Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22100 and BPHYT_RS22105 are separated by 273 nucleotides BPHYT_RS22105 and BPHYT_RS22110 are separated by 316 nucleotides
BPHYT_RS22100: BPHYT_RS22100 - ribonuclease BN, at 515,963 to 517,294
_RS22100
BPHYT_RS22105: BPHYT_RS22105 - tRNA synthetase, at 517,568 to 518,107
_RS22105
BPHYT_RS22110: BPHYT_RS22110 - membrane protein, at 518,424 to 519,983
_RS22110
Position (kb) 517
518
519 Strain fitness (log2 ratio) -1
0
1
2 at 516.608 kb on + strand, within BPHYT_RS22100 at 516.736 kb on - strand, within BPHYT_RS22100 at 516.916 kb on + strand, within BPHYT_RS22100 at 517.164 kb on + strand at 517.165 kb on - strand at 517.461 kb on - strand at 517.461 kb on - strand at 517.461 kb on - strand at 517.461 kb on - strand at 517.462 kb on + strand at 517.463 kb on - strand at 517.463 kb on - strand at 517.532 kb on + strand at 517.533 kb on - strand at 517.553 kb on + strand at 517.554 kb on - strand at 517.554 kb on - strand at 517.568 kb on - strand at 517.587 kb on + strand at 517.588 kb on - strand at 517.772 kb on + strand, within BPHYT_RS22105 at 518.052 kb on + strand, within BPHYT_RS22105 at 518.053 kb on - strand, within BPHYT_RS22105 at 518.053 kb on - strand, within BPHYT_RS22105 at 518.105 kb on + strand at 518.209 kb on - strand at 518.407 kb on + strand at 518.408 kb on - strand at 518.483 kb on + strand at 518.484 kb on - strand at 518.577 kb on + strand at 518.596 kb on - strand, within BPHYT_RS22110 at 518.694 kb on + strand, within BPHYT_RS22110 at 518.901 kb on + strand, within BPHYT_RS22110 at 518.902 kb on - strand, within BPHYT_RS22110 at 518.913 kb on + strand, within BPHYT_RS22110 at 518.914 kb on - strand, within BPHYT_RS22110 at 518.977 kb on - strand, within BPHYT_RS22110 at 518.994 kb on + strand, within BPHYT_RS22110
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 516,608 + BPHYT_RS22100 0.48 -0.2 516,736 - BPHYT_RS22100 0.58 +1.6 516,916 + BPHYT_RS22100 0.72 +1.1 517,164 + +0.1 517,165 - +0.2 517,461 - +0.5 517,461 - +1.8 517,461 - +0.8 517,461 - +1.3 517,462 + -0.1 517,463 - -0.7 517,463 - +0.3 517,532 + -0.2 517,533 - +0.4 517,553 + +0.3 517,554 - -0.1 517,554 - -0.5 517,568 - -0.2 517,587 + +0.4 517,588 - +0.3 517,772 + BPHYT_RS22105 0.38 +0.5 518,052 + BPHYT_RS22105 0.90 +1.2 518,053 - BPHYT_RS22105 0.90 +0.4 518,053 - BPHYT_RS22105 0.90 +0.4 518,105 + -0.2 518,209 - -0.5 518,407 + -0.8 518,408 - +0.1 518,483 + +0.6 518,484 - -0.5 518,577 + +0.3 518,596 - BPHYT_RS22110 0.11 -0.5 518,694 + BPHYT_RS22110 0.17 -0.4 518,901 + BPHYT_RS22110 0.31 -0.1 518,902 - BPHYT_RS22110 0.31 +0.0 518,913 + BPHYT_RS22110 0.31 +0.4 518,914 - BPHYT_RS22110 0.31 +0.2 518,977 - BPHYT_RS22110 0.35 -0.1 518,994 + BPHYT_RS22110 0.37 -0.7
Or see this region's nucleotide sequence