Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21930 and BPHYT_RS21935 are separated by 81 nucleotides BPHYT_RS21935 and BPHYT_RS21940 are separated by 165 nucleotides BPHYT_RS21940 and BPHYT_RS21945 are separated by 7 nucleotides
BPHYT_RS21930: BPHYT_RS21930 - MarR family transcriptional regulator, at 477,266 to 477,688
_RS21930
BPHYT_RS21935: BPHYT_RS21935 - MarR family transcriptional regulator, at 477,770 to 478,192
_RS21935
BPHYT_RS21940: BPHYT_RS21940 - hemolysin D, at 478,358 to 479,383
_RS21940
BPHYT_RS21945: BPHYT_RS21945 - DSBA oxidoreductase, at 479,391 to 480,962
_RS21945
Position (kb) 478
479
480 Strain fitness (log2 ratio) -1
0
1 at 477.835 kb on - strand, within BPHYT_RS21935 at 477.835 kb on - strand, within BPHYT_RS21935 at 477.835 kb on - strand, within BPHYT_RS21935 at 477.836 kb on + strand, within BPHYT_RS21935 at 478.274 kb on - strand at 478.292 kb on - strand at 478.455 kb on - strand at 478.745 kb on + strand, within BPHYT_RS21940 at 478.961 kb on + strand, within BPHYT_RS21940 at 478.992 kb on - strand, within BPHYT_RS21940 at 479.964 kb on + strand, within BPHYT_RS21945 at 479.964 kb on + strand, within BPHYT_RS21945 at 479.965 kb on - strand, within BPHYT_RS21945 at 480.029 kb on + strand, within BPHYT_RS21945
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 477,835 - BPHYT_RS21935 0.15 -0.7 477,835 - BPHYT_RS21935 0.15 -0.1 477,835 - BPHYT_RS21935 0.15 +0.2 477,836 + BPHYT_RS21935 0.16 -1.5 478,274 - +0.9 478,292 - -0.5 478,455 - -0.5 478,745 + BPHYT_RS21940 0.38 -1.1 478,961 + BPHYT_RS21940 0.59 -1.2 478,992 - BPHYT_RS21940 0.62 -1.2 479,964 + BPHYT_RS21945 0.36 -0.2 479,964 + BPHYT_RS21945 0.36 +0.1 479,965 - BPHYT_RS21945 0.37 -0.1 480,029 + BPHYT_RS21945 0.41 +0.0
Or see this region's nucleotide sequence