Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS14620

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS14615 and BPHYT_RS14620 are separated by 129 nucleotidesBPHYT_RS14620 and BPHYT_RS14625 are separated by 145 nucleotides BPHYT_RS14615: BPHYT_RS14615 - fumarate hydratase, at 3,329,189 to 3,330,589 _RS14615 BPHYT_RS14620: BPHYT_RS14620 - acyl-CoA hydrolase, at 3,330,719 to 3,331,225 _RS14620 BPHYT_RS14625: BPHYT_RS14625 - transcriptional regulator, at 3,331,371 to 3,332,684 _RS14625 Position (kb) 3330 3331 3332Strain fitness (log2 ratio) -1 0 1at 3329.858 kb on + strand, within BPHYT_RS14615at 3329.859 kb on - strand, within BPHYT_RS14615at 3329.859 kb on - strand, within BPHYT_RS14615at 3330.016 kb on - strand, within BPHYT_RS14615at 3330.118 kb on - strand, within BPHYT_RS14615at 3330.565 kb on + strandat 3330.566 kb on - strandat 3330.587 kb on + strandat 3330.789 kb on - strand, within BPHYT_RS14620at 3331.062 kb on + strandat 3331.092 kb on + strand, within BPHYT_RS14620at 3331.093 kb on - strand, within BPHYT_RS14620at 3331.265 kb on + strandat 3331.268 kb on - strandat 3332.072 kb on - strand, within BPHYT_RS14625at 3332.072 kb on - strand, within BPHYT_RS14625

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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3,329,858 + BPHYT_RS14615 0.48 -0.3
3,329,859 - BPHYT_RS14615 0.48 +0.4
3,329,859 - BPHYT_RS14615 0.48 -1.1
3,330,016 - BPHYT_RS14615 0.59 -0.5
3,330,118 - BPHYT_RS14615 0.66 -1.1
3,330,565 + -0.2
3,330,566 - -0.1
3,330,587 + -0.0
3,330,789 - BPHYT_RS14620 0.14 +1.7
3,331,062 + +1.0
3,331,092 + BPHYT_RS14620 0.74 +0.1
3,331,093 - BPHYT_RS14620 0.74 +0.4
3,331,265 + +0.2
3,331,268 - -1.5
3,332,072 - BPHYT_RS14625 0.53 +0.3
3,332,072 - BPHYT_RS14625 0.53 -0.2

Or see this region's nucleotide sequence