Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS14865

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS14860 and BPHYT_RS14865 are separated by 49 nucleotidesBPHYT_RS14865 and BPHYT_RS14870 are separated by 150 nucleotides BPHYT_RS14860: BPHYT_RS14860 - sugar kinase, at 3,379,889 to 3,380,809 _RS14860 BPHYT_RS14865: BPHYT_RS14865 - UDP-glucose 6-dehydrogenase, at 3,380,859 to 3,382,262 _RS14865 BPHYT_RS14870: BPHYT_RS14870 - hypothetical protein, at 3,382,413 to 3,383,588 _RS14870 Position (kb) 3380 3381 3382 3383Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3380.893 kb on + strandat 3381.608 kb on - strand, within BPHYT_RS14865at 3381.641 kb on - strand, within BPHYT_RS14865at 3381.971 kb on - strand, within BPHYT_RS14865at 3382.235 kb on - strandat 3382.454 kb on - strandat 3382.454 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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3,380,893 + -5.6
3,381,608 - BPHYT_RS14865 0.53 -2.8
3,381,641 - BPHYT_RS14865 0.56 -4.0
3,381,971 - BPHYT_RS14865 0.79 -4.5
3,382,235 - -4.7
3,382,454 - -0.1
3,382,454 - +1.0

Or see this region's nucleotide sequence