Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS15135

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS15130 and BPHYT_RS15135 overlap by 1 nucleotidesBPHYT_RS15135 and BPHYT_RS15140 overlap by 4 nucleotidesBPHYT_RS15140 and BPHYT_RS15145 are separated by 32 nucleotides BPHYT_RS15130: BPHYT_RS15130 - 3-oxoacyl-ACP synthase, at 3,443,047 to 3,444,273 _RS15130 BPHYT_RS15135: BPHYT_RS15135 - 3-ketoacyl-ACP reductase, at 3,444,273 to 3,444,998 _RS15135 BPHYT_RS15140: BPHYT_RS15140 - beta-hydroxyacyl-ACP dehydratase, at 3,444,995 to 3,445,480 _RS15140 BPHYT_RS15145: BPHYT_RS15145 - 3-oxoacyl-ACP synthase, at 3,445,513 to 3,446,694 _RS15145 Position (kb) 3444 3445Strain fitness (log2 ratio) -2 -1 0 1at 3443.279 kb on + strand, within BPHYT_RS15130at 3443.279 kb on + strand, within BPHYT_RS15130at 3443.546 kb on + strand, within BPHYT_RS15130at 3443.547 kb on - strand, within BPHYT_RS15130at 3443.547 kb on - strand, within BPHYT_RS15130at 3443.657 kb on + strandat 3443.751 kb on - strand, within BPHYT_RS15130at 3443.870 kb on + strand, within BPHYT_RS15130at 3443.871 kb on - strand, within BPHYT_RS15130at 3443.871 kb on - strand, within BPHYT_RS15130at 3443.961 kb on - strand, within BPHYT_RS15130at 3444.047 kb on + strand, within BPHYT_RS15130at 3444.047 kb on + strand, within BPHYT_RS15130at 3444.048 kb on - strand, within BPHYT_RS15130at 3444.153 kb on - strandat 3444.153 kb on - strandat 3444.316 kb on + strandat 3444.316 kb on + strandat 3444.317 kb on - strandat 3444.541 kb on + strand, within BPHYT_RS15135at 3444.744 kb on + strand, within BPHYT_RS15135at 3444.745 kb on - strand, within BPHYT_RS15135at 3444.745 kb on - strand, within BPHYT_RS15135at 3444.754 kb on + strand, within BPHYT_RS15135at 3444.940 kb on + strandat 3445.192 kb on - strand, within BPHYT_RS15140at 3445.329 kb on + strand, within BPHYT_RS15140at 3445.491 kb on - strandat 3445.575 kb on - strandat 3445.856 kb on + strand, within BPHYT_RS15145

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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3,443,279 + BPHYT_RS15130 0.19 -1.2
3,443,279 + BPHYT_RS15130 0.19 +0.6
3,443,546 + BPHYT_RS15130 0.41 -0.5
3,443,547 - BPHYT_RS15130 0.41 +0.4
3,443,547 - BPHYT_RS15130 0.41 +1.2
3,443,657 + +1.4
3,443,751 - BPHYT_RS15130 0.57 -0.2
3,443,870 + BPHYT_RS15130 0.67 +0.1
3,443,871 - BPHYT_RS15130 0.67 +0.2
3,443,871 - BPHYT_RS15130 0.67 +0.2
3,443,961 - BPHYT_RS15130 0.74 -2.2
3,444,047 + BPHYT_RS15130 0.81 +0.0
3,444,047 + BPHYT_RS15130 0.81 +0.7
3,444,048 - BPHYT_RS15130 0.82 -0.6
3,444,153 - +1.0
3,444,153 - +0.2
3,444,316 + +0.2
3,444,316 + +0.5
3,444,317 - -0.2
3,444,541 + BPHYT_RS15135 0.37 -0.5
3,444,744 + BPHYT_RS15135 0.65 +0.0
3,444,745 - BPHYT_RS15135 0.65 +1.0
3,444,745 - BPHYT_RS15135 0.65 +0.7
3,444,754 + BPHYT_RS15135 0.66 +0.7
3,444,940 + +0.0
3,445,192 - BPHYT_RS15140 0.41 +0.2
3,445,329 + BPHYT_RS15140 0.69 -0.2
3,445,491 - -1.0
3,445,575 - -0.7
3,445,856 + BPHYT_RS15145 0.29 -0.6

Or see this region's nucleotide sequence