Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20285 and BPHYT_RS20290 are separated by 289 nucleotides BPHYT_RS20290 and BPHYT_RS20295 overlap by 4 nucleotides
BPHYT_RS20285: BPHYT_RS20285 - MFS transporter, at 109,875 to 111,341
_RS20285
BPHYT_RS20290: BPHYT_RS20290 - LysR family transcriptional regulator, at 111,631 to 112,569
_RS20290
BPHYT_RS20295: BPHYT_RS20295 - ligand-gated channel, at 112,566 to 114,749
_RS20295
Position (kb) 111
112
113 Strain fitness (log2 ratio) -2
-1
0
1 at 110.784 kb on - strand, within BPHYT_RS20285 at 110.942 kb on + strand, within BPHYT_RS20285 at 111.826 kb on + strand, within BPHYT_RS20290 at 111.829 kb on + strand, within BPHYT_RS20290 at 112.368 kb on - strand, within BPHYT_RS20290 at 112.621 kb on + strand at 112.621 kb on + strand at 112.625 kb on - strand at 112.792 kb on + strand, within BPHYT_RS20295 at 112.793 kb on - strand, within BPHYT_RS20295 at 112.793 kb on - strand, within BPHYT_RS20295 at 112.879 kb on + strand, within BPHYT_RS20295 at 113.051 kb on - strand, within BPHYT_RS20295 at 113.051 kb on - strand, within BPHYT_RS20295 at 113.056 kb on + strand, within BPHYT_RS20295 at 113.164 kb on + strand, within BPHYT_RS20295 at 113.215 kb on + strand, within BPHYT_RS20295 at 113.216 kb on - strand, within BPHYT_RS20295 at 113.216 kb on - strand, within BPHYT_RS20295 at 113.216 kb on - strand, within BPHYT_RS20295 at 113.225 kb on - strand, within BPHYT_RS20295 at 113.291 kb on - strand, within BPHYT_RS20295 at 113.321 kb on - strand, within BPHYT_RS20295 at 113.343 kb on - strand, within BPHYT_RS20295 at 113.379 kb on - strand, within BPHYT_RS20295 at 113.379 kb on - strand, within BPHYT_RS20295 at 113.379 kb on - strand, within BPHYT_RS20295 at 113.379 kb on - strand, within BPHYT_RS20295 at 113.509 kb on + strand, within BPHYT_RS20295 at 113.510 kb on - strand, within BPHYT_RS20295 at 113.539 kb on + strand, within BPHYT_RS20295
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 110,784 - BPHYT_RS20285 0.62 -0.2 110,942 + BPHYT_RS20285 0.73 +0.6 111,826 + BPHYT_RS20290 0.21 +0.3 111,829 + BPHYT_RS20290 0.21 +0.2 112,368 - BPHYT_RS20290 0.78 +1.4 112,621 + -1.6 112,621 + -0.5 112,625 - -0.7 112,792 + BPHYT_RS20295 0.10 -0.6 112,793 - BPHYT_RS20295 0.10 -0.4 112,793 - BPHYT_RS20295 0.10 -2.5 112,879 + BPHYT_RS20295 0.14 -0.2 113,051 - BPHYT_RS20295 0.22 -0.7 113,051 - BPHYT_RS20295 0.22 -0.3 113,056 + BPHYT_RS20295 0.22 +0.1 113,164 + BPHYT_RS20295 0.27 +0.4 113,215 + BPHYT_RS20295 0.30 +0.6 113,216 - BPHYT_RS20295 0.30 +1.1 113,216 - BPHYT_RS20295 0.30 -0.5 113,216 - BPHYT_RS20295 0.30 -0.8 113,225 - BPHYT_RS20295 0.30 +0.4 113,291 - BPHYT_RS20295 0.33 +0.2 113,321 - BPHYT_RS20295 0.35 +0.3 113,343 - BPHYT_RS20295 0.36 -0.5 113,379 - BPHYT_RS20295 0.37 -0.2 113,379 - BPHYT_RS20295 0.37 -0.6 113,379 - BPHYT_RS20295 0.37 +0.2 113,379 - BPHYT_RS20295 0.37 +0.1 113,509 + BPHYT_RS20295 0.43 +0.5 113,510 - BPHYT_RS20295 0.43 -0.1 113,539 + BPHYT_RS20295 0.45 -0.4
Or see this region's nucleotide sequence