Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26920 and BPHYT_RS26925 are separated by 140 nucleotides BPHYT_RS26925 and BPHYT_RS26930 are separated by 352 nucleotides BPHYT_RS26930 and BPHYT_RS26935 are separated by 123 nucleotides
BPHYT_RS26920: BPHYT_RS26920 - major facilitator transporter, at 1,613,277 to 1,615,028
_RS26920
BPHYT_RS26925: BPHYT_RS26925 - NIPSNAP family containing protein, at 1,615,169 to 1,615,519
_RS26925
BPHYT_RS26930: BPHYT_RS26930 - mechanosensitive ion channel protein, at 1,615,872 to 1,618,541
_RS26930
BPHYT_RS26935: BPHYT_RS26935 - ATP-dependent DNA helicase, at 1,618,665 to 1,620,929
_RS26935
Position (kb)
1615
1616
1617
1618
1619 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1615.072 kb on + strand at 1615.110 kb on + strand at 1615.111 kb on - strand at 1615.114 kb on + strand at 1615.114 kb on + strand at 1615.115 kb on - strand at 1615.115 kb on - strand at 1615.178 kb on - strand at 1615.256 kb on - strand, within BPHYT_RS26925 at 1615.310 kb on - strand, within BPHYT_RS26925 at 1615.355 kb on + strand, within BPHYT_RS26925 at 1615.365 kb on - strand, within BPHYT_RS26925 at 1615.871 kb on + strand at 1616.737 kb on + strand, within BPHYT_RS26930 at 1616.829 kb on + strand, within BPHYT_RS26930 at 1616.830 kb on - strand, within BPHYT_RS26930 at 1616.830 kb on - strand, within BPHYT_RS26930 at 1616.830 kb on - strand, within BPHYT_RS26930 at 1616.830 kb on - strand, within BPHYT_RS26930 at 1616.830 kb on - strand, within BPHYT_RS26930 at 1617.396 kb on + strand, within BPHYT_RS26930 at 1617.637 kb on + strand, within BPHYT_RS26930 at 1617.771 kb on - strand, within BPHYT_RS26930 at 1617.771 kb on - strand, within BPHYT_RS26930 at 1617.771 kb on - strand, within BPHYT_RS26930 at 1617.954 kb on + strand, within BPHYT_RS26930 at 1618.544 kb on + strand at 1618.807 kb on + strand at 1618.813 kb on + strand at 1618.813 kb on + strand at 1618.813 kb on + strand at 1618.813 kb on + strand at 1618.814 kb on - strand at 1618.814 kb on - strand at 1618.820 kb on - strand at 1618.820 kb on - strand at 1619.138 kb on - strand, within BPHYT_RS26935 at 1619.138 kb on - strand, within BPHYT_RS26935
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 1,615,072 + +2.1 1,615,110 + +1.1 1,615,111 - +1.5 1,615,114 + +1.5 1,615,114 + +0.7 1,615,115 - -0.6 1,615,115 - +0.7 1,615,178 - +0.4 1,615,256 - BPHYT_RS26925 0.25 -0.1 1,615,310 - BPHYT_RS26925 0.40 +0.5 1,615,355 + BPHYT_RS26925 0.53 +0.6 1,615,365 - BPHYT_RS26925 0.56 +1.0 1,615,871 + +0.6 1,616,737 + BPHYT_RS26930 0.32 -0.7 1,616,829 + BPHYT_RS26930 0.36 +0.2 1,616,830 - BPHYT_RS26930 0.36 +0.7 1,616,830 - BPHYT_RS26930 0.36 +0.8 1,616,830 - BPHYT_RS26930 0.36 -0.1 1,616,830 - BPHYT_RS26930 0.36 +3.5 1,616,830 - BPHYT_RS26930 0.36 +0.6 1,617,396 + BPHYT_RS26930 0.57 -0.4 1,617,637 + BPHYT_RS26930 0.66 +1.1 1,617,771 - BPHYT_RS26930 0.71 +0.6 1,617,771 - BPHYT_RS26930 0.71 +1.6 1,617,771 - BPHYT_RS26930 0.71 +0.7 1,617,954 + BPHYT_RS26930 0.78 +0.7 1,618,544 + +1.2 1,618,807 + +0.7 1,618,813 + -0.1 1,618,813 + +0.6 1,618,813 + +1.2 1,618,813 + +0.4 1,618,814 - -0.2 1,618,814 - +1.2 1,618,820 - +0.6 1,618,820 - -0.9 1,619,138 - BPHYT_RS26935 0.21 -0.6 1,619,138 - BPHYT_RS26935 0.21 -1.7
Or see this region's nucleotide sequence