Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13050 and BPHYT_RS13055 are separated by 267 nucleotides BPHYT_RS13055 and BPHYT_RS13060 are separated by 29 nucleotides BPHYT_RS13060 and BPHYT_RS13065 are separated by 20 nucleotides
BPHYT_RS13050: BPHYT_RS13050 - membrane protein, at 2,983,221 to 2,984,351
_RS13050
BPHYT_RS13055: BPHYT_RS13055 - short-chain dehydrogenase, at 2,984,619 to 2,985,368
_RS13055
BPHYT_RS13060: BPHYT_RS13060 - hypothetical protein, at 2,985,398 to 2,985,724
_RS13060
BPHYT_RS13065: BPHYT_RS13065 - 2-aminoadipate aminotransferase, at 2,985,745 to 2,987,178
_RS13065
Position (kb)
2984
2985
2986 Strain fitness (log2 ratio)
-2
-1
0
1 at 2983.730 kb on + strand, within BPHYT_RS13050 at 2983.731 kb on - strand, within BPHYT_RS13050 at 2983.888 kb on + strand, within BPHYT_RS13050 at 2983.915 kb on + strand, within BPHYT_RS13050 at 2983.916 kb on - strand, within BPHYT_RS13050 at 2983.916 kb on - strand, within BPHYT_RS13050 at 2983.916 kb on - strand, within BPHYT_RS13050 at 2983.916 kb on - strand, within BPHYT_RS13050 at 2984.637 kb on - strand at 2984.965 kb on - strand, within BPHYT_RS13055 at 2985.724 kb on + strand at 2985.724 kb on + strand at 2985.819 kb on - strand at 2985.819 kb on - strand at 2985.819 kb on - strand at 2985.819 kb on - strand at 2985.819 kb on - strand at 2985.819 kb on - strand at 2985.957 kb on - strand, within BPHYT_RS13065 at 2985.957 kb on - strand, within BPHYT_RS13065 at 2986.040 kb on + strand, within BPHYT_RS13065 at 2986.074 kb on - strand, within BPHYT_RS13065 at 2986.092 kb on - strand, within BPHYT_RS13065 at 2986.134 kb on + strand, within BPHYT_RS13065 at 2986.134 kb on + strand, within BPHYT_RS13065 at 2986.170 kb on - strand, within BPHYT_RS13065 at 2986.295 kb on + strand, within BPHYT_RS13065 at 2986.295 kb on + strand, within BPHYT_RS13065 at 2986.296 kb on - strand, within BPHYT_RS13065 at 2986.296 kb on - strand, within BPHYT_RS13065 at 2986.331 kb on - strand, within BPHYT_RS13065
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,983,730 + BPHYT_RS13050 0.45 -0.3 2,983,731 - BPHYT_RS13050 0.45 +0.2 2,983,888 + BPHYT_RS13050 0.59 -0.3 2,983,915 + BPHYT_RS13050 0.61 -1.8 2,983,916 - BPHYT_RS13050 0.61 -0.2 2,983,916 - BPHYT_RS13050 0.61 +0.5 2,983,916 - BPHYT_RS13050 0.61 +0.5 2,983,916 - BPHYT_RS13050 0.61 -0.2 2,984,637 - -0.2 2,984,965 - BPHYT_RS13055 0.46 +0.2 2,985,724 + +0.5 2,985,724 + -0.5 2,985,819 - +0.2 2,985,819 - +0.3 2,985,819 - +0.2 2,985,819 - -1.2 2,985,819 - -0.0 2,985,819 - +0.2 2,985,957 - BPHYT_RS13065 0.15 +0.5 2,985,957 - BPHYT_RS13065 0.15 +0.1 2,986,040 + BPHYT_RS13065 0.21 -1.7 2,986,074 - BPHYT_RS13065 0.23 -0.2 2,986,092 - BPHYT_RS13065 0.24 -0.1 2,986,134 + BPHYT_RS13065 0.27 +1.3 2,986,134 + BPHYT_RS13065 0.27 -0.9 2,986,170 - BPHYT_RS13065 0.30 +0.4 2,986,295 + BPHYT_RS13065 0.38 +0.0 2,986,295 + BPHYT_RS13065 0.38 -0.1 2,986,296 - BPHYT_RS13065 0.38 -0.0 2,986,296 - BPHYT_RS13065 0.38 -0.1 2,986,331 - BPHYT_RS13065 0.41 -1.5
Or see this region's nucleotide sequence