Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28270

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28265 and BPHYT_RS28270 are separated by 33 nucleotidesBPHYT_RS28270 and BPHYT_RS28275 are separated by 134 nucleotides BPHYT_RS28265: BPHYT_RS28265 - hypothetical protein, at 1,897,872 to 1,898,879 _RS28265 BPHYT_RS28270: BPHYT_RS28270 - ABC transporter substrate-binding protein, at 1,898,913 to 1,900,034 _RS28270 BPHYT_RS28275: BPHYT_RS28275 - glycerol acyltransferase, at 1,900,169 to 1,900,879 _RS28275 Position (kb) 1898 1899 1900 1901Strain fitness (log2 ratio) -1 0 1at 1898.209 kb on + strand, within BPHYT_RS28265at 1898.377 kb on - strand, within BPHYT_RS28265at 1898.377 kb on - strand, within BPHYT_RS28265at 1898.377 kb on - strand, within BPHYT_RS28265at 1898.618 kb on + strand, within BPHYT_RS28265at 1898.621 kb on - strand, within BPHYT_RS28265at 1898.621 kb on - strand, within BPHYT_RS28265at 1898.696 kb on + strand, within BPHYT_RS28265at 1898.923 kb on + strandat 1898.953 kb on + strandat 1898.954 kb on - strandat 1899.002 kb on - strandat 1899.002 kb on - strandat 1899.116 kb on - strand, within BPHYT_RS28270at 1899.226 kb on + strand, within BPHYT_RS28270at 1899.691 kb on + strand, within BPHYT_RS28270at 1899.728 kb on - strand, within BPHYT_RS28270at 1900.070 kb on - strandat 1900.273 kb on + strand, within BPHYT_RS28275at 1900.274 kb on - strand, within BPHYT_RS28275at 1900.274 kb on - strand, within BPHYT_RS28275at 1900.850 kb on - strandat 1900.950 kb on + strandat 1900.951 kb on - strandat 1900.951 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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1,898,209 + BPHYT_RS28265 0.33 -0.2
1,898,377 - BPHYT_RS28265 0.50 +0.2
1,898,377 - BPHYT_RS28265 0.50 -1.4
1,898,377 - BPHYT_RS28265 0.50 -0.4
1,898,618 + BPHYT_RS28265 0.74 -0.5
1,898,621 - BPHYT_RS28265 0.74 +0.6
1,898,621 - BPHYT_RS28265 0.74 +0.1
1,898,696 + BPHYT_RS28265 0.82 +0.6
1,898,923 + +0.3
1,898,953 + +0.4
1,898,954 - -0.2
1,899,002 - +0.2
1,899,002 - -0.3
1,899,116 - BPHYT_RS28270 0.18 +1.5
1,899,226 + BPHYT_RS28270 0.28 -0.1
1,899,691 + BPHYT_RS28270 0.69 +0.1
1,899,728 - BPHYT_RS28270 0.73 +0.9
1,900,070 - +0.7
1,900,273 + BPHYT_RS28275 0.15 +0.1
1,900,274 - BPHYT_RS28275 0.15 -0.9
1,900,274 - BPHYT_RS28275 0.15 +0.6
1,900,850 - -0.3
1,900,950 + -0.3
1,900,951 - -0.7
1,900,951 - +1.6

Or see this region's nucleotide sequence