Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS11275

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS11265 and BPHYT_RS11270 are separated by 298 nucleotidesBPHYT_RS11270 and BPHYT_RS11275 are separated by 45 nucleotidesBPHYT_RS11275 and BPHYT_RS11280 are separated by 154 nucleotides BPHYT_RS11265: BPHYT_RS11265 - hypothetical protein, at 2,560,012 to 2,560,527 _RS11265 BPHYT_RS11270: BPHYT_RS11270 - hypothetical protein, at 2,560,826 to 2,561,263 _RS11270 BPHYT_RS11275: BPHYT_RS11275 - hypothetical protein, at 2,561,309 to 2,561,677 _RS11275 BPHYT_RS11280: BPHYT_RS11280 - membrane protein, at 2,561,832 to 2,562,650 _RS11280 Position (kb) 2561 2562Strain fitness (log2 ratio) -1 0 1at 2560.316 kb on - strand, within BPHYT_RS11265at 2560.526 kb on - strandat 2560.526 kb on - strandat 2560.530 kb on - strandat 2560.581 kb on + strandat 2561.033 kb on + strand, within BPHYT_RS11270at 2561.033 kb on + strand, within BPHYT_RS11270at 2561.034 kb on - strand, within BPHYT_RS11270at 2561.246 kb on + strandat 2561.247 kb on - strandat 2561.370 kb on + strand, within BPHYT_RS11275at 2561.370 kb on + strand, within BPHYT_RS11275at 2561.371 kb on - strand, within BPHYT_RS11275at 2561.515 kb on + strand, within BPHYT_RS11275at 2561.665 kb on - strandat 2561.858 kb on - strandat 2561.902 kb on + strandat 2561.902 kb on + strandat 2561.903 kb on - strandat 2561.903 kb on - strandat 2561.903 kb on - strandat 2561.903 kb on - strandat 2561.998 kb on - strand, within BPHYT_RS11280at 2562.406 kb on + strand, within BPHYT_RS11280at 2562.406 kb on + strand, within BPHYT_RS11280at 2562.469 kb on + strand, within BPHYT_RS11280at 2562.470 kb on - strand, within BPHYT_RS11280at 2562.588 kb on + strandat 2562.632 kb on + strandat 2562.646 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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2,560,316 - BPHYT_RS11265 0.59 +0.9
2,560,526 - -0.0
2,560,526 - +0.3
2,560,530 - +0.8
2,560,581 + +0.6
2,561,033 + BPHYT_RS11270 0.47 -0.1
2,561,033 + BPHYT_RS11270 0.47 -0.3
2,561,034 - BPHYT_RS11270 0.47 -0.2
2,561,246 + +0.1
2,561,247 - -0.2
2,561,370 + BPHYT_RS11275 0.17 +1.5
2,561,370 + BPHYT_RS11275 0.17 +1.4
2,561,371 - BPHYT_RS11275 0.17 +0.3
2,561,515 + BPHYT_RS11275 0.56 -0.2
2,561,665 - -1.4
2,561,858 - -0.1
2,561,902 + -1.2
2,561,902 + -0.3
2,561,903 - +1.1
2,561,903 - +1.5
2,561,903 - -1.1
2,561,903 - +0.2
2,561,998 - BPHYT_RS11280 0.20 +0.1
2,562,406 + BPHYT_RS11280 0.70 -0.4
2,562,406 + BPHYT_RS11280 0.70 -0.3
2,562,469 + BPHYT_RS11280 0.78 +0.2
2,562,470 - BPHYT_RS11280 0.78 -0.5
2,562,588 + -1.4
2,562,632 + +0.6
2,562,646 - -0.5

Or see this region's nucleotide sequence