Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17895 and BPHYT_RS17900 overlap by 4 nucleotides BPHYT_RS17900 and BPHYT_RS17905 overlap by 4 nucleotides BPHYT_RS17905 and BPHYT_RS17910 overlap by 4 nucleotides
BPHYT_RS17895: BPHYT_RS17895 - secretin, at 4,040,407 to 4,042,185
_RS17895
BPHYT_RS17900: BPHYT_RS17900 - hypothetical protein, at 4,042,182 to 4,043,114
_RS17900
BPHYT_RS17905: BPHYT_RS17905 - fimbrial assembly family protein, at 4,043,111 to 4,043,830
_RS17905
BPHYT_RS17910: BPHYT_RS17910 - hypothetical protein, at 4,043,827 to 4,044,819
_RS17910
Position (kb)
4042
4043
4044 Strain fitness (log2 ratio)
-2
-1
0
1 at 4041.284 kb on + strand, within BPHYT_RS17895 at 4041.284 kb on + strand, within BPHYT_RS17895 at 4041.987 kb on - strand, within BPHYT_RS17895 at 4042.028 kb on + strand at 4042.029 kb on - strand at 4042.114 kb on + strand at 4042.137 kb on + strand at 4042.138 kb on - strand at 4042.158 kb on + strand at 4042.159 kb on - strand at 4042.161 kb on - strand at 4042.587 kb on + strand, within BPHYT_RS17900 at 4042.677 kb on - strand, within BPHYT_RS17900 at 4042.677 kb on - strand, within BPHYT_RS17900 at 4043.108 kb on + strand at 4043.414 kb on + strand, within BPHYT_RS17905 at 4043.459 kb on + strand, within BPHYT_RS17905 at 4043.459 kb on + strand, within BPHYT_RS17905 at 4043.466 kb on - strand, within BPHYT_RS17905 at 4043.959 kb on + strand, within BPHYT_RS17910 at 4043.960 kb on - strand, within BPHYT_RS17910
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 4,041,284 + BPHYT_RS17895 0.49 -0.8 4,041,284 + BPHYT_RS17895 0.49 -1.1 4,041,987 - BPHYT_RS17895 0.89 +0.3 4,042,028 + -0.1 4,042,029 - +0.1 4,042,114 + -1.3 4,042,137 + +0.4 4,042,138 - +0.1 4,042,158 + -0.1 4,042,159 - -0.0 4,042,161 - +0.4 4,042,587 + BPHYT_RS17900 0.43 -1.1 4,042,677 - BPHYT_RS17900 0.53 -1.7 4,042,677 - BPHYT_RS17900 0.53 -1.7 4,043,108 + +1.1 4,043,414 + BPHYT_RS17905 0.42 -1.1 4,043,459 + BPHYT_RS17905 0.48 -0.3 4,043,459 + BPHYT_RS17905 0.48 -0.2 4,043,466 - BPHYT_RS17905 0.49 -1.8 4,043,959 + BPHYT_RS17910 0.13 -0.1 4,043,960 - BPHYT_RS17910 0.13 -1.7
Or see this region's nucleotide sequence