Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS18805

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS18800 and BPHYT_RS18805 are separated by 485 nucleotidesBPHYT_RS18805 and BPHYT_RS18810 are separated by 65 nucleotides BPHYT_RS18800: BPHYT_RS18800 - flagellar hook-length control protein, at 4,226,728 to 4,228,221 _RS18800 BPHYT_RS18805: BPHYT_RS18805 - flagellar basal body protein FliL, at 4,228,707 to 4,229,207 _RS18805 BPHYT_RS18810: BPHYT_RS18810 - flagellar motor switch protein FliM, at 4,229,273 to 4,230,271 _RS18810 Position (kb) 4228 4229 4230Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 4227.774 kb on + strand, within BPHYT_RS18800at 4227.775 kb on - strand, within BPHYT_RS18800at 4227.775 kb on - strand, within BPHYT_RS18800at 4227.899 kb on + strand, within BPHYT_RS18800at 4227.900 kb on - strand, within BPHYT_RS18800at 4228.178 kb on + strandat 4228.179 kb on - strandat 4228.294 kb on + strandat 4228.499 kb on - strandat 4228.499 kb on - strandat 4228.524 kb on + strandat 4228.555 kb on + strandat 4228.555 kb on + strandat 4228.612 kb on + strandat 4228.684 kb on + strandat 4228.793 kb on + strand, within BPHYT_RS18805at 4228.979 kb on - strand, within BPHYT_RS18805at 4228.979 kb on - strand, within BPHYT_RS18805at 4229.032 kb on + strand, within BPHYT_RS18805at 4229.032 kb on + strand, within BPHYT_RS18805at 4229.033 kb on - strand, within BPHYT_RS18805at 4229.267 kb on + strandat 4229.268 kb on - strandat 4229.268 kb on - strandat 4229.268 kb on - strandat 4229.268 kb on - strandat 4229.385 kb on - strand, within BPHYT_RS18810at 4229.537 kb on + strand, within BPHYT_RS18810at 4229.617 kb on - strand, within BPHYT_RS18810at 4229.765 kb on + strand, within BPHYT_RS18810at 4229.765 kb on + strand, within BPHYT_RS18810at 4229.765 kb on + strand, within BPHYT_RS18810at 4229.765 kb on + strand, within BPHYT_RS18810at 4229.765 kb on + strand, within BPHYT_RS18810at 4229.766 kb on - strand, within BPHYT_RS18810at 4229.766 kb on - strand, within BPHYT_RS18810at 4229.917 kb on + strand, within BPHYT_RS18810at 4229.917 kb on + strand, within BPHYT_RS18810at 4229.918 kb on - strand, within BPHYT_RS18810at 4229.918 kb on - strand, within BPHYT_RS18810at 4229.918 kb on - strand, within BPHYT_RS18810at 4229.918 kb on - strand, within BPHYT_RS18810at 4229.918 kb on - strand, within BPHYT_RS18810at 4230.207 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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4,227,774 + BPHYT_RS18800 0.70 -2.2
4,227,775 - BPHYT_RS18800 0.70 -4.3
4,227,775 - BPHYT_RS18800 0.70 -2.4
4,227,899 + BPHYT_RS18800 0.78 -4.1
4,227,900 - BPHYT_RS18800 0.78 -2.5
4,228,178 + -2.6
4,228,179 - -0.6
4,228,294 + +1.1
4,228,499 - -0.7
4,228,499 - -0.0
4,228,524 + -0.0
4,228,555 + +0.6
4,228,555 + -0.2
4,228,612 + +0.2
4,228,684 + +0.1
4,228,793 + BPHYT_RS18805 0.17 -1.8
4,228,979 - BPHYT_RS18805 0.54 -1.8
4,228,979 - BPHYT_RS18805 0.54 -3.2
4,229,032 + BPHYT_RS18805 0.65 -0.2
4,229,032 + BPHYT_RS18805 0.65 -0.2
4,229,033 - BPHYT_RS18805 0.65 -3.2
4,229,267 + -4.6
4,229,268 - -2.3
4,229,268 - -1.7
4,229,268 - -4.3
4,229,268 - -1.4
4,229,385 - BPHYT_RS18810 0.11 -3.8
4,229,537 + BPHYT_RS18810 0.26 -4.1
4,229,617 - BPHYT_RS18810 0.34 -4.4
4,229,765 + BPHYT_RS18810 0.49 -4.4
4,229,765 + BPHYT_RS18810 0.49 -4.7
4,229,765 + BPHYT_RS18810 0.49 -0.4
4,229,765 + BPHYT_RS18810 0.49 -5.0
4,229,765 + BPHYT_RS18810 0.49 -1.3
4,229,766 - BPHYT_RS18810 0.49 +2.5
4,229,766 - BPHYT_RS18810 0.49 -2.5
4,229,917 + BPHYT_RS18810 0.64 -2.5
4,229,917 + BPHYT_RS18810 0.64 -1.4
4,229,918 - BPHYT_RS18810 0.65 -1.0
4,229,918 - BPHYT_RS18810 0.65 -1.7
4,229,918 - BPHYT_RS18810 0.65 -2.2
4,229,918 - BPHYT_RS18810 0.65 -3.2
4,229,918 - BPHYT_RS18810 0.65 -0.9
4,230,207 - -2.8

Or see this region's nucleotide sequence