Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS26320

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS26310 and BPHYT_RS26315 are separated by 135 nucleotidesBPHYT_RS26315 and BPHYT_RS26320 are separated by 4 nucleotidesBPHYT_RS26320 and BPHYT_RS26325 are separated by 121 nucleotides BPHYT_RS26310: BPHYT_RS26310 - ribonuclease R, at 1,474,412 to 1,475,314 _RS26310 BPHYT_RS26315: BPHYT_RS26315 - sigma factor sigB regulation protein rsbQ, at 1,475,450 to 1,476,253 _RS26315 BPHYT_RS26320: BPHYT_RS26320 - ATPase, at 1,476,258 to 1,478,588 _RS26320 BPHYT_RS26325: BPHYT_RS26325 - ATPase, at 1,478,710 to 1,479,813 _RS26325 Position (kb) 1476 1477 1478 1479Strain fitness (log2 ratio) -1 0 1at 1475.373 kb on - strandat 1475.576 kb on + strand, within BPHYT_RS26315at 1475.816 kb on + strand, within BPHYT_RS26315at 1475.834 kb on + strand, within BPHYT_RS26315at 1475.895 kb on - strand, within BPHYT_RS26315at 1475.895 kb on - strand, within BPHYT_RS26315at 1476.252 kb on - strandat 1476.252 kb on - strandat 1476.592 kb on - strand, within BPHYT_RS26320at 1476.634 kb on - strandat 1476.634 kb on - strandat 1476.634 kb on - strand, within BPHYT_RS26320at 1476.634 kb on - strand, within BPHYT_RS26320at 1477.401 kb on + strand, within BPHYT_RS26320at 1477.825 kb on - strand, within BPHYT_RS26320at 1478.375 kb on - strandat 1478.642 kb on + strandat 1478.864 kb on - strand, within BPHYT_RS26325at 1478.864 kb on - strand, within BPHYT_RS26325at 1478.864 kb on - strand, within BPHYT_RS26325at 1479.145 kb on - strand, within BPHYT_RS26325at 1479.145 kb on - strand, within BPHYT_RS26325at 1479.368 kb on - strand, within BPHYT_RS26325

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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1,475,373 - +0.1
1,475,576 + BPHYT_RS26315 0.16 +0.5
1,475,816 + BPHYT_RS26315 0.46 -0.9
1,475,834 + BPHYT_RS26315 0.48 +0.3
1,475,895 - BPHYT_RS26315 0.55 +1.0
1,475,895 - BPHYT_RS26315 0.55 +1.1
1,476,252 - +0.3
1,476,252 - +0.8
1,476,592 - BPHYT_RS26320 0.14 +0.2
1,476,634 - +1.3
1,476,634 - -0.7
1,476,634 - BPHYT_RS26320 0.16 -0.3
1,476,634 - BPHYT_RS26320 0.16 -0.1
1,477,401 + BPHYT_RS26320 0.49 +0.1
1,477,825 - BPHYT_RS26320 0.67 +1.5
1,478,375 - +1.3
1,478,642 + +0.6
1,478,864 - BPHYT_RS26325 0.14 -0.2
1,478,864 - BPHYT_RS26325 0.14 +0.6
1,478,864 - BPHYT_RS26325 0.14 +1.6
1,479,145 - BPHYT_RS26325 0.39 +0.9
1,479,145 - BPHYT_RS26325 0.39 +0.5
1,479,368 - BPHYT_RS26325 0.60 -0.7

Or see this region's nucleotide sequence