Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22105 and BPHYT_RS22110 are separated by 316 nucleotides BPHYT_RS22110 and BPHYT_RS22115 are separated by 30 nucleotides BPHYT_RS22115 and BPHYT_RS22120 are separated by 161 nucleotides
BPHYT_RS22105: BPHYT_RS22105 - tRNA synthetase, at 517,568 to 518,107
_RS22105
BPHYT_RS22110: BPHYT_RS22110 - membrane protein, at 518,424 to 519,983
_RS22110
BPHYT_RS22115: BPHYT_RS22115 - histidine kinase, at 520,014 to 520,460
_RS22115
BPHYT_RS22120: BPHYT_RS22120 - sulfate transporter, at 520,622 to 522,070
_RS22120
Position (kb) 518
519
520 Strain fitness (log2 ratio) -2
-1
0
1
2 at 517.461 kb on - strand at 517.461 kb on - strand at 517.461 kb on - strand at 517.461 kb on - strand at 517.462 kb on + strand at 517.463 kb on - strand at 517.463 kb on - strand at 517.532 kb on + strand at 517.533 kb on - strand at 517.553 kb on + strand at 517.554 kb on - strand at 517.554 kb on - strand at 517.568 kb on - strand at 517.587 kb on + strand at 517.588 kb on - strand at 517.772 kb on + strand, within BPHYT_RS22105 at 518.052 kb on + strand, within BPHYT_RS22105 at 518.053 kb on - strand, within BPHYT_RS22105 at 518.053 kb on - strand, within BPHYT_RS22105 at 518.105 kb on + strand at 518.209 kb on - strand at 518.407 kb on + strand at 518.408 kb on - strand at 518.483 kb on + strand at 518.484 kb on - strand at 518.577 kb on + strand at 518.596 kb on - strand, within BPHYT_RS22110 at 518.694 kb on + strand, within BPHYT_RS22110 at 518.901 kb on + strand, within BPHYT_RS22110 at 518.902 kb on - strand, within BPHYT_RS22110 at 518.913 kb on + strand, within BPHYT_RS22110 at 518.914 kb on - strand, within BPHYT_RS22110 at 518.977 kb on - strand, within BPHYT_RS22110 at 518.994 kb on + strand, within BPHYT_RS22110 at 519.545 kb on + strand, within BPHYT_RS22110 at 519.545 kb on + strand, within BPHYT_RS22110 at 519.546 kb on - strand, within BPHYT_RS22110 at 519.717 kb on + strand, within BPHYT_RS22110 at 519.717 kb on + strand, within BPHYT_RS22110 at 519.993 kb on - strand at 520.493 kb on - strand at 520.498 kb on - strand at 520.649 kb on + strand at 520.685 kb on - strand at 520.688 kb on + strand at 520.689 kb on - strand at 520.866 kb on + strand, within BPHYT_RS22120
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 517,461 - +0.5 517,461 - +1.8 517,461 - +0.8 517,461 - +1.3 517,462 + -0.1 517,463 - -0.7 517,463 - +0.3 517,532 + -0.2 517,533 - +0.4 517,553 + +0.3 517,554 - -0.1 517,554 - -0.5 517,568 - -0.2 517,587 + +0.4 517,588 - +0.3 517,772 + BPHYT_RS22105 0.38 +0.5 518,052 + BPHYT_RS22105 0.90 +1.2 518,053 - BPHYT_RS22105 0.90 +0.4 518,053 - BPHYT_RS22105 0.90 +0.4 518,105 + -0.2 518,209 - -0.5 518,407 + -0.8 518,408 - +0.1 518,483 + +0.6 518,484 - -0.5 518,577 + +0.3 518,596 - BPHYT_RS22110 0.11 -0.5 518,694 + BPHYT_RS22110 0.17 -0.4 518,901 + BPHYT_RS22110 0.31 -0.1 518,902 - BPHYT_RS22110 0.31 +0.0 518,913 + BPHYT_RS22110 0.31 +0.4 518,914 - BPHYT_RS22110 0.31 +0.2 518,977 - BPHYT_RS22110 0.35 -0.1 518,994 + BPHYT_RS22110 0.37 -0.7 519,545 + BPHYT_RS22110 0.72 -0.1 519,545 + BPHYT_RS22110 0.72 -0.0 519,546 - BPHYT_RS22110 0.72 +0.1 519,717 + BPHYT_RS22110 0.83 +1.0 519,717 + BPHYT_RS22110 0.83 -0.4 519,993 - -0.8 520,493 - +0.6 520,498 - -0.3 520,649 + -2.3 520,685 - +0.2 520,688 + +0.4 520,689 - -0.4 520,866 + BPHYT_RS22120 0.17 +0.6
Or see this region's nucleotide sequence