Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS12480

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS12475 and BPHYT_RS12480 overlap by 14 nucleotidesBPHYT_RS12480 and BPHYT_RS12485 overlap by 21 nucleotidesBPHYT_RS12485 and BPHYT_RS12490 are separated by 32 nucleotides BPHYT_RS12475: BPHYT_RS12475 - DNA mismatch repair protein MutS, at 2,843,553 to 2,846,237 _RS12475 BPHYT_RS12480: BPHYT_RS12480 - hypothetical protein, at 2,846,224 to 2,847,582 _RS12480 BPHYT_RS12485: BPHYT_RS12485 - peptidyl-prolyl cis-trans isomerase, at 2,847,562 to 2,848,104 _RS12485 BPHYT_RS12490: BPHYT_RS12490 - cupin, at 2,848,137 to 2,849,387 _RS12490 Position (kb) 2846 2847 2848Strain fitness (log2 ratio) -3 -2 -1 0 1at 2845.509 kb on + strand, within BPHYT_RS12475at 2845.510 kb on - strand, within BPHYT_RS12475at 2845.521 kb on + strand, within BPHYT_RS12475at 2845.521 kb on + strand, within BPHYT_RS12475at 2845.522 kb on - strand, within BPHYT_RS12475at 2845.773 kb on - strand, within BPHYT_RS12475at 2845.777 kb on + strand, within BPHYT_RS12475at 2845.777 kb on + strand, within BPHYT_RS12475at 2846.365 kb on + strand, within BPHYT_RS12480at 2846.383 kb on + strandat 2846.492 kb on - strand, within BPHYT_RS12480at 2846.695 kb on - strand, within BPHYT_RS12480at 2846.695 kb on - strand, within BPHYT_RS12480at 2846.834 kb on - strand, within BPHYT_RS12480at 2847.222 kb on + strand, within BPHYT_RS12480at 2847.896 kb on + strand, within BPHYT_RS12485at 2847.897 kb on - strand, within BPHYT_RS12485at 2847.995 kb on + strand, within BPHYT_RS12485at 2847.996 kb on - strand, within BPHYT_RS12485at 2848.010 kb on + strand, within BPHYT_RS12485at 2848.010 kb on + strand, within BPHYT_RS12485at 2848.067 kb on + strandat 2848.068 kb on - strandat 2848.120 kb on - strandat 2848.132 kb on - strandat 2848.133 kb on + strandat 2848.133 kb on + strandat 2848.134 kb on - strandat 2848.134 kb on - strandat 2848.134 kb on - strandat 2848.134 kb on - strandat 2848.421 kb on + strand, within BPHYT_RS12490at 2848.422 kb on - strand, within BPHYT_RS12490

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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2,845,509 + BPHYT_RS12475 0.73 +0.8
2,845,510 - BPHYT_RS12475 0.73 +0.0
2,845,521 + BPHYT_RS12475 0.73 +0.9
2,845,521 + BPHYT_RS12475 0.73 +0.1
2,845,522 - BPHYT_RS12475 0.73 +0.5
2,845,773 - BPHYT_RS12475 0.83 -1.2
2,845,777 + BPHYT_RS12475 0.83 +0.1
2,845,777 + BPHYT_RS12475 0.83 -0.9
2,846,365 + BPHYT_RS12480 0.10 -0.7
2,846,383 + +0.9
2,846,492 - BPHYT_RS12480 0.20 -0.6
2,846,695 - BPHYT_RS12480 0.35 -1.3
2,846,695 - BPHYT_RS12480 0.35 +0.2
2,846,834 - BPHYT_RS12480 0.45 -0.5
2,847,222 + BPHYT_RS12480 0.73 +0.4
2,847,896 + BPHYT_RS12485 0.62 +0.1
2,847,897 - BPHYT_RS12485 0.62 -0.4
2,847,995 + BPHYT_RS12485 0.80 +0.7
2,847,996 - BPHYT_RS12485 0.80 +1.1
2,848,010 + BPHYT_RS12485 0.83 -0.1
2,848,010 + BPHYT_RS12485 0.83 -0.1
2,848,067 + +0.0
2,848,068 - -2.8
2,848,120 - -0.8
2,848,132 - +0.0
2,848,133 + +0.1
2,848,133 + -0.8
2,848,134 - -1.0
2,848,134 - -1.0
2,848,134 - +0.4
2,848,134 - -2.6
2,848,421 + BPHYT_RS12490 0.23 +0.6
2,848,422 - BPHYT_RS12490 0.23 -0.3

Or see this region's nucleotide sequence