Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13190 and BPHYT_RS13195 are separated by 364 nucleotides BPHYT_RS13195 and BPHYT_RS13200 are separated by 117 nucleotides BPHYT_RS13200 and BPHYT_RS13205 are separated by 40 nucleotides BPHYT_RS13205 and BPHYT_RS13210 are separated by 30 nucleotides
BPHYT_RS13190: BPHYT_RS13190 - membrane protein, at 3,010,573 to 3,011,502
_RS13190
BPHYT_RS13195: BPHYT_RS13195 - hypothetical protein, at 3,011,867 to 3,012,112
_RS13195
BPHYT_RS13200: BPHYT_RS13200 - molybdopterin binding oxidoreductase, at 3,012,230 to 3,013,021
_RS13200
BPHYT_RS13205: BPHYT_RS13205 - cytochrome B561, at 3,013,062 to 3,013,703
_RS13205
BPHYT_RS13210: BPHYT_RS13210 - hypothetical protein, at 3,013,734 to 3,014,579
_RS13210
Position (kb) 3012
3013
3014 Strain fitness (log2 ratio) -2
-1
0
1 at 3011.308 kb on + strand, within BPHYT_RS13190 at 3011.384 kb on + strand, within BPHYT_RS13190 at 3011.731 kb on + strand at 3011.732 kb on - strand at 3012.153 kb on - strand at 3012.454 kb on - strand, within BPHYT_RS13200 at 3012.483 kb on + strand, within BPHYT_RS13200 at 3013.304 kb on + strand, within BPHYT_RS13205
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,011,308 + BPHYT_RS13190 0.79 -0.4 3,011,384 + BPHYT_RS13190 0.87 +0.4 3,011,731 + -0.1 3,011,732 - -0.0 3,012,153 - +0.4 3,012,454 - BPHYT_RS13200 0.28 -1.4 3,012,483 + BPHYT_RS13200 0.32 -0.4 3,013,304 + BPHYT_RS13205 0.38 -2.0
Or see this region's nucleotide sequence