29255at 2120.631 kb on + strand, within BPHYT_RS29255at 2120.632 kb on - strand, within BPHYT_RS29255at 2120.849 kb on + strandat 2121.107 kb on + strand, within BPHYT_RS29260at 2121.107 kb on + strand, within BPHYT_RS29260at 2121.493 kb on + strand, within BPHYT_RS29260at 2121.528 kb on + strand, within BPHYT_RS29260at 2121.586 kb on + strand, within BPHYT_RS29260at 2121.587 kb on - strand, within BPHYT_RS29260at 2121.908 kb on + strandat 2121.954 kb on + strandat 2122.036 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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2,120,118 - BPHYT_RS29255 0.40 +0.0
2,120,329 + BPHYT_RS29255 0.57 +0.4
2,120,450 + BPHYT_RS29255 0.66 -0.5
2,120,631 + BPHYT_RS29255 0.80 -0.5
2,120,632 - BPHYT_RS29255 0.80 +3.1
2,120,849 + +0.1
2,121,107 + BPHYT_RS29260 0.19 +1.7
2,121,107 + BPHYT_RS29260 0.19 +1.2
2,121,493 + BPHYT_RS29260 0.65 +0.3
2,121,528 + BPHYT_RS29260 0.69 +1.5
2,121,586 + BPHYT_RS29260 0.75 +0.8
2,121,587 - BPHYT_RS29260 0.76 +2.3
2,121,908 + +0.1
2,121,954 + -1.4
2,122,036 + +1.4

Or see this region's nucleotide sequence