Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24200 and BPHYT_RS24205 are separated by 57 nucleotides BPHYT_RS24205 and BPHYT_RS24210 are separated by 91 nucleotides BPHYT_RS24210 and BPHYT_RS24215 are separated by 254 nucleotides BPHYT_RS24215 and BPHYT_RS24220 overlap by 26 nucleotides
BPHYT_RS24200: BPHYT_RS24200 - 4-oxalocrotonate tautomerase, at 996,956 to 997,141
_RS24200
BPHYT_RS24205: BPHYT_RS24205 - aldolase, at 997,199 to 997,975
_RS24205
BPHYT_RS24210: BPHYT_RS24210 - urea transporter, at 998,067 to 999,011
_RS24210
BPHYT_RS24215: BPHYT_RS24215 - transcriptional regulator, at 999,266 to 999,820
_RS24215
BPHYT_RS24220: BPHYT_RS24220 - alpha/beta hydrolase, at 999,795 to 1,000,760
_RS24220
Position (kb) 998
999
1000 Strain fitness (log2 ratio) -1
0
1
2 at 997.146 kb on + strand at 997.284 kb on + strand, within BPHYT_RS24205 at 997.284 kb on + strand, within BPHYT_RS24205 at 997.843 kb on - strand, within BPHYT_RS24205 at 998.068 kb on + strand at 998.069 kb on - strand at 998.169 kb on - strand, within BPHYT_RS24210 at 998.816 kb on - strand, within BPHYT_RS24210 at 998.816 kb on - strand, within BPHYT_RS24210 at 999.103 kb on - strand at 999.387 kb on - strand, within BPHYT_RS24215 at 999.398 kb on + strand, within BPHYT_RS24215 at 999.399 kb on - strand, within BPHYT_RS24215 at 999.435 kb on - strand, within BPHYT_RS24215 at 999.641 kb on - strand, within BPHYT_RS24215 at 999.641 kb on - strand, within BPHYT_RS24215 at 999.642 kb on + strand, within BPHYT_RS24215 at 999.683 kb on + strand, within BPHYT_RS24215 at 999.683 kb on + strand, within BPHYT_RS24215 at 999.684 kb on - strand, within BPHYT_RS24215 at 999.998 kb on - strand, within BPHYT_RS24220
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 997,146 + -1.1 997,284 + BPHYT_RS24205 0.11 -0.5 997,284 + BPHYT_RS24205 0.11 -0.1 997,843 - BPHYT_RS24205 0.83 +1.2 998,068 + +0.3 998,069 - -1.3 998,169 - BPHYT_RS24210 0.11 +0.1 998,816 - BPHYT_RS24210 0.79 +1.9 998,816 - BPHYT_RS24210 0.79 +0.3 999,103 - -1.1 999,387 - BPHYT_RS24215 0.22 +1.1 999,398 + BPHYT_RS24215 0.24 -0.2 999,399 - BPHYT_RS24215 0.24 +0.6 999,435 - BPHYT_RS24215 0.30 +0.6 999,641 - BPHYT_RS24215 0.68 +0.1 999,641 - BPHYT_RS24215 0.68 +0.0 999,642 + BPHYT_RS24215 0.68 -0.2 999,683 + BPHYT_RS24215 0.75 -0.9 999,683 + BPHYT_RS24215 0.75 +0.2 999,684 - BPHYT_RS24215 0.75 +0.2 999,998 - BPHYT_RS24220 0.21 -0.1
Or see this region's nucleotide sequence