Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS11525

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS11520 and BPHYT_RS11525 are separated by 13 nucleotidesBPHYT_RS11525 and BPHYT_RS11530 are separated by 340 nucleotides BPHYT_RS11520: BPHYT_RS11520 - hydroxydechloroatrazine ethylaminohydrolase, at 2,620,043 to 2,621,440 _RS11520 BPHYT_RS11525: BPHYT_RS11525 - hypothetical protein, at 2,621,454 to 2,621,636 _RS11525 BPHYT_RS11530: BPHYT_RS11530 - 5-hydroxyisourate hydrolase, at 2,621,977 to 2,622,330 _RS11530 Position (kb) 2621 2622Strain fitness (log2 ratio) -1 0 1 2at 2620.607 kb on + strand, within BPHYT_RS11520at 2620.608 kb on - strand, within BPHYT_RS11520at 2620.676 kb on - strand, within BPHYT_RS11520at 2621.014 kb on - strand, within BPHYT_RS11520at 2621.018 kb on - strand, within BPHYT_RS11520at 2621.019 kb on + strand, within BPHYT_RS11520at 2621.019 kb on + strand, within BPHYT_RS11520at 2621.019 kb on + strand, within BPHYT_RS11520at 2621.019 kb on + strand, within BPHYT_RS11520at 2621.019 kb on + strand, within BPHYT_RS11520at 2621.020 kb on - strand, within BPHYT_RS11520at 2621.020 kb on - strand, within BPHYT_RS11520at 2621.020 kb on - strand, within BPHYT_RS11520at 2621.020 kb on - strand, within BPHYT_RS11520at 2621.193 kb on + strand, within BPHYT_RS11520at 2621.193 kb on + strand, within BPHYT_RS11520at 2621.319 kb on + strandat 2621.320 kb on - strandat 2621.621 kb on - strandat 2621.639 kb on + strandat 2621.689 kb on + strandat 2621.689 kb on + strandat 2621.689 kb on + strandat 2621.741 kb on - strandat 2621.799 kb on + strandat 2621.799 kb on + strandat 2621.800 kb on - strandat 2621.844 kb on - strandat 2621.885 kb on - strandat 2621.925 kb on - strandat 2622.274 kb on + strand, within BPHYT_RS11530at 2622.282 kb on - strand, within BPHYT_RS11530at 2622.307 kb on + strandat 2622.308 kb on - strandat 2622.627 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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2,620,607 + BPHYT_RS11520 0.40 +0.7
2,620,608 - BPHYT_RS11520 0.40 -0.3
2,620,676 - BPHYT_RS11520 0.45 +1.9
2,621,014 - BPHYT_RS11520 0.69 -0.5
2,621,018 - BPHYT_RS11520 0.70 -0.1
2,621,019 + BPHYT_RS11520 0.70 +0.2
2,621,019 + BPHYT_RS11520 0.70 +1.4
2,621,019 + BPHYT_RS11520 0.70 -0.7
2,621,019 + BPHYT_RS11520 0.70 +0.2
2,621,019 + BPHYT_RS11520 0.70 +1.0
2,621,020 - BPHYT_RS11520 0.70 -0.7
2,621,020 - BPHYT_RS11520 0.70 +2.5
2,621,020 - BPHYT_RS11520 0.70 -0.5
2,621,020 - BPHYT_RS11520 0.70 +0.5
2,621,193 + BPHYT_RS11520 0.82 -0.8
2,621,193 + BPHYT_RS11520 0.82 +0.0
2,621,319 + -0.1
2,621,320 - -0.1
2,621,621 - +0.7
2,621,639 + +0.1
2,621,689 + +0.2
2,621,689 + -1.1
2,621,689 + -0.5
2,621,741 - -0.1
2,621,799 + -0.3
2,621,799 + +1.2
2,621,800 - -1.1
2,621,844 - +0.5
2,621,885 - -0.7
2,621,925 - -0.1
2,622,274 + BPHYT_RS11530 0.84 +0.2
2,622,282 - BPHYT_RS11530 0.86 -1.2
2,622,307 + -0.2
2,622,308 - +0.4
2,622,627 - -0.6

Or see this region's nucleotide sequence