Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS16715

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS16710 and BPHYT_RS16715 are separated by 442 nucleotidesBPHYT_RS16715 and BPHYT_RS16720 are separated by 89 nucleotidesBPHYT_RS16720 and BPHYT_RS16725 are separated by 10 nucleotides BPHYT_RS16710: BPHYT_RS16710 - LysR family transcriptional regulator, at 3,782,671 to 3,783,627 _RS16710 BPHYT_RS16715: BPHYT_RS16715 - adenylosuccinate lyase, at 3,784,070 to 3,785,458 _RS16715 BPHYT_RS16720: BPHYT_RS16720 - Gluconokinase (EC 2.7.1.12) (from data), at 3,785,548 to 3,786,045 _RS16720 BPHYT_RS16725: BPHYT_RS16725 - gluconate:H+ symporter (gntT) (from data), at 3,786,056 to 3,787,453 _RS16725 Position (kb) 3784 3785 3786Strain fitness (log2 ratio) -2 -1 0 1 2at 3783.880 kb on - strandat 3783.921 kb on + strandat 3783.939 kb on + strandat 3785.456 kb on + strandat 3785.653 kb on - strand, within BPHYT_RS16720at 3785.745 kb on - strand, within BPHYT_RS16720at 3785.745 kb on - strand, within BPHYT_RS16720at 3786.058 kb on - strandat 3786.058 kb on - strandat 3786.162 kb on + strandat 3786.162 kb on + strandat 3786.163 kb on - strandat 3786.163 kb on - strandat 3786.163 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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3,783,880 - +1.9
3,783,921 + +0.2
3,783,939 + -0.3
3,785,456 + -2.5
3,785,653 - BPHYT_RS16720 0.21 +0.4
3,785,745 - BPHYT_RS16720 0.40 -0.9
3,785,745 - BPHYT_RS16720 0.40 -0.3
3,786,058 - -0.3
3,786,058 - +1.4
3,786,162 + -0.6
3,786,162 + -0.7
3,786,163 - +0.4
3,786,163 - +0.4
3,786,163 - +1.2

Or see this region's nucleotide sequence