Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS13310

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS13305 and BPHYT_RS13310 are separated by 144 nucleotidesBPHYT_RS13310 and BPHYT_RS13315 are separated by 68 nucleotides BPHYT_RS13305: BPHYT_RS13305 - nitrate reductase, at 3,037,156 to 3,038,667 _RS13305 BPHYT_RS13310: BPHYT_RS13310 - dihydropyrimidine dehydrogenase subunit B, at 3,038,812 to 3,040,146 _RS13310 BPHYT_RS13315: BPHYT_RS13315 - dihydropyrimidine dehydrogenase subunit A, at 3,040,215 to 3,041,564 _RS13315 Position (kb) 3038 3039 3040 3041Strain fitness (log2 ratio) -1 0 1at 3038.128 kb on + strand, within BPHYT_RS13305at 3038.249 kb on + strand, within BPHYT_RS13305at 3038.249 kb on + strand, within BPHYT_RS13305at 3038.505 kb on - strand, within BPHYT_RS13305at 3038.604 kb on - strandat 3038.604 kb on - strandat 3039.091 kb on - strand, within BPHYT_RS13310at 3039.575 kb on + strand, within BPHYT_RS13310at 3040.061 kb on + strandat 3040.129 kb on - strandat 3040.182 kb on + strandat 3040.228 kb on - strandat 3040.228 kb on - strandat 3040.776 kb on + strand, within BPHYT_RS13315

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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3,038,128 + BPHYT_RS13305 0.64 +0.5
3,038,249 + BPHYT_RS13305 0.72 -0.1
3,038,249 + BPHYT_RS13305 0.72 -0.4
3,038,505 - BPHYT_RS13305 0.89 +0.6
3,038,604 - -0.9
3,038,604 - +0.3
3,039,091 - BPHYT_RS13310 0.21 +1.2
3,039,575 + BPHYT_RS13310 0.57 +0.2
3,040,061 + +1.1
3,040,129 - +0.1
3,040,182 + -0.3
3,040,228 - +0.6
3,040,228 - -0.1
3,040,776 + BPHYT_RS13315 0.42 +0.3

Or see this region's nucleotide sequence